Sachin Tembhurne | Savitribai Phule University of Pune (original) (raw)

Papers by Sachin Tembhurne

Annals of Pharmacology and Pharmaceutics, Apr 12, 2017

The purpose of this study was to assess the safety use of Murraya koenigii in terms of repeated d... more The purpose of this study was to assess the safety use of Murraya koenigii in terms of repeated dose toxicity study in male and female rats. Three doses 300, 500 and 900 mg/kg of ethanolic extract of Murraya koenigii leaves were administered to groups of 10 animals (five male and five female) daily for 28 days respectively. Animals receiving the vehicle (0.5% CMC) served as a control. The biochemical parameters like RBC, WBC, glucose, hemoglobin, cholesterol, creatinine, bilurubin, SGPT and SGOT were analyzed in all groups of animals respectively. While in physiological parameters like daily food consumption, weekly body weight, visual, auditory and organ weights were analyzed respectively. At the end of study all the animals were sacrificed and tested for histopathology for any structural damage to major organs. Result of study showed that none of the animals from any dose levels showed test material related changes in RBC, WBC, creatinine, bilurubin, SGPT and SGOT parameter respec...

Essential oilhas been reported to have many pharmacological activities, one of which is their pro... more Essential oilhas been reported to have many pharmacological activities, one of which is their property to repel the mosquitoes and insects.The marigold infused oil is reported to be antibacterial, astringent, antifungal, used in insect bites etc.Mosquito repellent cream of different concentrations i.e. 5% and 7.5% , a combination of marigold oil with two essential oils i.e. clove oil and dill oil were formulated and evaluatedas described in the American Society for Testing and Materials (ASTM) standard E951-83 Laboratory testing of non-commercial mosquito repellent formulation on the skin.All formulations were subjected to sensory evaluation with respect to color, fragrance, appearance, shine and ease of applications as well as constant pH, clarity, homogeneityand spreadability.

Many drugs affect GI transit by acting as agonists or antagonists at specific cellular receptors,... more Many drugs affect GI transit by acting as agonists or antagonists at specific cellular receptors, such as cholinergic, adrenergic, serotonergic, opioidergic and calcium channels. Atropine is frequently used as a tool for identifying mechanisms involving cholinergic pathway in evaluation of GI transit time. The present study was aim at to evaluate the influence to Murraya koenigii on GI transit time through involvement of cholinergic systems/mechanisms. Ethanolic and petroleum ether extract of MKL at the doses of 300 and 500 mg/kg p.o. administered 15 hrs fasted Swiss albino male mice. 4% charcoal meal was administered (10ml/kg p.o.) 1 hr after the drug treatments and 20 min after all the animals were dissected for determination of the intestinal transit. For exploration of the cholinergic mechanisms atropine (1mg/kg i.p.) was administered 30 min prior administration of drug. The results of study indicate that MKL accelerate intestinal transit in normal mice. Atropine inhibits the in...

Dietary supplements Garlic and Black Sesame consist of phytoestrogens; it has been clinically use... more Dietary supplements Garlic and Black Sesame consist of phytoestrogens; it has been clinically used for treating mild depressive disorders by possible Estrogenic and Antioxidant mechanism. The purpose o f the study was to examine the effect of dietary suppleme nts on depression in ovariectomized (OVX) rat by de spair force swimming test and In vivo-lipid peroxidation study. After the treatment of G arlic 250 and 500 mg/kg and Black Sesame 500 mg/kg P.O. for three month in OVX rats, decrease the immobility period which was comp are to OVX control group rats (P<0.0001). Oxidative str e s is a measure of steady state level of reactive oxygen species (ROS) in the biological system. Treatment o f Garlic and Black Sesame can decrease the oxidativ e s ress in OVX rats. In present study lipid peroxidation wa s measured in terms of TBARS and result indicate to significant increase in blood serum TBARS content i n OVX control rats compared with Sham (SH) control rats (P<0.05). However in...

Common baldness, sometimes called male or female pattern baldness, accounts for 99 percent of hai... more Common baldness, sometimes called male or female pattern baldness, accounts for 99 percent of hair loss in men and women. Although its exact causes are unknown, heredity, hormones and age are contributing factors. The objective of the present work was to evaluate the influence of pig oil (Lard) on hair growth pattern in Wister rats. The hairs of one area of dorsal surface of rats removed by the application of hair removal cream and the pig oil (0.5 ml) were applied. Minoxidil (10%) was used as standard. The hair depilated regions were observed for the hair growth initiation afterward the length of hair was measured at 15th, 21st, 30th and 40th day. The result of present study indicates that the pig oil significantly potentiate to increase in hair growth pattern in rats. The significant effect of pig oil start to observed from 15th day. The results were comparatively more significant than standard Minoxidil. While the exact mechanism and chemical constituent responsible for the hair ...

Asian Journal of Pharmacy and Pharmacology, Oct 1, 2018

In this study, we tested the effect of hydroalcoholic extract of on oxidative stress, lipid Dalbe... more In this study, we tested the effect of hydroalcoholic extract of on oxidative stress, lipid Dalbergia sissoo profile in 90 days postsurgical bilateral ovariectomized female rats. 3-month old female Material and Methods: Wistar rats were used and distributed in 6 groups: intact groups with 90 days, ovariectomized groups with 90 days, ovariectomized (Ovx) treated hydroalcoholic extract of (200, 350 and 500mg/kg p.o.), Dalbergia sissoo ovariectomized treated with standard β Estradiol (0.1mg/kg/s.c). All the treatments were given for further 90 days after post-surgical period (90 days) in ovariectomized female rats. After 90 days of treatments serum estrogen level, level of thiobarbituric acid reactive substances and lipid profile were quantified. The treatment of the hydroalcoholic Results: extract of (200, 350 and 500mg/kg) in the Ovx rats shows significant increase in the serum estrogen Dalbergia sissoo level, normalization of lipid profile, decreased in the thiobarbituric acid reactive substances. All the results of Dalbergia sissoo are comparable with standard β Estradiol. Our results suggest that 90 days of treatment with Conclusion: hydroalcoholic extract of is able to increase the estrogen level thereby normalized the lipid profile and Dalbergia sissoo oxidative stress in ovariectomized rats.

World Journal of Pharmaceutical Research, 2017

Inflammation is defined as a sequence of events that occurs in response to noxious stimuli, infec... more Inflammation is defined as a sequence of events that occurs in response to noxious stimuli, infection or trauma. Symptoms of inflammations are local redness, swelling, pain, heat and loss of function. The present study was performed to evaluate the anti-inflammatory and wound healing activity of pyrazinamide and Clofazemine in Wistar rat. The aim of present work is to study the screening of synthetic compounds for its pharmacological aspects in inflammation and wound healing activity by using various preclinical models of wound healing and inflammation.The both drugs were studied for their anti-inflammatory activity by using carrageenan-induced paw edema in rats and the mean increase in paw volume in paw volume were measured by plethysmometer at different time intervals after carrageenan (1% w/v) injection. The pyrazinamide and Clofazemine were further evaluated for wound healing activity using incision wound model. The pyrazinamide and Clofazemine showed significant (p<0.05) reduction in the carrageenan-induced paw edema in rats and incision wound healing activity evidenced method in Wistar rats. The pyrazinamide and Clofazemine showed a greater antiinflammatory and wound healing effect when compared with the standard drugs, indomethacin and povidone iodine respectively. The present observation indicated significant (p<0.0001) activity of the pyrazinamide and Clofazemine in the treatment of inflammation and wound healing.

Der Pharma Chemica, Jan 12, 2015

Dalbergia sissoo Roxb. popularly known as Sisam in India belongs to the family Fabaceae. It is an... more Dalbergia sissoo Roxb. popularly known as Sisam in India belongs to the family Fabaceae. It is an important plant of great economic importance. The objective of the present work was to compare different extract for the in-vitro anthelmintic potency of Dalbergia sissoo Roxb. leaves using Indian earthworms (Pheretima posthumad). The various extracts of Dalbergia sissoo roxb. includes petroleum ether, carbon tetrachloride, benzene and ethanol were used in different concentrations viz. 10, 25, 50 and 100 mg/ml of respectively for in-vitro testing of anthelmintic potency by determination of time of paralysis and time of death of worm. Piperazine citrate was used as standard. The result of present study of indicates Dalbergia sissoo potentiate to paralyze earthworm and also caused its death after some time. The shortest time of paralysis was observed at 100 mg/ml of carbon tetrachloride extract of Dalbergia sissoo which was 19min 14 sec and 48 min 15sec respectively. The results of the study were comparable to standard Piperazine citrate. The results of the present study demonstrate that carbon tetrachloride extract of Dalbergia sissoo leaves took less time to cause paralysis of the earthworm than other three extracts. Thus the present study demonstrates the traditional claim of Dalbergia sissoo Roxb. as an anthelmintic has been confirm as the ethanolic, benzene, petroleum ether and carbon tetra chloride extracts of leaves displayed activity against the earthworm used in study. While carbon tetrachloride extract demonstrated to highly potent anthelmintic activity compared to other three extract of Dalbergia sissoo Roxb.

Int J PharmTech Res, 2010

... Beneficial Effects of Ethanolic Extract of Murraya Koenigii (Linn) Leaves in Cognitive Defici... more ... Beneficial Effects of Ethanolic Extract of Murraya Koenigii (Linn) Leaves in Cognitive Deficit Aged Mice Involving Possible ... 25. Gibson, GE, Duffy, TE, (1981), Impaired synthesis of acetylcholine by mild hypoxic hypoxia or nitrous oxide. J Neurochem., 36(1), 28–33. ...

Int J Pharm Tech Res, 2009

... 2. Prajapati, ND, Purohit, SS, Sharma, AK, & Kumar, T., (2003), A Handbook of Medicinal P... more ... 2. Prajapati, ND, Purohit, SS, Sharma, AK, & Kumar, T., (2003), A Handbook of Medicinal Plants (Agrobios, Jodhpur), pp. ... 25. Arulselvon, GP, Senthilkumar, D., Sathish Kumar., & Subramanian, S., (2006), Antidiabetic effect of Murraya koenigii leaves on streptozotocin induced ...

Int. J. Pharm. Technol. Res, 2009

... Louis Publishers), pp. 1198. 19. Jagtap, A., Tembhurne, S., Shirke, S., Patel, M.,(2006), Tox... more ... Louis Publishers), pp. 1198. 19. Jagtap, A., Tembhurne, S., Shirke, S., Patel, M.,(2006), Toxicological evaluation of Caralluma fimbriata extract in Wistar Rats. The American Abstract of Pharmaceutical Sciences., 8 S2, W4083. 20. ...

Indo American Journal of Pharmaceutical Research, 2013

international journal of veterinary science, 2015

Investigation of ethanolic and petroleum ether extracts from the Murraya koenigii fruits (MKF) fo... more Investigation of ethanolic and petroleum ether extracts from the Murraya koenigii fruits (MKF) for their anthelmintic activity against Indian earthworm (Pheretima posthuma). Four concentrations (50, 100, 150 and 200 mg/ml) of each extracts were studied in activity, which involved the determination of time of paralysis and time of death of the worm. Piperazine citrate (15 mg/ml) was taken as a reference standard drug and distilled water as control. When the dose of extract gradually increased Murraya Koenigii shows gradual increase in anthelmintic activity characterized by paralysis of earthworm and its death. The ethanolic extract showed better anthelmintic activity as compared with petroleum ether extract. From the results of the present study, it concluded that the ethanolic and petroleum ether extracts of Murraya koenigii fruit possesses anthelmintic activity. The present study is further supported by isolation of two carbazole alkaloids from fruit extract which may be responsibl...

Alzheimer’s disease (AD) is said to be the leading cause of dementia in elderly individuals exhib... more Alzheimer’s disease (AD) is said to be the leading cause of dementia in elderly individuals exhibit a marked decline in cognitive functions. There are several factors involves in the pathogenesis of AD e.g. involvement of cholinesterase enzyme, oxidative free radicals and high cholesterol level promoting risk for and exacerbating AD pathology. In the light of this, the present study was undertaken to investigate the influence of petroleum ether extracts (300 and 500mg/kg p.o.) of Murraya koenigii leaves (MKL) on memory in aged mice. The study was also undertaken to evaluate for cholinesterase and cholesterol level. The result of the present study indicates that administration of MKL for 15 days produce significant dose dependant improvement of memory. The results also indicate to reduce the brain cholinesterase activity and total cholesterol levels respectively. Thus it concludes that the underlying mechanism of action for the observed nootropic effect may be attributed to anticholi...

Journal of Medicinal Plants Research, 2010

Diabetes causes increased oxidative stress, which is thought to play an important role in the pat... more Diabetes causes increased oxidative stress, which is thought to play an important role in the pathogenesis of various diabetic complications. The antioxidant actions are keys to preventing or reversing diabetes and its complications. Thus the aim of the present study was to evaluate the antioxidant potential of ethanolic Murraya koenigii leaves (MKL) extract on Thiobarbituric Acid Reactive Substances (TBARS) as index of lipid peroxidation and on the glycemic control in streptozotocin induced diabetic rats. Effect of oral administration of MKL (300 and 500 mg/kg) on the level of blood glucose, glycosylated haemoglobin, on TBARS and glycogen levels were estimated in streptozotocin induced diabetic rats. Glibenclamide was used as a standard drug. The elevated level of blood glucose, glycosylated haemoglobin, TBARS observed in diabetic rats were significantly altered compared to control but it not come to normal level after treatment with MKL for 15 days in diabetic rats. From the resul...

damage. Administration of DSS to experimental rats in a drinking water causes human UC-like patho... more damage. Administration of DSS to experimental rats in a drinking water causes human UC-like pathologies in which it causes toxicity to the epithelial cells of the colon which further cause disturbances in the mucosal barrier function.[5] There are several types of medications which are used for the treatment of colitis by controlling inf lammation or reduce symptoms of colitis. Herbal medicine or traditional medicines includes an extensive range of practices, as well as, therapies. Herbal drugs are safer than synthetic drug, and therefore, preferred widely to treat various ailments. Some potential benefits of herbal drugs that their acceptance rate by the patient is high, they have good efficacy, relatively safe, and have low cost.[6] In present study hydroalcoholic extract of H. isora dried fruit was used. H. isora L. (Malvaceae), is one of Prajakta Murudkar et al. Int. J. Pharm. Sci. Drug Res. July-August, 2020, Vol 12, Issue 4, 325-330 326 Q5 the medicinally important tree specie...

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This study was conducted to ascertain the attitudes of undergraduate pharmacy students to animal ... more This study was conducted to ascertain the attitudes of undergraduate pharmacy students to animal experimentation after they had completed experiments on frog abdominal rectus muscle, goat intestine, studied the effect of various drug like analgesic, anti-inflammatory, diuretics etc on mice and rats and on computer programme in academic year 2008-09. Barely sixty five percent of the students agreed with to use alternative of life animals like mice and rats for experimentation purpose in education, an overwhelming majority (seventy four percent) felt that the animal experiments involved needless pain and suffering to the animals. A large majority of students felt the need to reduce the number of animals by demonstrating the practical, to explore alternatives to the animal experiments, and to restrict animal experiments for research on life threatening diseases. More than half of the students were unaware of the government regulations on animal experiments. Students considered, underst...

Ovarian hormone decline after menopause is linked to many pathophysiological reactions. Dyslipida... more Ovarian hormone decline after menopause is linked to many pathophysiological reactions. Dyslipidaemia is often seen in postmenopausal women and is characterized by an overall shift toward a more atherogenic lipid profile (Juliana et al., 2011; Rachon et al., 2008). Its incidence increases after menopause due to decreased oestrogen level, since oestrogens are involved in cholesterol metabolism by lowering LDL and increasing HDL concentrations in plasma (Juliana et al., 2011; El-Swefy et al., 2002). It is well known that oestrogens, acting as free radical scavengers, break the free radical chain formation produced from membrane oxidation processes and hence inhibit lipid and protein oxidation (Juliana et al., 2011; Akçay et al., 2000). Estrogens have antioxidant properties and can inhibit lipid peroxidation in vitro (Saeedeh et al., 2013; Arteaga et al., 2003). After menopause, the incidence of cardiovascular disease increases (Saeedeh et al., 2013; Castelao et al., 2008). As antioxid...