emmanuel haruna | Kaduna State University (original) (raw)

emmanuel haruna

Phone: +601126896856
Address: Department of Biomedical Science, Faculty of Health Science, Universiti Kebangsaan Malaysia

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Papers by emmanuel haruna

Research paper thumbnail of Isolation and Partial Purification of Invertase and Invertase Inhibitor from Sweet Potato Tuber (Ipomoea batatas)

Research paper thumbnail of Effect of Immunization with Cysteine Protease from Phase-Separated Parasite Proteins on the Erythrocytic Stage Development of the Chloroquine-Resistant, Plasmodium berghei in BALB/C Mice

Journal of Bacteriology & Parasitology, 2011

Research paper thumbnail of Cysteine protease from the malaria parasite, Plasmodium berghei-purification and biochemical characterization

Journal of Cell and …, 2011

Plasmodium berghei was isolated from mice red blood cells and phase-separated by Triton X-100 tem... more Plasmodium berghei was isolated from mice red blood cells and phase-separated by Triton X-100 temperature-induced phase separation procedures. The enzyme cysteine protease was purified 5.33 fold with a recovery of 58%. SDS-PAGE analysis of the enzyme ...

Research paper thumbnail of Pathogenic mechanisms of Trypanosoma evansi infections

Research in Veterinary Science, 2012

Insect-borne diseases exact a high public health burden and have a devastating impact on livestoc... more Insect-borne diseases exact a high public health burden and have a devastating impact on livestock and agriculture. To date, control has proved to be exceedingly difficult. One such disease that has plagued sub-Saharan Africa is caused by the protozoan African trypanosomes (Trypanosoma species) and transmitted by tsetse flies (Diptera: Glossinidae). This presentation describes Trypanosoma evansi (T. evansi) which causes the disease known as trypanosomosis (Surra) or trypanosomiasis in which several attempts have being made to unravel the clinical pathogenic mechanisms in T. evansi infections, yielding various reports which have implicated hemolysis associated to decrease in life span of erythrocytes and extensive erythrophagocytosis being among those that enjoy prominence. T. evansi generates Adenosine Triphosphate (ATP) from glucose catabolism which is required for the parasite motility and survival. Oxidation of the erythrocytes induces oxidative stress due to free radical generation. Lipid peroxidation of the erythrocytes causes membrane injury, osmotic fragility and destruction of the red blood cell (RBC) making anemia a hallmark of the pathology of T. evansi infections.

Research paper thumbnail of Evaluation of In Vitro Activity of Essential Oils against Trypanosoma brucei brucei and Trypanosoma evansi

Journal of Parasitology Research, 2010

Essential oils (EOs) from Cymbopogon citratus (CC), Eucalyptus citriodora (EC), Eucalyptus camald... more Essential oils (EOs) from Cymbopogon citratus (CC), Eucalyptus citriodora (EC), Eucalyptus camaldulensis (ED), and Citrus sinensis (CS) were obtained by hydrodistillation process. The EOs were evaluated in vitro for activity against Trypanosoma brucei brucei (Tbb) and Trypanosoma evansi (T. evansi). The EOs were found to possess antitrypanosomal activity in vitro in a dose-dependent pattern in a short period of time. The drop in number of parasite over time was achieved doses of 0.4 g/ml, 0.2 g/mL, and 0.1 g/mL for all the EOs. The concentration of 0.4 g/mL CC was more potent at 3 minutes and 2 minutes for Tbb and T. evansi, respectively. The GC-MS analysis of the EOs revealed presence of Cyclobutane (96.09%) in CS, 6-octenal (77.11%) in EC, Eucalyptol (75%) in ED, and Citral (38.32%) in CC among several other organic compounds. The results are discussed in relation to trypanosome chemotherapy.

Research paper thumbnail of Isolation and Partial Purification of Invertase and Invertase Inhibitor from Sweet Potato Tuber (Ipomoea batatas)

Research paper thumbnail of Effect of Immunization with Cysteine Protease from Phase-Separated Parasite Proteins on the Erythrocytic Stage Development of the Chloroquine-Resistant, Plasmodium berghei in BALB/C Mice

Journal of Bacteriology & Parasitology, 2011

Research paper thumbnail of Cysteine protease from the malaria parasite, Plasmodium berghei-purification and biochemical characterization

Journal of Cell and …, 2011

Plasmodium berghei was isolated from mice red blood cells and phase-separated by Triton X-100 tem... more Plasmodium berghei was isolated from mice red blood cells and phase-separated by Triton X-100 temperature-induced phase separation procedures. The enzyme cysteine protease was purified 5.33 fold with a recovery of 58%. SDS-PAGE analysis of the enzyme ...

Research paper thumbnail of Pathogenic mechanisms of Trypanosoma evansi infections

Research in Veterinary Science, 2012

Insect-borne diseases exact a high public health burden and have a devastating impact on livestoc... more Insect-borne diseases exact a high public health burden and have a devastating impact on livestock and agriculture. To date, control has proved to be exceedingly difficult. One such disease that has plagued sub-Saharan Africa is caused by the protozoan African trypanosomes (Trypanosoma species) and transmitted by tsetse flies (Diptera: Glossinidae). This presentation describes Trypanosoma evansi (T. evansi) which causes the disease known as trypanosomosis (Surra) or trypanosomiasis in which several attempts have being made to unravel the clinical pathogenic mechanisms in T. evansi infections, yielding various reports which have implicated hemolysis associated to decrease in life span of erythrocytes and extensive erythrophagocytosis being among those that enjoy prominence. T. evansi generates Adenosine Triphosphate (ATP) from glucose catabolism which is required for the parasite motility and survival. Oxidation of the erythrocytes induces oxidative stress due to free radical generation. Lipid peroxidation of the erythrocytes causes membrane injury, osmotic fragility and destruction of the red blood cell (RBC) making anemia a hallmark of the pathology of T. evansi infections.

Research paper thumbnail of Evaluation of In Vitro Activity of Essential Oils against Trypanosoma brucei brucei and Trypanosoma evansi

Journal of Parasitology Research, 2010

Essential oils (EOs) from Cymbopogon citratus (CC), Eucalyptus citriodora (EC), Eucalyptus camald... more Essential oils (EOs) from Cymbopogon citratus (CC), Eucalyptus citriodora (EC), Eucalyptus camaldulensis (ED), and Citrus sinensis (CS) were obtained by hydrodistillation process. The EOs were evaluated in vitro for activity against Trypanosoma brucei brucei (Tbb) and Trypanosoma evansi (T. evansi). The EOs were found to possess antitrypanosomal activity in vitro in a dose-dependent pattern in a short period of time. The drop in number of parasite over time was achieved doses of 0.4 g/ml, 0.2 g/mL, and 0.1 g/mL for all the EOs. The concentration of 0.4 g/mL CC was more potent at 3 minutes and 2 minutes for Tbb and T. evansi, respectively. The GC-MS analysis of the EOs revealed presence of Cyclobutane (96.09%) in CS, 6-octenal (77.11%) in EC, Eucalyptol (75%) in ED, and Citral (38.32%) in CC among several other organic compounds. The results are discussed in relation to trypanosome chemotherapy.

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