Dr GUNJI VENKATESWARLU | Kaziranga University (original) (raw)

Papers by Dr GUNJI VENKATESWARLU

World journal of pharmaceutical research, 2013

Research Journal of Pharmacy and Technology

Introduction: Plants have a special place in the treatment of cancer. It is estimated that plant-... more Introduction: Plants have a special place in the treatment of cancer. It is estimated that plant-derived compounds constitute more than 50% of anticancer agents. In this present study I attempted an experiment to find out the anti-cancer activity of selected plants Limnophila repens and Argyeia cymosa by using SRB Assay. Material method: I have collected the plants, dried very well and extracted with methanol crude methanol extract of the both plants tested for its anti-cancer activity. Results and disscussionThe anticancer activity of methanolic extracts of Argyreia cymosa and Limnophila repens was determined by sulforhodamine B colorimetric assay. The results of the cytotoxicity of extracts from both plant extracts analysed and The Methanol extract of Limnophila repens extract showed comparable activity to the standard compound, i.e., Adriamycin on Ishikawa and SCC-29B cell lines, respectively. This extract showed TGI, and GI50 was 38.9 and <10 µg/ ml on Ishikawa cell lines and...

Turkish journal of pharmaceutical sciences, Jun 1, 2020

Amaç: Limnophila repens (MELR) metanol ekstresinin analjezik ve anti-enflamatuvar etkileri 200 ve... more Amaç: Limnophila repens (MELR) metanol ekstresinin analjezik ve anti-enflamatuvar etkileri 200 ve 400 mg/kg dozda değerlendirildi. Gereç ve Yöntemler: Karagenin ile indüklenen pence ödeminde MELR'nin anti-enflamatuvar etkisi araştırıldı ve analjezik aktivite merkezi ve periferik modellerle değerlendirildi. Bulgular: MELR, farklı dozlarda (200 ve 400 mg/kg) güçlü analjezik ve anti-enflamatuvar etki göstermiştir. Çalışma sonuçları, Limnophila'nın hem analjezik hem de anti-enflamatuvar olarak kullanımını doğrulamıştır. Güçlü anti-enflamatuvar ve analjezik etkilerden anabolik steroidlerin (β-sitosterol ve stigmasterol gibi); flavonoidlerin (kuersetin ve glikozitler gibi) sorumlu olabileceği değerlendirilmiştir. Sonuç: Çalışmaya dayanarak Limnophila repens'in analjezik ve anti-enflamatuvar aktiviteye sahip olduğu sonucuna varabiliriz. Bununla birlikte, organik çalışmalara ek olarak, antinosiseptif ve anti-enflamatuvar etkilerden sorumlu ekstra enerjik kimyasalları değerlendirmek için ek fitokimyasallar gereklidir. Anahtar kelimeler: Limnophila repens, karagenin, fitokimyasal tarama, β-sitosterol Objectives: The analgesic and anti-inflammatory effects of methanolic extract of Limnophila repens (MELR) were assessed at 200 and 400 mg/kg. Materials and Methods: In carrageenan-mediated paw edema, the anti-inflammatory effect of MELR was investigated and analgesic activity was assessed by central and peripheral models Results: MELR had strong analgesic and anti-inflammatory effects at different dosages (200 and 400 mg/kg). The study results confirmed the use of Limnophila as both an analgesic and anti-inflammatory. The strong anti-inflammatory and analgesic effects can be caused by anabolic steroids, i.e. β-sitosterol and stigmasterol; and flavonoids, i.e. quercetin and glycosides, in the extraction of some kind of inflamed arbitrators. Conclusion: Based on the study, we can conclude that Limnophila repens had analgesic and anti-inflammatory activity. In addition to organic studies, however, additional phytochemicals are required to evaluate the extra energetic chemicals responsible for the antinociceptive and antiinflammatory effects.

Pharmacognosy Journal, 2019

Introduction: In the recent years there has been rapid growth in the field of herbal medicine mos... more Introduction: In the recent years there has been rapid growth in the field of herbal medicine most of the tradition systems of medicine are accepted universally after standardization only. it very important to develop an essential techniques to standardization of herbal related drugs. The present study standardization of Triphala Churna majorly focused on that area under WHO guidelines. Methods: This polyherbal Churna used treat the constipation and other gastric disorders. In this study a prepared Thriphala Churna was comparatively standardized with the reference obtained from market. For the standardization of the above formulations were done by evaluating the macroscopical, microscopical, powder flow properties, extractive values, Physicochemical characters, heavy metal content detection, qualitative and quantitive determination of tannins and alkaloids, TLC finger print, in-vitro anti-oxidant activity and cytotoxic activity to assess the quality and safety and therapeutic activity of formulation. Results: The above parameters for the both formulation complies with the strands. The flow properties are poor. From the preliminary phytochemical test revealed the presence of various bioactive constituents. Majorly the concentration of tannins and flavonoids are high in water extract and also the water extract having the good anti-oxidant and in vitro cytotoxic activity. Hence the Triphala extracts may be used for various Ayurvedic preparations to chronic diseases like cancer.

The present study was undertaken to investigate the protective effect and possible mechanism of m... more The present study was undertaken to investigate the protective effect and possible mechanism of methananolic extract of Barleria Montana Nees (BM) on paracetamol indued rat. This respective activity was assessed through monitoring liver function tests through the measurement of triglycerides, cholesterol, total protein, total bilirubin, serum enzymes like SGOT and SGPT and in vivo antioxidant parameters like lipid peroxidase, Superoxide dismutase (SOD) and catalase. Further hepatic tissues were also subjected to histopathological studies. Pretreatment of BM methanolic extract (500mg/kg) reduced the fatty liver symptoms and significantly (p<0.001) inhibited the increase of respective serum enzyme levels. The results of the present study indicated that BM methanolic extract possess Hepato protective effects which could act as an effective treatment for acute hepatic diseases.

The main objective of present research work is to formulate the of Domperidone Maleate floating t... more The main objective of present research work is to formulate the of Domperidone Maleate floating tablets. Domperidone Maleate, an antiemetic and a prokinetic agent belongs to BCS Class-II and Indicated for treatment of upper gastrointestinal motility disorders by blocking the action of Dopamine. The Floating tablets of Domperidone Maleate were prepared employing different concentrations of HPMCK4M and Guar Gum in different combinations as a release rate modifiers by Direct Compression technique using 32 factorial design. The concentration of HPMCK4M and Guar Gum was selected as independent variables, X1 and X2 respectively whereas, time required for drug dissolution t10%, t50%,t75%,t90%were selected as dependent variables. Totally nine formulations were designed and are evaluated for hardness, friability, thickness, Assay, Floating Lag time, In-vitro drug release. From the Results concluded that all the formulation were found to be with in the Pharmacopoeial limits and the In-vitro d...

Journal of Magnetism and Magnetic Materials, 2009

A nanosuspension of magnetically tagged metronidazole was developed by the solvent displacement m... more A nanosuspension of magnetically tagged metronidazole was developed by the solvent displacement method coupled with ultrasonication and was evaluated for its physicochemical properties. The drug release from metronidazole magnetic nanosuspension at pH 1.2 and 7.0 shows maximum correlation coefficient for zero order and Higuchi model, respectively. The anthelmintic activity of the formulated metronidazole magnetic nanosuspension was evaluated on Indian earthworms

Objective: The aim of the study is to evaluate the effect of the Hydroalcoholic extract of Moring... more Objective: The aim of the study is to evaluate the effect of the Hydroalcoholic extract of Moringa oleifera leaves on fertility hormone and semen quality of male albino rats. Materials and method: The Hydroalcoholic extract of Moringa oleiferaseed at doses of 100, 200 and 400 mg/kg were administrated for 30 days. The effect of the extract on body weight and sexual organs weights (testes and epididymis) were determined. The fertility hormone and semen characteristic was studied. Resultsand and discussion: Oral administration of Hydro alcoholic extract at doses of 100, 200 and 400 mg/kg were significantly increased body weight and sexual organ weight. Also significantly increased serum Testosterone, Follicle-stimulating hormone (FSH) and Luteinizing hormone (LH) compared to the control group, in addition, significantly increased semen characteristic in experimental animals study. Conclusion :The results of the present study demonstrate the effectiveness of Moringa oleiferaseed extract...

The leaves of Anchomanes difformis are used in traditional medicine to promote health in the agei... more The leaves of Anchomanes difformis are used in traditional medicine to promote health in the ageing. This study investigated the antidiabetic activity of the ethanol extract of the leaves of Anchomanes difformis in albino rats. Diabetes was induced by intra-peritoneal injection of 160 mg/kg of alloxan monohydrate. Plant extract (250 mg/kg and 500 mg/kg) was administered orally and blood glucose levels were monitored at 2 hourly intervals and daily for 14 days. The LD50 was found to be greater than 5000 mg/kg. Phytochemical screening showed the presence of tannins, phlobatannins and alkaloids, flavonoids, reducing sugars, saponins and cardiac glycosides. The two doses of the extract produced a statistically significant reduction of fasting blood glucose levels during the acute study and prolonged treatment for 14 days when compared to glibenclamide and the negative controls. The plant extract seems to possess a dosedependent anti diabetic effect. Further studies will be done to evaluate the toxicological effects of this plant extract.

International Journal of Green Pharmacy, 2019

Aim: The present work was performed to assess the hepatoprotective activity of Limnophila repens ... more Aim: The present work was performed to assess the hepatoprotective activity of Limnophila repens against paracetamol toxicity in Wistar rats. Materials and Methods: Hepatoprotective properties of the methanol extract of the whole plant had been examined on paracetamol-induced hepatotoxicity. Hepatotoxicity was evoked in albino Wistar rats by the administration of paracetamol (2 g/kg), p.o. for 7 days. The methanol extract of L. repens was administered at the doses 100–200 mg/kg/day, p.o. for 7 days. Serum analysis was performed to estimate the levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, albumin, total bilirubin, cholesterol, and proteins. Histopathology studies were worked on the catalase liver samples. Results: The noxious effects of paracetamol had been considerably controlled in the extract treated groups that were demonstrated by the restoration of serum biochemical parameters to near normal levels. Conclusions: From the research, it had...

The aim of the present study is to evaluate the effect of in-vitro anticancer activity of the pet... more The aim of the present study is to evaluate the effect of in-vitro anticancer activity of the petroleum ether extract of Cynodon dactylon against HEP-2 laryngeal, HELA cervical and MCF-7 breast cancer cell lines and it was compared with normal, Vero cell line using MTT assay showed a percentage of cell viability of 97% at 0.078 mg/ml which decrease with increase in concentration of extract. Anticancer activity of petroleum ether extract of Cynodon dactylon on HEP-2, HELA and MCF-7 cancer cell lines showed potent cytotoxic activity. The inhibition percentage with regard to cytotoxicity was found to be 93.5%, 88.5% and 79.2% at 10 mg/ml, which was comparable to the control Cyclophosphamide that showed a cytotoxicity of 96%, 92% and 83%. Therefore the minimum effective concentration of petroleum ether extract of Cynodon dactylon was non-toxic to Vero cells but toxic to HEP-2, HELA and MCF-7 cells(Ic50) was recorded at a concentration of 0.156 mg/ml 0.625 mg/ml of petroleum ether extrac...

To evaluate in vitro anticancer activity on the MCF-7 cell line (Human breast cancer cell line) o... more To evaluate in vitro anticancer activity on the MCF-7 cell line (Human breast cancer cell line) of Albizia saman (Leguminosae: family) flower extract. The monolayer cells were detached with trypsin-ethylene diamine tetra acetic acid (EDTA) to make single cell suspension and viable cells were counted using a hemocytometer. The cell suspension was diluted with medium containing 5% FBS (Fetal Blood Serum) to obtain final density of 1x105cells/ml. 100μl per well of cell suspension were seeded into 96-well plates at plating density of 10,000 cells/well and incubated to allow for cell attachment at 370C, 5% CO2, 95% air and 100% relative humidity. The results obtained from the in vitro studies performed using the human breast cancer cell line (MCF-7) reveals that the ethanolic extract of Albizia saman flower has a moderate anticancer activity with 94.72% growth inhibition at 200μg/ml. The IC50 value was 120.1μg/ml and the regression value was 0.999.

Asian Pacific Journal of Health Sciences, Oct 1, 2018

Background: In the present study we were evaluated pharmacognostical; antimicrobial and cytotoxic... more Background: In the present study we were evaluated pharmacognostical; antimicrobial and cytotoxic studies of the aqueous and Ethanolic extracts of the dietary polyherbal powders because they are great importance in the treatment of various metabolic disorder. Materials and methods: the following powder mixtures of Malus pumiplus (fruit), Momordica chirata (fruit) powder, Citrus limon (fruit), Pleurotus ostreatus (Mushroom), and Triticum aestivum (wheat grass) were extracted with distilled water and with ethanol. Results and conclusion: The result indicates that the above solvents having better extractive values with good bioactive components like Polyphenols, Glycosides, and Saponins. These are responsible bioactive phytochemical used in the herbal therapy. The aqueous and the Ethanolic were evaluated for The anti-microbial activity by cup plate method and the diameter of zone of inhibition is nearly equal to the standard drug and both the extracts showed potential cytotoxic activity through the in-vitro cytotoxic studies by MTT assay.

The main objective of present research work is to formulate the floating tablets of Carvedilol Ph... more The main objective of present research work is to formulate the floating tablets of Carvedilol Phosphate using 3 2 factorial design. Carvedilol Phosphate, non-selective α 1-β 1-blocking agent belongs to BCS Class-II and Indicated for treatment of Hypertension/moderate Heart Failure. The Floating tablets of Carvedilol Phosphate were prepared employing different concentrations of HPMCK100M and Sodium bicarbonate in different combinations by Direct Compression technique using 3 2 factorial design. The concentration of HPMCK100M and Sodium bicarbonate required to achieve desired drug release was selected as independent variables, X 1 and X 2 respectively whereas, time required for 10% of drug dissolution (t 10%), 50% (t 50%), 75% (t 75%) and 90% (t 90%) were selected as dependent variables. Totally nine formulations were designed and are evaluated for hardness, friability, thickness, % drug content, Floating Lag time, In-vitro drug release. From the Results concluded that all the formulation were found to be with in the Pharmacopoeial limits and the In-vitro dissolution profiles of all formulations were fitted in to different Kinetic models, the statistical parameters like intercept (a), slope (b) & regression coefficient (r) were calculated. Polynomial equations were developed for t 10% , t 50% , t 75% , t 90%. Validity of developed polynomial equations were verified by designing 2 check point formulations (C 1 , C 2). According to SUPAC guidelines the formulation (F 8) containing combination of 25% HPMCK100M and 3.75% Sodium bicarbonate, is the most similar formulation (similarity factor f 2 =88.801, dissimilarity factor f 1 = 2.250 & No significant difference, t= 0.095) to marketed product (CARDIVAS). The selected formulation (F 8) follows Higuchi's kinetics, and the mechanism of drug release was found to be Non-Fickian Diffusion (n= 1.035, Super Case-II transport).

The main objective of the present study is to formulate and evaluate a poly herbal ointment with ... more The main objective of the present study is to formulate and evaluate a poly herbal ointment with antiseptic activity.
Ointments were formulated using methanolic extracts of Eclipta alba, Ocimum sanctum, Azadiracta indica and Achyranthes
aspera which were evaluated for its physicochemical property, antibacterial and antioxidant activity. Ointments were
prepared using different concentrations of the extracts such as 2%, 4%, 6% w/w by fusion method using emulsifying
ointment as base. Formulations were then tested for its physicochemical properties which gave satisfactory results. The
prepared formulations were also stable at 4ºC, 25ºC and 37ºC. Further, Polyherbal formulations were evaluated for its anti- bacterial activity against Betadine (5%w/w) as the standard. All the formulations showed Predominant activity against
selected species. Formulations were also evaluated for anti-oxidant activity through reducing power assay, nitric oxide and
hydrogen peroxide scavenging method. The results showed that the scavenging activity of the formulations increased with
increase in concentration and this is due to the presence of flavanoids and tannins. The presence of both antibacterial and
antioxidant activity reveals that the prepared ointment can also be used for wound healing. Hence an attempt was made to
formulate a Polyherbal ointment, and to evaluate for its physical parameter, in-vitro anti-oxidant activity and to compare its
antibacterial activity with a marketed formulation (5% w/w Betadine).Overall result of this study reveals that this is an
effective Polyherbal antiseptic ointment.
Keywords: Eclipta alba, Ocimum sanctum, Azadiracta indica, Achyranthes aspera Formulations, Spread ability,
Extrudability