Ali Zirakzadeh | Karolinska Institutet (original) (raw)

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Papers by Ali Zirakzadeh

Research paper thumbnail of Beta-defensin production by human colonic plasma cells: a new look at plasma cells in ulcerative colitis

Inflammatory bowel diseases, 2007

Previously, we showed that colonic epithelium of ulcerative colitis (UC) patients expresses incre... more Previously, we showed that colonic epithelium of ulcerative colitis (UC) patients expresses increased levels of mRNA for 3 antimicrobial peptides, human beta-defensin 2 (hBD-2), hBD-3, and hBD-4 compared to controls. Human colon mucosa was analyzed using double immunofluorescence staining, in situ hybridization, immunoelectron microscopy, and quantitative real-time reverse-transcriptase polymerase chain reaction (qRT-PCR) with specific antibodies and probes in the respective assays. We demonstrate that lamina propria in colon from UC patients, Crohn's colitis patients, and controls contain cells that express hBD-2. These cells were identified as mature plasma cells by the highly specific CD138 marker, by their prominent IgA or IgG expression, and by their ultrastructural characteristics. By immunoelectron microscopy it was furthermore shown that the hBD-2 peptide was expressed in rough endoplasmic reticulum, the Golgi complex, and cytoplasmic vesicles, reflecting consecutive ste...

Research paper thumbnail of β-Defensin production by human colonic plasma cells: A new look at plasma cells in ulcerative colitis

Inflammatory Bowel Diseases, 2007

Background: Previously, we showed that colonic epithelium of ulcerative colitis (UC) patients exp... more Background: Previously, we showed that colonic epithelium of ulcerative colitis (UC) patients expresses increased levels of mRNA for 3 antimicrobial peptides, human ␤-defensin 2 (hBD-2), hBD-3, and hBD-4 compared to controls.

Research paper thumbnail of Response to comment on "multiplex B cell characterization in blood, lymph nodes, and tumors from patients with malignancies

Journal of immunology (Baltimore, Md. : 1950), 2013

Research paper thumbnail of Beta-defensin production by human colonic plasma cells: a new look at plasma cells in ulcerative colitis

Inflammatory bowel diseases, 2007

Previously, we showed that colonic epithelium of ulcerative colitis (UC) patients expresses incre... more Previously, we showed that colonic epithelium of ulcerative colitis (UC) patients expresses increased levels of mRNA for 3 antimicrobial peptides, human beta-defensin 2 (hBD-2), hBD-3, and hBD-4 compared to controls. Human colon mucosa was analyzed using double immunofluorescence staining, in situ hybridization, immunoelectron microscopy, and quantitative real-time reverse-transcriptase polymerase chain reaction (qRT-PCR) with specific antibodies and probes in the respective assays. We demonstrate that lamina propria in colon from UC patients, Crohn's colitis patients, and controls contain cells that express hBD-2. These cells were identified as mature plasma cells by the highly specific CD138 marker, by their prominent IgA or IgG expression, and by their ultrastructural characteristics. By immunoelectron microscopy it was furthermore shown that the hBD-2 peptide was expressed in rough endoplasmic reticulum, the Golgi complex, and cytoplasmic vesicles, reflecting consecutive ste...

Research paper thumbnail of β-Defensin production by human colonic plasma cells: A new look at plasma cells in ulcerative colitis

Inflammatory Bowel Diseases, 2007

Background: Previously, we showed that colonic epithelium of ulcerative colitis (UC) patients exp... more Background: Previously, we showed that colonic epithelium of ulcerative colitis (UC) patients expresses increased levels of mRNA for 3 antimicrobial peptides, human ␤-defensin 2 (hBD-2), hBD-3, and hBD-4 compared to controls.

Research paper thumbnail of Response to comment on "multiplex B cell characterization in blood, lymph nodes, and tumors from patients with malignancies

Journal of immunology (Baltimore, Md. : 1950), 2013

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