erum Hanif | University of Karachi (original) (raw)

Papers by erum Hanif

FUUAST Journal of Biology, Dec 13, 2014

FUUAST Journal of Biology, 2016

Pakistan Journal of Pharmaceutical Sciences, 2019

Staphylococcus aureus is a common skin colonizer as well as opportunistic pathogen causing seriou... more Staphylococcus aureus is a common skin colonizer as well as opportunistic pathogen causing serious diseases including bacteremia, endocarditis and a number of different infections. It has a unique ability to swiftly respond and develop resistance for every other antibiotic introduced against it. The prevalence of antibiotic resistant strains of S. aureus is increasing on an alarming rate, which not only restrains the treatment options but the economic deprivation sustained due to infections of this superbug are incomputable. In our study, antimicrobial resistance patterns for 13 different antibiotics were evaluated in non-duplicate isolates of MSSA and MRSA isolated from different clinical samples (i.e. urine, pus, HVS, blood, tissue, wound and ear swabs). Most cultures were identified as multi-drug resistant (MDR). The highest resistance was recorded against ampicillin and erythromycin (88% each), while resistances against oxacillin, fosfomycin, cefoxitin and ciprofloxacin were als...

Pakistan journal of pharmaceutical sciences, 2019

In recent years, silver nanoparticles have gained attention because of their high surface area to... more In recent years, silver nanoparticles have gained attention because of their high surface area to volume ratio that makes them more advantageous than their bulky counterparts. Apart of chemical and physical methods of silver nanoparticles (AgNPs) production, green synthesis is being exploited by the researchers. Aspergillus niger are among some fungi being used in fabrication of silver nanoparticles and their antifungal activities are being studied. We have experimented using A. niger Gin for extracellular silver nanoparticle synthesis. Characterization of AgNPs was done by UV-Visible spectroscopy and SEM-EDS.

Pakistan Journal of Chemistry

Microorganisms have numerous biotechnological applications for bioremediation of metal-contaminat... more Microorganisms have numerous biotechnological applications for bioremediation of metal-contaminated soil and water systems. In view of this, the present study was aimed to evaluate the abilities of microorganisms for tolerance and biosorption of heavy metals. In this study we have isolated 30 different bacterial strains from soil. Morphological and biochemical characterization was carried out. It was shown that Pseudomonas sp. was most common isolate among heavy metal resistant organisms that are capable of resisting 5 heavy metals (Ni, Pb, Cr, Cu and Cd) of concentration range 50-300µg/ml. Among 30 isolates, 4 of them showed high degree of resistance against all metals at concentration at 300µg/ml. Bacterial strains isolated in this study can be used in bioremediation and bio-mining.

Pakistan Journal of Chemistry

The present work focused on the production of extracellular lipase from Pseudomonas aeruginosa HE... more The present work focused on the production of extracellular lipase from Pseudomonas aeruginosa HE05 emplying natural oils of plants as substrat. Nine isolates (HE01-HE09) were isolated from the desiel oil contaminated soil and screened for lipase activity on nutrient agar medium supplemented with 1% olive oil and tween 80. Pseudomonas aeruginosa HE05 was identified as the hyperproducer of lipase. Maximum lipase production (3123U/ml) was recorded when 1% olive oil and 1% tween 80 added in nutrient broth used as a substrate in submerged fermentation. The optimal production was achevied in 96 hrs at 37 o C at 100 rpm. Quantification of lipase units were performed by turbidimetric assay. Supplementation of nutrient broth with natural oils was found as inducer as well as substrate for the production of lipase. Among the natural oils, mustard oil, corn oil, almond oil are better substrate for extracellular lipase production.

Glutamate decarboxylase (GAD) was isolated from a cell-free extract of E. coli JM109.and partiall... more Glutamate decarboxylase (GAD) was isolated from a cell-free extract of E. coli JM109.and partially purified by applying crude intracellular extract on Sephadex G-100. The enzyme was eluted in the first peak of the column and it was purified 27%. First peak showed highest GABA production activity on TLC plates. Absorption spectrum showed 0.33mg/ml of protein.

JPMA. The Journal of the Pakistan Medical Association, 2021

OBJECTIVE The main objective of the study was to evaluate the health of University of Karachi stu... more OBJECTIVE The main objective of the study was to evaluate the health of University of Karachi students (aged 19 to 21) by observing the correlation of haemoglobin level with underweight BMI. METHODS In this study 151 students were enrolled from the Department of Biotechnology, University of Karachi, Sindh Pakistan. Blood samples were collected and Haemoglobin estimation was performed by Sahli's Haemoglobinometer. BMI of each student was calculated as per WHO criteria for South Asian. Results Out of 151 students, 60 (39.7%) students had anaemia, among which 41(27.1%) were showing grade 1 anaemia and 19(12.5%) were showing grade 2 anaemia. BMI results showed that 50 (33.1%) students were underweight whereas, 19(12.5%) students were overweight and 12( 7.9%) students were obese. Correlation showed that 34 (22.5%) students had anaemia with underweight BMI. CONCLUSIONS This study concluded that anaemia with underweight BMI is more prevalent among females especially adolescent girls. T...

Pakistan journal of pharmaceutical sciences, 2019

Staphylococcus aureus is a common skin colonizer as well as opportunistic pathogen causing seriou... more Staphylococcus aureus is a common skin colonizer as well as opportunistic pathogen causing serious diseases including bacteremia, endocarditis and a number of different infections. It has a unique ability to swiftly respond and develop resistance for every other antibiotic introduced against it. The prevalence of antibiotic resistant strains of S. aureus is increasing on an alarming rate, which not only restrains the treatment options but the economic deprivation sustained due to infections of this superbug are incomputable. In our study, antimicrobial resistance patterns for 13 different antibiotics were evaluated in non-duplicate isolates of MSSA and MRSA isolated from different clinical samples (i.e. urine, pus, HVS, blood, tissue, wound and ear swabs). Most cultures were identified as multi-drug resistant (MDR). The highest resistance was recorded against ampicillin and erythromycin (88% each), while resistances against oxacillin, fosfomycin, cefoxitin and ciprofloxacin were als...

AUSTRALIAN JOURNAL OF BASIC AND APPLIED SCIENCES

Glucoamylase (EC 3.2.1.3) is exo-enzyme of extraordinary significance for saccharification of sta... more Glucoamylase (EC 3.2.1.3) is exo-enzyme of extraordinary significance for saccharification of starchy materials and other related oligosaccharides. Glucoamylase successively hydrolyzes 1,4-alpha glycosidic bond from the non-reducing end of starch and other related polysaccharides and release free glucose, which then used as a feedstock for the process of fermentation (Gupta et al., 2003; Norouzian et al., 2006). Industrial production of glucoamylase has been performed by the fungi, Aspergillus niger (Wang et al., 2006). Glucoamylase has different applications in significant areas of food processing, animals feed, fermentation biotechnology, paper making, fabric texture, grain hydrolysis for the alcohol industry (Zambare, 2011). The production of glucoamylase from different Aspergillus species including Aspergillus niger, Aspergillus fumigatus,

Journal of Basic & Applied Sciences

FUUAST Journal of Biology, Dec 13, 2014

FUUAST Journal of Biology, 2016

Pakistan Journal of Pharmaceutical Sciences, 2019

Staphylococcus aureus is a common skin colonizer as well as opportunistic pathogen causing seriou... more Staphylococcus aureus is a common skin colonizer as well as opportunistic pathogen causing serious diseases including bacteremia, endocarditis and a number of different infections. It has a unique ability to swiftly respond and develop resistance for every other antibiotic introduced against it. The prevalence of antibiotic resistant strains of S. aureus is increasing on an alarming rate, which not only restrains the treatment options but the economic deprivation sustained due to infections of this superbug are incomputable. In our study, antimicrobial resistance patterns for 13 different antibiotics were evaluated in non-duplicate isolates of MSSA and MRSA isolated from different clinical samples (i.e. urine, pus, HVS, blood, tissue, wound and ear swabs). Most cultures were identified as multi-drug resistant (MDR). The highest resistance was recorded against ampicillin and erythromycin (88% each), while resistances against oxacillin, fosfomycin, cefoxitin and ciprofloxacin were als...

Pakistan journal of pharmaceutical sciences, 2019

In recent years, silver nanoparticles have gained attention because of their high surface area to... more In recent years, silver nanoparticles have gained attention because of their high surface area to volume ratio that makes them more advantageous than their bulky counterparts. Apart of chemical and physical methods of silver nanoparticles (AgNPs) production, green synthesis is being exploited by the researchers. Aspergillus niger are among some fungi being used in fabrication of silver nanoparticles and their antifungal activities are being studied. We have experimented using A. niger Gin for extracellular silver nanoparticle synthesis. Characterization of AgNPs was done by UV-Visible spectroscopy and SEM-EDS.

Pakistan Journal of Chemistry

Microorganisms have numerous biotechnological applications for bioremediation of metal-contaminat... more Microorganisms have numerous biotechnological applications for bioremediation of metal-contaminated soil and water systems. In view of this, the present study was aimed to evaluate the abilities of microorganisms for tolerance and biosorption of heavy metals. In this study we have isolated 30 different bacterial strains from soil. Morphological and biochemical characterization was carried out. It was shown that Pseudomonas sp. was most common isolate among heavy metal resistant organisms that are capable of resisting 5 heavy metals (Ni, Pb, Cr, Cu and Cd) of concentration range 50-300µg/ml. Among 30 isolates, 4 of them showed high degree of resistance against all metals at concentration at 300µg/ml. Bacterial strains isolated in this study can be used in bioremediation and bio-mining.

Pakistan Journal of Chemistry

The present work focused on the production of extracellular lipase from Pseudomonas aeruginosa HE... more The present work focused on the production of extracellular lipase from Pseudomonas aeruginosa HE05 emplying natural oils of plants as substrat. Nine isolates (HE01-HE09) were isolated from the desiel oil contaminated soil and screened for lipase activity on nutrient agar medium supplemented with 1% olive oil and tween 80. Pseudomonas aeruginosa HE05 was identified as the hyperproducer of lipase. Maximum lipase production (3123U/ml) was recorded when 1% olive oil and 1% tween 80 added in nutrient broth used as a substrate in submerged fermentation. The optimal production was achevied in 96 hrs at 37 o C at 100 rpm. Quantification of lipase units were performed by turbidimetric assay. Supplementation of nutrient broth with natural oils was found as inducer as well as substrate for the production of lipase. Among the natural oils, mustard oil, corn oil, almond oil are better substrate for extracellular lipase production.

Glutamate decarboxylase (GAD) was isolated from a cell-free extract of E. coli JM109.and partiall... more Glutamate decarboxylase (GAD) was isolated from a cell-free extract of E. coli JM109.and partially purified by applying crude intracellular extract on Sephadex G-100. The enzyme was eluted in the first peak of the column and it was purified 27%. First peak showed highest GABA production activity on TLC plates. Absorption spectrum showed 0.33mg/ml of protein.

JPMA. The Journal of the Pakistan Medical Association, 2021

OBJECTIVE The main objective of the study was to evaluate the health of University of Karachi stu... more OBJECTIVE The main objective of the study was to evaluate the health of University of Karachi students (aged 19 to 21) by observing the correlation of haemoglobin level with underweight BMI. METHODS In this study 151 students were enrolled from the Department of Biotechnology, University of Karachi, Sindh Pakistan. Blood samples were collected and Haemoglobin estimation was performed by Sahli's Haemoglobinometer. BMI of each student was calculated as per WHO criteria for South Asian. Results Out of 151 students, 60 (39.7%) students had anaemia, among which 41(27.1%) were showing grade 1 anaemia and 19(12.5%) were showing grade 2 anaemia. BMI results showed that 50 (33.1%) students were underweight whereas, 19(12.5%) students were overweight and 12( 7.9%) students were obese. Correlation showed that 34 (22.5%) students had anaemia with underweight BMI. CONCLUSIONS This study concluded that anaemia with underweight BMI is more prevalent among females especially adolescent girls. T...

Pakistan journal of pharmaceutical sciences, 2019

Staphylococcus aureus is a common skin colonizer as well as opportunistic pathogen causing seriou... more Staphylococcus aureus is a common skin colonizer as well as opportunistic pathogen causing serious diseases including bacteremia, endocarditis and a number of different infections. It has a unique ability to swiftly respond and develop resistance for every other antibiotic introduced against it. The prevalence of antibiotic resistant strains of S. aureus is increasing on an alarming rate, which not only restrains the treatment options but the economic deprivation sustained due to infections of this superbug are incomputable. In our study, antimicrobial resistance patterns for 13 different antibiotics were evaluated in non-duplicate isolates of MSSA and MRSA isolated from different clinical samples (i.e. urine, pus, HVS, blood, tissue, wound and ear swabs). Most cultures were identified as multi-drug resistant (MDR). The highest resistance was recorded against ampicillin and erythromycin (88% each), while resistances against oxacillin, fosfomycin, cefoxitin and ciprofloxacin were als...

AUSTRALIAN JOURNAL OF BASIC AND APPLIED SCIENCES

Glucoamylase (EC 3.2.1.3) is exo-enzyme of extraordinary significance for saccharification of sta... more Glucoamylase (EC 3.2.1.3) is exo-enzyme of extraordinary significance for saccharification of starchy materials and other related oligosaccharides. Glucoamylase successively hydrolyzes 1,4-alpha glycosidic bond from the non-reducing end of starch and other related polysaccharides and release free glucose, which then used as a feedstock for the process of fermentation (Gupta et al., 2003; Norouzian et al., 2006). Industrial production of glucoamylase has been performed by the fungi, Aspergillus niger (Wang et al., 2006). Glucoamylase has different applications in significant areas of food processing, animals feed, fermentation biotechnology, paper making, fabric texture, grain hydrolysis for the alcohol industry (Zambare, 2011). The production of glucoamylase from different Aspergillus species including Aspergillus niger, Aspergillus fumigatus,

Journal of Basic & Applied Sciences