Nripendra Nath Biswas | Khulna University (original) (raw)
Papers by Nripendra Nath Biswas
International Journal of Pharmaceutical Sciences Review and Research
This study aims to explore analgesic, antidiarrheal, neuropharmacological, and antibacterial acti... more This study aims to explore analgesic, antidiarrheal, neuropharmacological, and antibacterial activities of petroleum ether and water fractions of Justicia aurea (J. aurea). The results of analgesic activity showed that both nonpolar (petroleum ether) and polar (water) fractions of J. aurea extract at 250 and 500 mg/kg significantly suppressed the writhing reflex dose-dependently. Here, the potentiality of petroleum ether fraction was higher than that of water fraction. In the castor oil-induced diarrheal mice, both fractions of J. aurea extract, at 250 and 500 mg/kg, significantly delayed the onset of diarrhea and lessened the total number of feces in a dose-dependent manner. Of the two fractions, the nonpolar fraction was found to exhibit a better effect. Both fractions appreciably reduced the number of squares crossed by the mice at both 250 and 500 mg/kg. At 500 mg/kg, petroleum ether fraction reflected a slightly better effect than that of water fraction. In disc diffusion antib...
Khulna University Studies
Moringa oleifera (Family- Moringaceae) is a well-known vegetable for its health benefits for thou... more Moringa oleifera (Family- Moringaceae) is a well-known vegetable for its health benefits for thousands of years having diverse nutritional bioactive constituents. Phytochemical analysis of ethanolic extract of this plant indicated the presence of carbohydrate, reducing sugar, combined reducing sugar, glycosides, tannins (absent in seeds), alkaloids, flavonoids, saponins and steroids. In diuretic activity test, the extract showed a significant (p<0.001) effect at the dose of 200 & 400 mg/kg body weight in production of total urine volume in comparison with standard drug furosemide (5 mg/kg). Upon electrolyte analysis of excreted urine, the extract was found to increase Na+, K+, Clexcretion and may act as loop diuretics. The pods and seeds extract showed significant (p<0.01) laxative effects in mice in terms of stool production with soft consistency at the dose of 200 & 400 mg/kg in comparison with standard drug bisacodyl (10 mg/kg). The most edible part of this plant is pods wh...
The present study was undertaken to investigate the toxicological effects of Rasnairandadi Kvatha... more The present study was undertaken to investigate the toxicological effects of Rasnairandadi Kvatha Curna (RAS), a classical ayurvedic preparations used as an analgesic drug.Various biochemical parameters such as total protein, serum albumin, bilirubin, creatinine, and liver enzymes i.e. serum glutamic pyruvic transaminase (sGPT), serum glutamic oxaloacetic transaminase (sGOT) and alkaline phosphatase (ALP) of experimental rats were analysed after chronic administration of RAS. The total protein content showed no significant difference between the control and the RAS treated both male and female rats. Albumin content significantly increased (p<0.001) in both RAS treated male and female compared to control. There was a significant increase of sGPT (p<0.001) and sGOT (p<0.05) activities found in the plasma of RAS treated male rats compared to control. Also, ALP levels significantly (p<0.001) increased in the plasma of RAS treated male rats. On the contrary, RAS treated femal...
Journal of Medicinal Plants Studies, 2021
Stenochlaena palustris is a widely found fern in mangrove forests and other areas of different co... more Stenochlaena palustris is a widely found fern in mangrove forests and other areas of different countries over the world. This plant has many uses in traditional medicine of many countries. This study was conducted on its leaves extract to investigate its different phytochemical and pharmacological properties based on its local medicinal uses. Phytochemical screening indicated the presence of different secondary metabolites in this plant. On silica coated TLC plates, the extract exhibited the presence of different antioxidative compounds. Total phenolic, flavonoid and tannin contents were determined (97 mg GAE/g, 90 mg QE/g and 23 mg GAE/g, respectively). This extract also scavenged DPPH, hydrogen peroxide and superoxide radicals (SC50 = 80, 158 and 236 μg/ml, respectively). It also showed ferric ion reducing capability (RC50 =166 μg/ml). This plant showed significant antihyperglycemic, peripheral analgesic (acetic acid induced), anti-inflammatory (formaldehyde induced) and laxative activities in mice. This plant also showed little cytotoxic activity in brine shrimp lethality bioassay. These results are also compared with respective standard drugs and our findings justified its traditional usage.
Dhaka University Journal of Pharmaceutical Sciences, 2007
Dhaka Univ. J. Pharm. Sci. Vol.4(1) 2005 The full text is of this article is available at the Dha... more Dhaka Univ. J. Pharm. Sci. Vol.4(1) 2005 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website
American Journal of Biochemistry and Biotechnology, 2013
Araucaria cookii, Bauhinia blakeana and Brassaia actinophylla are ornamental plants. The presence... more Araucaria cookii, Bauhinia blakeana and Brassaia actinophylla are ornamental plants. The presence of various phytochemicals and pharmacologically important compounds in these plants can be exploited for their medicinal use. But there are no reports on the phytochemical and pharmacological evaluation of these plants and this study aims at investigating these. The plant extracts were prepared in different solvents like methanol, ethanol, ethyl acetate, acetone, hexane, water and chloroform. Qualitative analysis of phytochemicals were assessed. The anti-microbial, anti-oxidant and enzyme inhibitory activity was determined for all three plant extracts. Anti-bacterial activity against three gram negative bacteria, E. coli, Pseudomonas and Klebsiella was done and Araucaria cooki showed highest antibacterial activity among the three plants. Maximum antioxidant activity was seen in methanol extract of Brassaia actinophyla with 81% inhibition. The order of the antioxidant activity of the three plants are in the order B.actinophylla>A cookie>B.blakeana. The results of phytochemical analysis suggest that phytosteroids are present in all the three plants. Maximum inhibition against the tested enzymes was exhibited by hexane and chloroform extracts of A.cookii. Hemolytic activity was done and the hexane extract showed maximum haemolysis where as aqueous extracts showed minimum activity. From the results it is clear that the three plant extracts has pharmacological applications. This is the first report of antimicrobial, antioxidant and enzyme inhibitory activities of these three plant extracts. Further studies are needed to exploit the actual mechanism and active compounds of these plants.
International Journal of Advances in Scientific Research, 2015
Purpose: To investigate potential analgesic and antioxidant properties in the ethanolic root extr... more Purpose: To investigate potential analgesic and antioxidant properties in the ethanolic root extract of Clerodendrum viscosum Vent. Methods: Phytochemical analysis of the ethanolic root extract was evaluated using standard procedure. Analgesic activity test was carried out by acetic acid induced writhing in mice model. The antioxidant property of the plant extract was assessed by DPPH (1, 1-diphenyl-2-picryl hydrazyl) free radical scavenging activity. Results: Phytochemical analysis displayed that the plant extract contains carbohydrates, glycosides, tannins, saponins, flavonoids and steroids types of compounds. The extract exhibited dose-dependent and statistically significant (P<0.01) writhing inhibition in acetic acid induced mice. The extract produced about 38.59 % & 59.07% writhing inhibition at the doses of 250 mg/kg & 500 mg/kg respectively, which was comparable to the standard drug diclofenac sodium (writhing inhibition was about 77.78% at the dose of 25 mg/kg). In DPPH s...
Journal of Medicinal Plants Studies, 2021
Background: Ceriops decandra (Griff.) Ding Hou is used conventionally for managing a number of th... more Background: Ceriops decandra (Griff.) Ding Hou is used conventionally for managing a number of therapeutic conditions including diabetes, haemorrhage, pain, diarrhea, angina, and dysentery etc. Furthermore, Ceriops tagal Linn is used to treat hemorrhage, acariasis, wound infection, malaria, malignant diabetes and ulcers etc in folk medicine. The present study was designed to evaluate the comparative pharmacological activities of leaves of these two medicinal plants of the Sundarbans. Methods: Antioxidant activity was assessed by total phenolic, total flavonoids, total tannin content, and DPPH free radical scavenging assays. Acetic acid-induced test for analgesic, oral glucose tolerance test for antihyperglycemic, open field test for neurobehavioral, prothrombin time test for anticoagulant, anthelmintic potential on Paramphistomum cervi, and in-vivo cytotoxicity test on brine shrimp nauplii were used for appraising pharmacological activities of both extracts. Results: C. decandra extract revealed greater radical scavenging activity than that of C. tagal. C. decandra extract significantly (P < 0.05) reduced writhing inhibition of 54.55% and 66.37% at 500mg/kg and 250 mg/kg, respectively. In oral glucose tolerance test, C. decandra significantly lowered the blood glucose level for pretreated mice with glucose by 25.76%, 2272%, and 31.88%, 38.71% respectively, at 60 min and 120min at the 250 mg/kg, and 500 mg/kg dose level. But, the reduction of blood glucose level by C. tagal extract was less than that of C. decandra. Furthermore, C. decandra produced a sedative effect at both doses starting from 30 min to 120 min of experimental period. The crude extract of C. decandra and C. tagal delayed the prothrombin time in a dose dependently. The C. decandra and C. tagal produced dose-dependent paralysis and death time. The LC50 values obtained from brine shrimp lethality bioassay were 94.69, 114.29 µg/mL for C. decandra and C. tagal, respectively. Conclusion: C. decandra revealed prominent pharmacological activities than that of C. tagal. Also, the present study confirmed the scientific evidence of both plants.
Advances in Traditional Medicine
Evidence-Based Complementary and Alternative Medicine
Background. Avicennia officinalis is a medicinal plant that has traditionally been used as a diur... more Background. Avicennia officinalis is a medicinal plant that has traditionally been used as a diuretic, anti-infective, and antiasthmatic. Our investigation was designed to explore the diuretic and laxative potentials of different fractions of this plant’s bark extract as well as the identification of possible drug candidates for the activity. Methods. Collected bark was extracted in ethanol and fractionated in different polar and nonpolar solvents, i.e., water, chloroform, ethyl acetate, and n-hexane. Phytoconstituents were identified following the published protocols and gas chromatography-mass spectrometry (GC-MS). In the diuretic test, Na+ and K+ ions were measured using a flame photometer whereas the Cl− ion content was measured by titrimetric method against AgNO3. In the laxative test, feces amount and consistency were also measured. Molecular docking analysis was conducted using the “Vina Wizard” program in PyRx-Python Prescription 0.8. Results. Phytochemical analysis indicate...
Background Sonneratia caseolaris (L.) Engl. ( S. caseolaris ) belonging to the Sonneratiaceae fam... more Background Sonneratia caseolaris (L.) Engl. ( S. caseolaris ) belonging to the Sonneratiaceae family is commonly known as Ora. It is traditionally used as an astringent, antiseptic, to treat sprains, swellings, cough and in arresting hemorrhage. The ethanolic extract of S. caseolaris (L.) Engl. fruits was investigated in the present study for its toxicity as well as anti-allergic and anti-hyperglycemic potentials. Methods Major phenolic compounds were identified and quantified by HPLC. Behavioral change, body weight, mortality and different blood parameters were measured to assess the toxicological effect of the extract. Anti-allergic activity was evaluated using TDI-induced allergic model mice. Oral glucose tolerance test (OGTT) and STZ-induced diabetic mice were used to evaluate the anti-hyperglycemic activity. Results Crude extract contained ellagic acid, vanillic acid and myrecitin (27.41, 3.06 and 7.93 mg per 100 g dry extract respectively). No major toxicity was observed in bo...
Journal of Medicinal Plants Studies, 2021
Different fractionated extracts (in ethanol, n-hexane, chloroform, ethyl acetate and water) of tw... more Different fractionated extracts (in ethanol, n-hexane, chloroform, ethyl acetate and water) of two mangrove plants Kandelia candel and Brownlowia tersa have been investigated here for diuretic and laxative bioactivity. In diuretic test, frusemide (5 mg/kbw) was used as standard and the parameters like volume of urine up to 6 th hour, pH, density, conductivity and Na + , K + , Clcontents of collected urine were measured. Ethyl acetate fraction of Kandelia candel showed significant diuretic activity (p<0.001) at 200 mg/kbw and 400 mg/kbw dose compared with control. For aerial parts of Brownlowia tersa, chloroform fraction demonstrated better diuretic activity. Both the active fractions were found to work as loop diuretics. In laxative activity test, bisacodyl (10 mg/kbw) was used as standard and a significant percent increment of fecal output (p<0.0001) was observed for ethyl acetate fraction of Kandelia candel at both the doses. For Brownlowia tersa, the ethyl acetate and water fractions demonstrated significant laxative effect. Phytochemical analysis for both the plants extracts exhibited the presence of carbohydrate, reducing sugar, steroids, saponins, glycosides, tannins, alkaloids, terpenoids and flavonoids etc. Further studies may be carried to isolate and characterize the active compound(s) responsible for the claimed activity.
Journal of Chinese Integrative Medicine, 2011
ABSTRACT To evaluate the antinociceptive, anti-inflammatory and antidiarrheal activities of the e... more ABSTRACT To evaluate the antinociceptive, anti-inflammatory and antidiarrheal activities of the ethanolic calyx extract of Hibiscus sabdariffa Linn. in mice. In the present study, the dried calyxes of H. sabdariffa were subjected to extraction with 95% ethanol and the extract was used to investigate the possible activities. Antinociceptive activity of the extract was evaluated by using the acetic acid-induced writhing test. The anti-inflammatory effect of the extract was tested by using the xylene-induced ear edema model mice. Castor oil-induced diarrheal model mice were used to evaluate the antidiarrheal activity of the extract. In acetic acid-induced writhing test, the extract produced inhibited writhing in mice significantly compared with the blank control (P<0.01). The extract showed significant inhibition of ear edema formation in xylene-induced ear edema model mice in a dose-related manner compared with the blank control (P<0.01). The extract demonstrated a significant antidiarrheal activity against castor oil-induced diarrheal in mice in which it decreased the frequency of defecation and increased the mean latent period at the doses of 250 and 500 mg/kg body weight (P<0.01). The above mentioned findings indicate that the calyx extract of H. sabdariffa possesses significant antinociceptive, anti-inflammatory and antidiarrheal activities that support its uses in traditional medicine.
Organic & biomolecular chemistry, Jan 21, 2015
Gram-negative bacteria such as Pseudomonas aeruginosa use N-acylated l-homoserine lactones (AHLs)... more Gram-negative bacteria such as Pseudomonas aeruginosa use N-acylated l-homoserine lactones (AHLs) as autoinducers (AIs) for quorum sensing (QS), a major regulatory and cell-to-cell communication system for social adaptation, virulence factor production, biofilm formation and antibiotic resistance. Some bacteria use indole moieties for intercellular signaling and as regulators of various bacterial phenotypes important for evading the innate host immune response and antimicrobial resistance. A range of natural and synthetic indole derivatives have been found to act as inhibitors of QS-dependent bacterial phenotypes, complementing the bactericidal ability of traditional antibiotics. In this work, various indole-based AHL mimics were designed and synthesized via the 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC·HCl) and N,N'-dicyclohexylcarbodiimide (DCC) mediated coupling reactions of a variety of substituted or unsubstituted aminoindoles with different alkanoic ...
Dhaka University Journal of Pharmaceutical Sciences, 2007
Dhaka Univ. J. Pharm. Sci. Vol.4(1) 2005 The full text is of this article is available at the Dha... more Dhaka Univ. J. Pharm. Sci. Vol.4(1) 2005 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website
Bangladesh Pharmaceutical Journal
Traditional medicines including Ayurveda, Unani have been used as therapeutics in the Indian subc... more Traditional medicines including Ayurveda, Unani have been used as therapeutics in the Indian subcontinent since 5000 BC. Because of the abundance of secondary metabolites, medicinal plants are becoming priceless natural resources for better drug development. Till date almost no ethnopharmacological evidences were available on Colocasia genus except Colocasia esculenta. As a part of our continuing assessment of evidence based use of traditional medicine, we have identified bioactive polyphenols in the ethanolic leaves extract of Colocasia affinis Schott using HPLC-DAD method and evaluated its anti-allergy, anti-inflammatory and anti-diabetic potentials for the first time. Chromatographic investigation showed the presence of p-coumaric acid, trans-ferulic acid, rosmarinic acid, myricetin and kaempferol in the leaves extract. Pharmacological profile exploration demonstrated its anti-inflammatory activity at a dose of 250- and 500-mg/kg in xylene-induced ear edema in mice. The extract s...
Clinical Phytoscience
Background: The root-bark of the medicinally important plant Zanthoxylum budrunga (ZBRB) brooks a... more Background: The root-bark of the medicinally important plant Zanthoxylum budrunga (ZBRB) brooks a variety of uses in ethnobotanical and ethnomedicinal practice in Bangladesh and thus demands biological investigation to reveal its therapeutic potentiality. So, the present study was perpetrated to explore antioxidant, analgesic, antidiarrhoeal, and a cytotoxic activity of ethanolic root-bark extract of Zanthoxylum budrunga and was also to quantify the major bioactive polyphenolic constituents by HPLC analysis. Methods: Total phenolic content was measured spectrophotometrically by using Folin Chiocalteu's reagent while in vitro antioxidant activity was determined by means of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and reducing power assay. HPLC analysis was performed to identify and quantify the major bioactive polyphenolic constituents present in the extract. Acetic acid induced writhing test and hot-plate test were conducted to evaluate the analgesic activity of the crude extract. On the other hand, in vivo antidiarrheal potentiality was investigated by using experimentally castor oil induced diarrhoea in mice and brine shrimp lethality bio-assay was implemented to check the cytotoxic potentiality of the crude extract. Results: Zanthoxylum budrunga showed DPPH scavenging activity (IC 50 54.27 μg/mL), while the total phenolic content was 647.91 mg GAE/100 g of extract. ZBRB also showed concentration dependent ferric reducing power activity. At the doses of 250 and 500 mg/kg, ZBRB exhibited statistically significant (P < 0.001) inhibition of writhing in test mice (64.58 and 77.78%, respectively). In hot-plate test, ZBRB, at the above dose levels, significantly (P < 0.001) prolonged pain threshold with response time of 5.80 and 6.81 s, respectively. In castor oil induced diarrhoeal episode in mice, ZBRB exhibited 66.56% and 83.39% inhibition of defecation at the doses of 250 and 500 mg/kg, respectively (P < 0.001). The LC 50 (Median lethal concentration) value of ZBRB in brine shrimp lethality bioassay was found to be 21. 84 μg/mL. In the HPLC analysis, (+)-catechin, caffeic acid and quercetin were detected with the concentration of 17. 94 mg/100, 3.72 mg/100 and 11.95 mg/100 g of ethanolic extract of ZBRB, respectively. Conclusion: The results rationalize the uses of the plant in traditional medicine for diarrhoeal as well as pain management. Catechin, caffeic acid, and other phenolics constituents might have some function in the observed activity.
Clinical Phytoscience
Background: Acanthus ilicifolius L. is admired for its traditional usage in the folk medicine for... more Background: Acanthus ilicifolius L. is admired for its traditional usage in the folk medicine for the treatment of numerous diseases including allergy and helminthiasis in various parts of the planet. The ethanolic extract of the aerial parts of this shrub (EEAI) was investigated in the present study for its major phytochemical groups, antiallergic activity, anthelmintic activity, cytotoxicity and for acute toxicity. Methods: Antiallergic activity was carried out using Toluene 2, 4-diisocyanate (TDI)-induced allergic mice model by assessing various symptoms of allergic rhinitis like sneezing, scratching, swelling and watery rhinorrhea as well as counting the total and differential leukocytes profile of blood. The paralysis and death time of parasites, Haemonchus contortus (Nematoda) and Paramphistomum cervi (Trematoda) were used for anthelmintic activity test. Mortality of mice was counted to evaluate the acute toxicity whereas the mortality of brine shrimp was taken into account to assess cytotoxic potential of the extract. Results: Phytochemical screening of the extract demonstrated the presence of alkaloids, phenolic compounds, tannins, flavonoids, glycosides, saponins, steroids and triterpenoids. Oral pretreatment of the extract significantly ameliorated the TDI-induced allergic symptoms like sneezing (p < 0.05), scratching (p < 0.05), swelling and watery rhinorrhea in experimental mice. The extract also reduced the differential count of leukocytes in the blood which was increased due to induction of allergic conditions through TDI sensitization. In anthelmintic activity test the extract revealed a dose dependent decrease in the relative index of paralysis and death for both H. contortus and P. cervi parasites and thus indicated the extract to be parasiticidal at higher concentrations. In brine shrimp lethality bioassay of toxicity assessment, the LC 50 of the standard drug vincristine sulfate was 0.43 μg/mL whereas the extract showed the LC 50 as 44.57 μg/mL indicating a promising cytotoxicity of the extract. In acute toxicity study the highest dose 3 g/kg failed to show any mortality in Swiss albino mice and thus confirmed the safety of the extract for in vivo administration. Conclusions: The present study corroborated the traditional uses of the aerial parts of Acanthus ilicifolius L. in allergic diseases and in helminthiasis.
Australian Journal of Chemistry
Gram-negative bacteria such as Pseudomonas aeruginosa use furanosyl diesters as autoinducers for ... more Gram-negative bacteria such as Pseudomonas aeruginosa use furanosyl diesters as autoinducers for quorum sensing (QS), a major regulatory and cell-to-cell communication system for social adaptation, virulence factor production, biofilm formation, and antibiotic resistance. A range of natural and synthetic brominated furanones, i.e. fimbrolide derivatives, have been found to act as inhibitors of QS-dependent bacterial phenotypes, complementing the bactericidal ability of traditional antibiotics. In this work, several novel acetylene analogues of fimbrolides were synthesised in moderate to high yields via Sonogashira coupling reactions of brominated furanones 4-bromo-5-(bromomethylene)furan-2(5H)-one 4 and 5-(dibromomethylene)-3-ethylfuran-2(5H)-one 5. The Sonogashira reaction of acetylenes on 4-bromo-5-(bromomethylene)furan-2(5H)-one 4 was favoured at the C5 methylene bromide over the C4 bromide substituent. On biological testing, the most potent compounds 13 and 14 showed 82 and 98 %...
International Journal of Pharmaceutical Sciences Review and Research
This study aims to explore analgesic, antidiarrheal, neuropharmacological, and antibacterial acti... more This study aims to explore analgesic, antidiarrheal, neuropharmacological, and antibacterial activities of petroleum ether and water fractions of Justicia aurea (J. aurea). The results of analgesic activity showed that both nonpolar (petroleum ether) and polar (water) fractions of J. aurea extract at 250 and 500 mg/kg significantly suppressed the writhing reflex dose-dependently. Here, the potentiality of petroleum ether fraction was higher than that of water fraction. In the castor oil-induced diarrheal mice, both fractions of J. aurea extract, at 250 and 500 mg/kg, significantly delayed the onset of diarrhea and lessened the total number of feces in a dose-dependent manner. Of the two fractions, the nonpolar fraction was found to exhibit a better effect. Both fractions appreciably reduced the number of squares crossed by the mice at both 250 and 500 mg/kg. At 500 mg/kg, petroleum ether fraction reflected a slightly better effect than that of water fraction. In disc diffusion antib...
Khulna University Studies
Moringa oleifera (Family- Moringaceae) is a well-known vegetable for its health benefits for thou... more Moringa oleifera (Family- Moringaceae) is a well-known vegetable for its health benefits for thousands of years having diverse nutritional bioactive constituents. Phytochemical analysis of ethanolic extract of this plant indicated the presence of carbohydrate, reducing sugar, combined reducing sugar, glycosides, tannins (absent in seeds), alkaloids, flavonoids, saponins and steroids. In diuretic activity test, the extract showed a significant (p<0.001) effect at the dose of 200 & 400 mg/kg body weight in production of total urine volume in comparison with standard drug furosemide (5 mg/kg). Upon electrolyte analysis of excreted urine, the extract was found to increase Na+, K+, Clexcretion and may act as loop diuretics. The pods and seeds extract showed significant (p<0.01) laxative effects in mice in terms of stool production with soft consistency at the dose of 200 & 400 mg/kg in comparison with standard drug bisacodyl (10 mg/kg). The most edible part of this plant is pods wh...
The present study was undertaken to investigate the toxicological effects of Rasnairandadi Kvatha... more The present study was undertaken to investigate the toxicological effects of Rasnairandadi Kvatha Curna (RAS), a classical ayurvedic preparations used as an analgesic drug.Various biochemical parameters such as total protein, serum albumin, bilirubin, creatinine, and liver enzymes i.e. serum glutamic pyruvic transaminase (sGPT), serum glutamic oxaloacetic transaminase (sGOT) and alkaline phosphatase (ALP) of experimental rats were analysed after chronic administration of RAS. The total protein content showed no significant difference between the control and the RAS treated both male and female rats. Albumin content significantly increased (p<0.001) in both RAS treated male and female compared to control. There was a significant increase of sGPT (p<0.001) and sGOT (p<0.05) activities found in the plasma of RAS treated male rats compared to control. Also, ALP levels significantly (p<0.001) increased in the plasma of RAS treated male rats. On the contrary, RAS treated femal...
Journal of Medicinal Plants Studies, 2021
Stenochlaena palustris is a widely found fern in mangrove forests and other areas of different co... more Stenochlaena palustris is a widely found fern in mangrove forests and other areas of different countries over the world. This plant has many uses in traditional medicine of many countries. This study was conducted on its leaves extract to investigate its different phytochemical and pharmacological properties based on its local medicinal uses. Phytochemical screening indicated the presence of different secondary metabolites in this plant. On silica coated TLC plates, the extract exhibited the presence of different antioxidative compounds. Total phenolic, flavonoid and tannin contents were determined (97 mg GAE/g, 90 mg QE/g and 23 mg GAE/g, respectively). This extract also scavenged DPPH, hydrogen peroxide and superoxide radicals (SC50 = 80, 158 and 236 μg/ml, respectively). It also showed ferric ion reducing capability (RC50 =166 μg/ml). This plant showed significant antihyperglycemic, peripheral analgesic (acetic acid induced), anti-inflammatory (formaldehyde induced) and laxative activities in mice. This plant also showed little cytotoxic activity in brine shrimp lethality bioassay. These results are also compared with respective standard drugs and our findings justified its traditional usage.
Dhaka University Journal of Pharmaceutical Sciences, 2007
Dhaka Univ. J. Pharm. Sci. Vol.4(1) 2005 The full text is of this article is available at the Dha... more Dhaka Univ. J. Pharm. Sci. Vol.4(1) 2005 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website
American Journal of Biochemistry and Biotechnology, 2013
Araucaria cookii, Bauhinia blakeana and Brassaia actinophylla are ornamental plants. The presence... more Araucaria cookii, Bauhinia blakeana and Brassaia actinophylla are ornamental plants. The presence of various phytochemicals and pharmacologically important compounds in these plants can be exploited for their medicinal use. But there are no reports on the phytochemical and pharmacological evaluation of these plants and this study aims at investigating these. The plant extracts were prepared in different solvents like methanol, ethanol, ethyl acetate, acetone, hexane, water and chloroform. Qualitative analysis of phytochemicals were assessed. The anti-microbial, anti-oxidant and enzyme inhibitory activity was determined for all three plant extracts. Anti-bacterial activity against three gram negative bacteria, E. coli, Pseudomonas and Klebsiella was done and Araucaria cooki showed highest antibacterial activity among the three plants. Maximum antioxidant activity was seen in methanol extract of Brassaia actinophyla with 81% inhibition. The order of the antioxidant activity of the three plants are in the order B.actinophylla>A cookie>B.blakeana. The results of phytochemical analysis suggest that phytosteroids are present in all the three plants. Maximum inhibition against the tested enzymes was exhibited by hexane and chloroform extracts of A.cookii. Hemolytic activity was done and the hexane extract showed maximum haemolysis where as aqueous extracts showed minimum activity. From the results it is clear that the three plant extracts has pharmacological applications. This is the first report of antimicrobial, antioxidant and enzyme inhibitory activities of these three plant extracts. Further studies are needed to exploit the actual mechanism and active compounds of these plants.
International Journal of Advances in Scientific Research, 2015
Purpose: To investigate potential analgesic and antioxidant properties in the ethanolic root extr... more Purpose: To investigate potential analgesic and antioxidant properties in the ethanolic root extract of Clerodendrum viscosum Vent. Methods: Phytochemical analysis of the ethanolic root extract was evaluated using standard procedure. Analgesic activity test was carried out by acetic acid induced writhing in mice model. The antioxidant property of the plant extract was assessed by DPPH (1, 1-diphenyl-2-picryl hydrazyl) free radical scavenging activity. Results: Phytochemical analysis displayed that the plant extract contains carbohydrates, glycosides, tannins, saponins, flavonoids and steroids types of compounds. The extract exhibited dose-dependent and statistically significant (P<0.01) writhing inhibition in acetic acid induced mice. The extract produced about 38.59 % & 59.07% writhing inhibition at the doses of 250 mg/kg & 500 mg/kg respectively, which was comparable to the standard drug diclofenac sodium (writhing inhibition was about 77.78% at the dose of 25 mg/kg). In DPPH s...
Journal of Medicinal Plants Studies, 2021
Background: Ceriops decandra (Griff.) Ding Hou is used conventionally for managing a number of th... more Background: Ceriops decandra (Griff.) Ding Hou is used conventionally for managing a number of therapeutic conditions including diabetes, haemorrhage, pain, diarrhea, angina, and dysentery etc. Furthermore, Ceriops tagal Linn is used to treat hemorrhage, acariasis, wound infection, malaria, malignant diabetes and ulcers etc in folk medicine. The present study was designed to evaluate the comparative pharmacological activities of leaves of these two medicinal plants of the Sundarbans. Methods: Antioxidant activity was assessed by total phenolic, total flavonoids, total tannin content, and DPPH free radical scavenging assays. Acetic acid-induced test for analgesic, oral glucose tolerance test for antihyperglycemic, open field test for neurobehavioral, prothrombin time test for anticoagulant, anthelmintic potential on Paramphistomum cervi, and in-vivo cytotoxicity test on brine shrimp nauplii were used for appraising pharmacological activities of both extracts. Results: C. decandra extract revealed greater radical scavenging activity than that of C. tagal. C. decandra extract significantly (P < 0.05) reduced writhing inhibition of 54.55% and 66.37% at 500mg/kg and 250 mg/kg, respectively. In oral glucose tolerance test, C. decandra significantly lowered the blood glucose level for pretreated mice with glucose by 25.76%, 2272%, and 31.88%, 38.71% respectively, at 60 min and 120min at the 250 mg/kg, and 500 mg/kg dose level. But, the reduction of blood glucose level by C. tagal extract was less than that of C. decandra. Furthermore, C. decandra produced a sedative effect at both doses starting from 30 min to 120 min of experimental period. The crude extract of C. decandra and C. tagal delayed the prothrombin time in a dose dependently. The C. decandra and C. tagal produced dose-dependent paralysis and death time. The LC50 values obtained from brine shrimp lethality bioassay were 94.69, 114.29 µg/mL for C. decandra and C. tagal, respectively. Conclusion: C. decandra revealed prominent pharmacological activities than that of C. tagal. Also, the present study confirmed the scientific evidence of both plants.
Advances in Traditional Medicine
Evidence-Based Complementary and Alternative Medicine
Background. Avicennia officinalis is a medicinal plant that has traditionally been used as a diur... more Background. Avicennia officinalis is a medicinal plant that has traditionally been used as a diuretic, anti-infective, and antiasthmatic. Our investigation was designed to explore the diuretic and laxative potentials of different fractions of this plant’s bark extract as well as the identification of possible drug candidates for the activity. Methods. Collected bark was extracted in ethanol and fractionated in different polar and nonpolar solvents, i.e., water, chloroform, ethyl acetate, and n-hexane. Phytoconstituents were identified following the published protocols and gas chromatography-mass spectrometry (GC-MS). In the diuretic test, Na+ and K+ ions were measured using a flame photometer whereas the Cl− ion content was measured by titrimetric method against AgNO3. In the laxative test, feces amount and consistency were also measured. Molecular docking analysis was conducted using the “Vina Wizard” program in PyRx-Python Prescription 0.8. Results. Phytochemical analysis indicate...
Background Sonneratia caseolaris (L.) Engl. ( S. caseolaris ) belonging to the Sonneratiaceae fam... more Background Sonneratia caseolaris (L.) Engl. ( S. caseolaris ) belonging to the Sonneratiaceae family is commonly known as Ora. It is traditionally used as an astringent, antiseptic, to treat sprains, swellings, cough and in arresting hemorrhage. The ethanolic extract of S. caseolaris (L.) Engl. fruits was investigated in the present study for its toxicity as well as anti-allergic and anti-hyperglycemic potentials. Methods Major phenolic compounds were identified and quantified by HPLC. Behavioral change, body weight, mortality and different blood parameters were measured to assess the toxicological effect of the extract. Anti-allergic activity was evaluated using TDI-induced allergic model mice. Oral glucose tolerance test (OGTT) and STZ-induced diabetic mice were used to evaluate the anti-hyperglycemic activity. Results Crude extract contained ellagic acid, vanillic acid and myrecitin (27.41, 3.06 and 7.93 mg per 100 g dry extract respectively). No major toxicity was observed in bo...
Journal of Medicinal Plants Studies, 2021
Different fractionated extracts (in ethanol, n-hexane, chloroform, ethyl acetate and water) of tw... more Different fractionated extracts (in ethanol, n-hexane, chloroform, ethyl acetate and water) of two mangrove plants Kandelia candel and Brownlowia tersa have been investigated here for diuretic and laxative bioactivity. In diuretic test, frusemide (5 mg/kbw) was used as standard and the parameters like volume of urine up to 6 th hour, pH, density, conductivity and Na + , K + , Clcontents of collected urine were measured. Ethyl acetate fraction of Kandelia candel showed significant diuretic activity (p<0.001) at 200 mg/kbw and 400 mg/kbw dose compared with control. For aerial parts of Brownlowia tersa, chloroform fraction demonstrated better diuretic activity. Both the active fractions were found to work as loop diuretics. In laxative activity test, bisacodyl (10 mg/kbw) was used as standard and a significant percent increment of fecal output (p<0.0001) was observed for ethyl acetate fraction of Kandelia candel at both the doses. For Brownlowia tersa, the ethyl acetate and water fractions demonstrated significant laxative effect. Phytochemical analysis for both the plants extracts exhibited the presence of carbohydrate, reducing sugar, steroids, saponins, glycosides, tannins, alkaloids, terpenoids and flavonoids etc. Further studies may be carried to isolate and characterize the active compound(s) responsible for the claimed activity.
Journal of Chinese Integrative Medicine, 2011
ABSTRACT To evaluate the antinociceptive, anti-inflammatory and antidiarrheal activities of the e... more ABSTRACT To evaluate the antinociceptive, anti-inflammatory and antidiarrheal activities of the ethanolic calyx extract of Hibiscus sabdariffa Linn. in mice. In the present study, the dried calyxes of H. sabdariffa were subjected to extraction with 95% ethanol and the extract was used to investigate the possible activities. Antinociceptive activity of the extract was evaluated by using the acetic acid-induced writhing test. The anti-inflammatory effect of the extract was tested by using the xylene-induced ear edema model mice. Castor oil-induced diarrheal model mice were used to evaluate the antidiarrheal activity of the extract. In acetic acid-induced writhing test, the extract produced inhibited writhing in mice significantly compared with the blank control (P<0.01). The extract showed significant inhibition of ear edema formation in xylene-induced ear edema model mice in a dose-related manner compared with the blank control (P<0.01). The extract demonstrated a significant antidiarrheal activity against castor oil-induced diarrheal in mice in which it decreased the frequency of defecation and increased the mean latent period at the doses of 250 and 500 mg/kg body weight (P<0.01). The above mentioned findings indicate that the calyx extract of H. sabdariffa possesses significant antinociceptive, anti-inflammatory and antidiarrheal activities that support its uses in traditional medicine.
Organic & biomolecular chemistry, Jan 21, 2015
Gram-negative bacteria such as Pseudomonas aeruginosa use N-acylated l-homoserine lactones (AHLs)... more Gram-negative bacteria such as Pseudomonas aeruginosa use N-acylated l-homoserine lactones (AHLs) as autoinducers (AIs) for quorum sensing (QS), a major regulatory and cell-to-cell communication system for social adaptation, virulence factor production, biofilm formation and antibiotic resistance. Some bacteria use indole moieties for intercellular signaling and as regulators of various bacterial phenotypes important for evading the innate host immune response and antimicrobial resistance. A range of natural and synthetic indole derivatives have been found to act as inhibitors of QS-dependent bacterial phenotypes, complementing the bactericidal ability of traditional antibiotics. In this work, various indole-based AHL mimics were designed and synthesized via the 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC·HCl) and N,N'-dicyclohexylcarbodiimide (DCC) mediated coupling reactions of a variety of substituted or unsubstituted aminoindoles with different alkanoic ...
Dhaka University Journal of Pharmaceutical Sciences, 2007
Dhaka Univ. J. Pharm. Sci. Vol.4(1) 2005 The full text is of this article is available at the Dha... more Dhaka Univ. J. Pharm. Sci. Vol.4(1) 2005 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website
Bangladesh Pharmaceutical Journal
Traditional medicines including Ayurveda, Unani have been used as therapeutics in the Indian subc... more Traditional medicines including Ayurveda, Unani have been used as therapeutics in the Indian subcontinent since 5000 BC. Because of the abundance of secondary metabolites, medicinal plants are becoming priceless natural resources for better drug development. Till date almost no ethnopharmacological evidences were available on Colocasia genus except Colocasia esculenta. As a part of our continuing assessment of evidence based use of traditional medicine, we have identified bioactive polyphenols in the ethanolic leaves extract of Colocasia affinis Schott using HPLC-DAD method and evaluated its anti-allergy, anti-inflammatory and anti-diabetic potentials for the first time. Chromatographic investigation showed the presence of p-coumaric acid, trans-ferulic acid, rosmarinic acid, myricetin and kaempferol in the leaves extract. Pharmacological profile exploration demonstrated its anti-inflammatory activity at a dose of 250- and 500-mg/kg in xylene-induced ear edema in mice. The extract s...
Clinical Phytoscience
Background: The root-bark of the medicinally important plant Zanthoxylum budrunga (ZBRB) brooks a... more Background: The root-bark of the medicinally important plant Zanthoxylum budrunga (ZBRB) brooks a variety of uses in ethnobotanical and ethnomedicinal practice in Bangladesh and thus demands biological investigation to reveal its therapeutic potentiality. So, the present study was perpetrated to explore antioxidant, analgesic, antidiarrhoeal, and a cytotoxic activity of ethanolic root-bark extract of Zanthoxylum budrunga and was also to quantify the major bioactive polyphenolic constituents by HPLC analysis. Methods: Total phenolic content was measured spectrophotometrically by using Folin Chiocalteu's reagent while in vitro antioxidant activity was determined by means of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and reducing power assay. HPLC analysis was performed to identify and quantify the major bioactive polyphenolic constituents present in the extract. Acetic acid induced writhing test and hot-plate test were conducted to evaluate the analgesic activity of the crude extract. On the other hand, in vivo antidiarrheal potentiality was investigated by using experimentally castor oil induced diarrhoea in mice and brine shrimp lethality bio-assay was implemented to check the cytotoxic potentiality of the crude extract. Results: Zanthoxylum budrunga showed DPPH scavenging activity (IC 50 54.27 μg/mL), while the total phenolic content was 647.91 mg GAE/100 g of extract. ZBRB also showed concentration dependent ferric reducing power activity. At the doses of 250 and 500 mg/kg, ZBRB exhibited statistically significant (P < 0.001) inhibition of writhing in test mice (64.58 and 77.78%, respectively). In hot-plate test, ZBRB, at the above dose levels, significantly (P < 0.001) prolonged pain threshold with response time of 5.80 and 6.81 s, respectively. In castor oil induced diarrhoeal episode in mice, ZBRB exhibited 66.56% and 83.39% inhibition of defecation at the doses of 250 and 500 mg/kg, respectively (P < 0.001). The LC 50 (Median lethal concentration) value of ZBRB in brine shrimp lethality bioassay was found to be 21. 84 μg/mL. In the HPLC analysis, (+)-catechin, caffeic acid and quercetin were detected with the concentration of 17. 94 mg/100, 3.72 mg/100 and 11.95 mg/100 g of ethanolic extract of ZBRB, respectively. Conclusion: The results rationalize the uses of the plant in traditional medicine for diarrhoeal as well as pain management. Catechin, caffeic acid, and other phenolics constituents might have some function in the observed activity.
Clinical Phytoscience
Background: Acanthus ilicifolius L. is admired for its traditional usage in the folk medicine for... more Background: Acanthus ilicifolius L. is admired for its traditional usage in the folk medicine for the treatment of numerous diseases including allergy and helminthiasis in various parts of the planet. The ethanolic extract of the aerial parts of this shrub (EEAI) was investigated in the present study for its major phytochemical groups, antiallergic activity, anthelmintic activity, cytotoxicity and for acute toxicity. Methods: Antiallergic activity was carried out using Toluene 2, 4-diisocyanate (TDI)-induced allergic mice model by assessing various symptoms of allergic rhinitis like sneezing, scratching, swelling and watery rhinorrhea as well as counting the total and differential leukocytes profile of blood. The paralysis and death time of parasites, Haemonchus contortus (Nematoda) and Paramphistomum cervi (Trematoda) were used for anthelmintic activity test. Mortality of mice was counted to evaluate the acute toxicity whereas the mortality of brine shrimp was taken into account to assess cytotoxic potential of the extract. Results: Phytochemical screening of the extract demonstrated the presence of alkaloids, phenolic compounds, tannins, flavonoids, glycosides, saponins, steroids and triterpenoids. Oral pretreatment of the extract significantly ameliorated the TDI-induced allergic symptoms like sneezing (p < 0.05), scratching (p < 0.05), swelling and watery rhinorrhea in experimental mice. The extract also reduced the differential count of leukocytes in the blood which was increased due to induction of allergic conditions through TDI sensitization. In anthelmintic activity test the extract revealed a dose dependent decrease in the relative index of paralysis and death for both H. contortus and P. cervi parasites and thus indicated the extract to be parasiticidal at higher concentrations. In brine shrimp lethality bioassay of toxicity assessment, the LC 50 of the standard drug vincristine sulfate was 0.43 μg/mL whereas the extract showed the LC 50 as 44.57 μg/mL indicating a promising cytotoxicity of the extract. In acute toxicity study the highest dose 3 g/kg failed to show any mortality in Swiss albino mice and thus confirmed the safety of the extract for in vivo administration. Conclusions: The present study corroborated the traditional uses of the aerial parts of Acanthus ilicifolius L. in allergic diseases and in helminthiasis.
Australian Journal of Chemistry
Gram-negative bacteria such as Pseudomonas aeruginosa use furanosyl diesters as autoinducers for ... more Gram-negative bacteria such as Pseudomonas aeruginosa use furanosyl diesters as autoinducers for quorum sensing (QS), a major regulatory and cell-to-cell communication system for social adaptation, virulence factor production, biofilm formation, and antibiotic resistance. A range of natural and synthetic brominated furanones, i.e. fimbrolide derivatives, have been found to act as inhibitors of QS-dependent bacterial phenotypes, complementing the bactericidal ability of traditional antibiotics. In this work, several novel acetylene analogues of fimbrolides were synthesised in moderate to high yields via Sonogashira coupling reactions of brominated furanones 4-bromo-5-(bromomethylene)furan-2(5H)-one 4 and 5-(dibromomethylene)-3-ethylfuran-2(5H)-one 5. The Sonogashira reaction of acetylenes on 4-bromo-5-(bromomethylene)furan-2(5H)-one 4 was favoured at the C5 methylene bromide over the C4 bromide substituent. On biological testing, the most potent compounds 13 and 14 showed 82 and 98 %...