Balasaheb Gaykar | Savitribai Phule University of Pune (original) (raw)
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Papers by Balasaheb Gaykar
Indo Global Journal of Pharmaceutical Sciences, 2019
Indo Global Journal of Pharmaceutical Sciences, 2018
Advances in Plant Sciences, 2013
Some plants are used in folk medicine for prevention of pregnancy. The study aimed to assess the ... more Some plants are used in folk medicine for prevention of pregnancy. The study aimed to assess the acute toxicity of synergistic formulation of traditional contraceptive plants. Acute toxicity means adverse change occurring immediately on exposure to a substance. Acute toxicity of crude petroleum and aqueous extract was evaluated by oral route administration to female mice by using OECD-423 guidelines. It was a single high extract dose of 2000mg/kg administered and the effects on mortality, behavioral pattern as well as spontaneous locomotors activity were evaluated. The limit dose of 2000 mg/kg did not cause any mortality. The findings suggested that the LD50 value of aqueous and petroleum ether was found to be greater than 2000 mg/kg. Hence, synergistic extract of plants is nontoxic. This work is useful to find out the dose structure for further experimental work on evaluation of traditional contraceptive plants and their activity
Asian Journal of Applied Science and Technology, 2021
Journal of Tropical Life Science, 2019
nebio.in
... Gaykar BM, Sonali Thombre and Sudhir Suryawanshi Post Graduate Department of Botany ... The m... more ... Gaykar BM, Sonali Thombre and Sudhir Suryawanshi Post Graduate Department of Botany ... The microscopic photographs were taken with Sony Digital 5.1 pixel camera and ... Acknowledgment We are thankful to Dr. RJ Barnabus, Principal, Ahmednagar College, Ahmednagar for ...
Asian Journal of Applied Science and Technology, 2020
Journal of Medicinal Plants Studies, 2017
Moringa oleifeira Lam is common Indian plant belongs to monogeneric Moringaceae. The research inv... more Moringa oleifeira Lam is common Indian plant belongs to monogeneric Moringaceae. The research investigated the presence etynyl etradiol in ‘Drumstick’ (Moringa oleifera Lam.) seed samples. The major compounds found in Moringa oleifera Lam. seeds were further analyzed by HPLC technique. High pressure liquid chromatography (HPLC) has been found to be an efficient and sensitive method for identification and quantification of different classes of Ethynylestradiol. HPLC studies showed that analysis of Ethynylestradiol derivatives extracted in petroleum ether and aqueous seed extracts of Moringa oleifera Lam. revealed significantly high levels. The calculated value of ethynyl estradiol was obtained 108.199 μg/ml of petroleum extract while it was obtained 655.26 μg/ml of Moringa oleifera aqueous extract. As the concentration of the samples increased, the peak area was also increased.
Purpose: Quantitative analysis of steroidal saponin “Diosgenin” from methanol extract of Moringa ... more Purpose: Quantitative analysis of steroidal saponin “Diosgenin” from methanol extract of Moringa oleifera Lam (Drumstick) by HPTLC technique. Approach: The approach of the study was to find out the presence of Diosgenin from Moringa and then its quantity. Method: HPTLC analysis was performed with methonolic extract of seeds. Standard Diosgenin curve was used to detect the availability of the steroidal saponin Diosgenin. Results: 20 µL of sample was applied which showed 159.4084 ng of Diosgenin. About 0.797 % w/v of Diosgenin was estimated form seeds of Moringa in methonolic extract. It was compared with the standard Diosgenin curve. Conclusions: Moringa oleifera Lam seeds are the source of steroidal saponin-Diosgenin which can be used as female contraceptive to control human population.
Asian Journal of Applied Science and Technology (AJAST), 2021
The plant growth promoting rhizobacterias inoculants used in various formulations for different p... more The plant growth promoting rhizobacterias inoculants used in various formulations for different purpose. In addition to rhizobacterias, the formulation may also contain various additives. Furthermore, it is important to understand the interaction between bacteria and formulation materials. The formulation materials serve as cell protectants which enhance the shelf-life of bacteria. It is proved that the Rhizobium is the effective inoculants along with specific nutrient media for survival. Many researchers have shown that liquid rhizobial formulations are more beneficial than solid bio-fertilizer formulations. In the present work the survival of Azotobacter in liquid formulations were evaluated by using Glycerol at different concentration in Jenson’s broth. It was noted that survival of Azotobacter was concentration correlated. Lowest number of colonies in 5 mM in the medium containing glycerol (23 colonies at zero days and 5 colonies on 360th day) and highest number of colonies in 2...
Indo Global Journal of Pharmaceutical Sciences, 2019
Purpose: Quantitative analysis of steroidal saponin „Diosgenin‟ from methanol extract of Moringa ... more Purpose: Quantitative analysis of steroidal saponin „Diosgenin‟ from methanol extract of Moringa oleifera Lam (Drumstick) by HPTLC technique. Approach: The approach of the study was to find out the presence of Diosgenin from Moringa and then its quantity. Method: HPTLC analysis was performed with methonolic extract of seeds. Standard Diosgenin curve was used to detect the availability of the steroidal saponin Diosgenin. Results: 20 µL of sample was applied which showed 159.4084 ng of Diosgenin. About 0.797 % w/v of Diosgenin was estimated form seeds of Moringa in methonolic extract. It was compared with the standard Diosgenin curve. Conclusions: Moringa oleifera Lam seeds are the source of steroidal saponin- Diosgenin which can be used as female contraceptive to control human population. Keywords: Contraceptive, Diosgenin, HPTLC and Moringa oleifera Lam.
Moringa oleifeira Lam is common Indian plant belongs to monogeneric Moringaceae. The research inv... more Moringa oleifeira Lam is common Indian plant belongs to monogeneric Moringaceae. The research investigated the presence etynyl etradiol in 'Drumstick' (Moringa oleifera Lam.) seed samples. The major compounds found in Moringa oleifera Lam. seeds were further analyzed by HPLC technique. High pressure liquid chromatography (HPLC) has been found to be an efficient and sensitive method for identification and quantification of different classes of Ethynylestradiol. HPLC studies showed that analysis of Ethynylestradiol derivatives extracted in petroleum ether and aqueous seed extracts of Moringa oleifera Lam. revealed significantly high levels. The calculated value of ethynyl estradiol was obtained 108.199 µg/ml of petroleum extract while it was obtained 655.26 µg/ml of Moringa oleifera aqueous extract. As the concentration of the samples increased, the peak area was also increased.
Some plants are used in folk medicine for prevention of pregnancy. The study aimed to assess the ... more Some plants are used in folk medicine for prevention of pregnancy. The study aimed to assess the acute toxicity of synergistic formulation of traditional contraceptive plants. Acute toxicity means adverse change occurring immediately on exposure to a substance. Acute toxicity of crude petroleum and aqueous extract was evaluated by oral route administration to female mice by using OECD-423 guidelines. It was a single high extract dose of 2000mg/kg administered and the effects on mortality, behavioral pattern as well as spontaneous locomotors activity were evaluated. The limit dose of 2000 mg/kg did not cause any mortality. The findings suggested that the LD50 value of aqueous and petroleum ether was found to be greater than 2000 mg/kg. Hence, synergistic extract of plants is nontoxic. This work is useful to find out the dose structure for further experimental work on evaluation of traditional contraceptive plants and their activity.
Purpose: Quantitative analysis of steroidal saponin „Diosgenin‟ from methanol extract of Moringa ... more Purpose: Quantitative analysis of steroidal saponin „Diosgenin‟ from methanol extract of Moringa oleifera Lam (Drumstick) by HPTLC technique.
Approach: The approach of the study was to find out the presence of Diosgenin from Moringa and then its quantity.
Method: HPTLC analysis was performed with methonolic extract of seeds. Standard Diosgenin curve was used to detect the availability of the steroidal saponin Diosgenin.
Results: 20 μL of sample was applied which showed 159.4084 ng of Diosgenin. About 0.797 % w/v of Diosgenin was estimated form seeds of Moringa in methonolic extract. It was compared with the standard Diosgenin curve.
Conclusions: Moringa oleifera Lam seeds are the source of steroidal saponin- Diosgenin which can be used as female contraceptive to control human population.
The morphological nature of pollinarium of Cryptostegia, Cryptolepis and Hemidesmus are different... more The morphological nature of pollinarium of Cryptostegia, Cryptolepis and Hemidesmus are different from rest of the members of Asclepiadaceae. Maximum size was measured in Cryptolepis and it is 71.2 x 5.34 (L x B) m. In Cryptostegia the size of pollinarium is 4.25 x 1.87 m. Both the structures are spoon shaped shows single bag of pollen grain mass. Due to this variation, these taxa were classified under its tribe Periplocoidae. In the members of family Asclepiadaceae the size of pollinarium was ranges from 3.57 x 1.53 m (Asclepias curassavia L.) to 71.2 x 44.5 m (Gampho-carpus physocarpus E. Mey. Comm.). In rest of the members the size was variable as well as their structures were different. Maximum length of translator was found in Gymnema sylvestre (Retz) R. Br. (48.06 m.), while it was 0.85 m in Calotropis procera (L.) Ait. R. Br.
Twenty four taxa belonging to the family Euglenophyceae have been recorded from the fresh water b... more Twenty four taxa belonging to the family Euglenophyceae have been recorded from the fresh water bodies in eight sites of Ahmednagar District, Maharashtra. In this paper, Euglena, Phacus, Lepocinclis, Colacium are reported for the first time from the region (Pimpalgaon malvi-talav, Kapurwadi-talav, Dongargan sacred grove stream, and Sina river).
Indo Global Journal of Pharmaceutical Sciences, 2019
Indo Global Journal of Pharmaceutical Sciences, 2018
Advances in Plant Sciences, 2013
Some plants are used in folk medicine for prevention of pregnancy. The study aimed to assess the ... more Some plants are used in folk medicine for prevention of pregnancy. The study aimed to assess the acute toxicity of synergistic formulation of traditional contraceptive plants. Acute toxicity means adverse change occurring immediately on exposure to a substance. Acute toxicity of crude petroleum and aqueous extract was evaluated by oral route administration to female mice by using OECD-423 guidelines. It was a single high extract dose of 2000mg/kg administered and the effects on mortality, behavioral pattern as well as spontaneous locomotors activity were evaluated. The limit dose of 2000 mg/kg did not cause any mortality. The findings suggested that the LD50 value of aqueous and petroleum ether was found to be greater than 2000 mg/kg. Hence, synergistic extract of plants is nontoxic. This work is useful to find out the dose structure for further experimental work on evaluation of traditional contraceptive plants and their activity
Asian Journal of Applied Science and Technology, 2021
Journal of Tropical Life Science, 2019
nebio.in
... Gaykar BM, Sonali Thombre and Sudhir Suryawanshi Post Graduate Department of Botany ... The m... more ... Gaykar BM, Sonali Thombre and Sudhir Suryawanshi Post Graduate Department of Botany ... The microscopic photographs were taken with Sony Digital 5.1 pixel camera and ... Acknowledgment We are thankful to Dr. RJ Barnabus, Principal, Ahmednagar College, Ahmednagar for ...
Asian Journal of Applied Science and Technology, 2020
Journal of Medicinal Plants Studies, 2017
Moringa oleifeira Lam is common Indian plant belongs to monogeneric Moringaceae. The research inv... more Moringa oleifeira Lam is common Indian plant belongs to monogeneric Moringaceae. The research investigated the presence etynyl etradiol in ‘Drumstick’ (Moringa oleifera Lam.) seed samples. The major compounds found in Moringa oleifera Lam. seeds were further analyzed by HPLC technique. High pressure liquid chromatography (HPLC) has been found to be an efficient and sensitive method for identification and quantification of different classes of Ethynylestradiol. HPLC studies showed that analysis of Ethynylestradiol derivatives extracted in petroleum ether and aqueous seed extracts of Moringa oleifera Lam. revealed significantly high levels. The calculated value of ethynyl estradiol was obtained 108.199 μg/ml of petroleum extract while it was obtained 655.26 μg/ml of Moringa oleifera aqueous extract. As the concentration of the samples increased, the peak area was also increased.
Purpose: Quantitative analysis of steroidal saponin “Diosgenin” from methanol extract of Moringa ... more Purpose: Quantitative analysis of steroidal saponin “Diosgenin” from methanol extract of Moringa oleifera Lam (Drumstick) by HPTLC technique. Approach: The approach of the study was to find out the presence of Diosgenin from Moringa and then its quantity. Method: HPTLC analysis was performed with methonolic extract of seeds. Standard Diosgenin curve was used to detect the availability of the steroidal saponin Diosgenin. Results: 20 µL of sample was applied which showed 159.4084 ng of Diosgenin. About 0.797 % w/v of Diosgenin was estimated form seeds of Moringa in methonolic extract. It was compared with the standard Diosgenin curve. Conclusions: Moringa oleifera Lam seeds are the source of steroidal saponin-Diosgenin which can be used as female contraceptive to control human population.
Asian Journal of Applied Science and Technology (AJAST), 2021
The plant growth promoting rhizobacterias inoculants used in various formulations for different p... more The plant growth promoting rhizobacterias inoculants used in various formulations for different purpose. In addition to rhizobacterias, the formulation may also contain various additives. Furthermore, it is important to understand the interaction between bacteria and formulation materials. The formulation materials serve as cell protectants which enhance the shelf-life of bacteria. It is proved that the Rhizobium is the effective inoculants along with specific nutrient media for survival. Many researchers have shown that liquid rhizobial formulations are more beneficial than solid bio-fertilizer formulations. In the present work the survival of Azotobacter in liquid formulations were evaluated by using Glycerol at different concentration in Jenson’s broth. It was noted that survival of Azotobacter was concentration correlated. Lowest number of colonies in 5 mM in the medium containing glycerol (23 colonies at zero days and 5 colonies on 360th day) and highest number of colonies in 2...
Indo Global Journal of Pharmaceutical Sciences, 2019
Purpose: Quantitative analysis of steroidal saponin „Diosgenin‟ from methanol extract of Moringa ... more Purpose: Quantitative analysis of steroidal saponin „Diosgenin‟ from methanol extract of Moringa oleifera Lam (Drumstick) by HPTLC technique. Approach: The approach of the study was to find out the presence of Diosgenin from Moringa and then its quantity. Method: HPTLC analysis was performed with methonolic extract of seeds. Standard Diosgenin curve was used to detect the availability of the steroidal saponin Diosgenin. Results: 20 µL of sample was applied which showed 159.4084 ng of Diosgenin. About 0.797 % w/v of Diosgenin was estimated form seeds of Moringa in methonolic extract. It was compared with the standard Diosgenin curve. Conclusions: Moringa oleifera Lam seeds are the source of steroidal saponin- Diosgenin which can be used as female contraceptive to control human population. Keywords: Contraceptive, Diosgenin, HPTLC and Moringa oleifera Lam.
Moringa oleifeira Lam is common Indian plant belongs to monogeneric Moringaceae. The research inv... more Moringa oleifeira Lam is common Indian plant belongs to monogeneric Moringaceae. The research investigated the presence etynyl etradiol in 'Drumstick' (Moringa oleifera Lam.) seed samples. The major compounds found in Moringa oleifera Lam. seeds were further analyzed by HPLC technique. High pressure liquid chromatography (HPLC) has been found to be an efficient and sensitive method for identification and quantification of different classes of Ethynylestradiol. HPLC studies showed that analysis of Ethynylestradiol derivatives extracted in petroleum ether and aqueous seed extracts of Moringa oleifera Lam. revealed significantly high levels. The calculated value of ethynyl estradiol was obtained 108.199 µg/ml of petroleum extract while it was obtained 655.26 µg/ml of Moringa oleifera aqueous extract. As the concentration of the samples increased, the peak area was also increased.
Some plants are used in folk medicine for prevention of pregnancy. The study aimed to assess the ... more Some plants are used in folk medicine for prevention of pregnancy. The study aimed to assess the acute toxicity of synergistic formulation of traditional contraceptive plants. Acute toxicity means adverse change occurring immediately on exposure to a substance. Acute toxicity of crude petroleum and aqueous extract was evaluated by oral route administration to female mice by using OECD-423 guidelines. It was a single high extract dose of 2000mg/kg administered and the effects on mortality, behavioral pattern as well as spontaneous locomotors activity were evaluated. The limit dose of 2000 mg/kg did not cause any mortality. The findings suggested that the LD50 value of aqueous and petroleum ether was found to be greater than 2000 mg/kg. Hence, synergistic extract of plants is nontoxic. This work is useful to find out the dose structure for further experimental work on evaluation of traditional contraceptive plants and their activity.
Purpose: Quantitative analysis of steroidal saponin „Diosgenin‟ from methanol extract of Moringa ... more Purpose: Quantitative analysis of steroidal saponin „Diosgenin‟ from methanol extract of Moringa oleifera Lam (Drumstick) by HPTLC technique.
Approach: The approach of the study was to find out the presence of Diosgenin from Moringa and then its quantity.
Method: HPTLC analysis was performed with methonolic extract of seeds. Standard Diosgenin curve was used to detect the availability of the steroidal saponin Diosgenin.
Results: 20 μL of sample was applied which showed 159.4084 ng of Diosgenin. About 0.797 % w/v of Diosgenin was estimated form seeds of Moringa in methonolic extract. It was compared with the standard Diosgenin curve.
Conclusions: Moringa oleifera Lam seeds are the source of steroidal saponin- Diosgenin which can be used as female contraceptive to control human population.
The morphological nature of pollinarium of Cryptostegia, Cryptolepis and Hemidesmus are different... more The morphological nature of pollinarium of Cryptostegia, Cryptolepis and Hemidesmus are different from rest of the members of Asclepiadaceae. Maximum size was measured in Cryptolepis and it is 71.2 x 5.34 (L x B) m. In Cryptostegia the size of pollinarium is 4.25 x 1.87 m. Both the structures are spoon shaped shows single bag of pollen grain mass. Due to this variation, these taxa were classified under its tribe Periplocoidae. In the members of family Asclepiadaceae the size of pollinarium was ranges from 3.57 x 1.53 m (Asclepias curassavia L.) to 71.2 x 44.5 m (Gampho-carpus physocarpus E. Mey. Comm.). In rest of the members the size was variable as well as their structures were different. Maximum length of translator was found in Gymnema sylvestre (Retz) R. Br. (48.06 m.), while it was 0.85 m in Calotropis procera (L.) Ait. R. Br.
Twenty four taxa belonging to the family Euglenophyceae have been recorded from the fresh water b... more Twenty four taxa belonging to the family Euglenophyceae have been recorded from the fresh water bodies in eight sites of Ahmednagar District, Maharashtra. In this paper, Euglena, Phacus, Lepocinclis, Colacium are reported for the first time from the region (Pimpalgaon malvi-talav, Kapurwadi-talav, Dongargan sacred grove stream, and Sina river).