Suresh Verma | KIIT - Academia.edu (original) (raw)

Papers by Suresh Verma

Journal of Functional Biomaterials

Nanoscience has emerged as a fascinating field of science, with its implementation in multiple ap... more Nanoscience has emerged as a fascinating field of science, with its implementation in multiple applications in the form of nanotechnology. Nanotechnology has recently been more impactful in diverse sectors such as the pharmaceutical industry, agriculture sector, and food market. The peculiar properties which make nanoparticles as an asset are their large surface area and their size, which ranges between 1 and 100 nanometers (nm). Various technologies, such as chemical and biological processes, are being used to synthesize nanoparticles. The green chemistry route has become extremely popular due to its use in the synthesis of nanoparticles. Nanomaterials are versatile and impactful in different day to day applications, resulting in their increased utilization and distribution in human cells, tissues, and organs. Owing to the deployment of nanoparticles at a high demand, the need to produce nanoparticles has raised concerns regarding environmentally friendly processes. These processes...

Journal of Nanobiotechnology

High-quality point-of-care is critical for timely decision of disease diagnosis and healthcare ma... more High-quality point-of-care is critical for timely decision of disease diagnosis and healthcare management. In this regard, biosensors have revolutionized the field of rapid testing and screening, however, are confounded by several technical challenges including material cost, half-life, stability, site-specific targeting, analytes specificity, and detection sensitivity that affect the overall diagnostic potential and therapeutic profile. Despite their advances in point-of-care testing, very few classical biosensors have proven effective and commercially viable in situations of healthcare emergency including the recent COVID-19 pandemic. To overcome these challenges functionalized magnetic nanoparticles (MNPs) have emerged as key players in advancing the biomedical and healthcare sector with promising applications during the ongoing healthcare crises. This critical review focus on understanding recent developments in theranostic applications of functionalized magnetic nanoparticles (...

Green Chemistry, 2021

Molecular biocompatibility of AgNP is mediated through channelized intrinsic AgNP-proteins intera... more Molecular biocompatibility of AgNP is mediated through channelized intrinsic AgNP-proteins interaction in steatosis and apoptosis.

Journal of Nanotheranostics, 2021

The utility of calcium oxide nanoparticles in the biomedical and physical fields has instigated t... more The utility of calcium oxide nanoparticles in the biomedical and physical fields has instigated their biocompatible synthesis and production. Moreover, it is important to investigate their biocompatibility at the molecular level for biomedical and ecotoxicological concern. This study explores the green synthesis of calcium oxide nanoparticles (CaONP) using Crescentia cujete leaf extract. The synthesized CaONP were found to have a size of 62 ± 06 nm and a hydrodynamic diameter of 246 ± 12 nm, as determined by FE-SEM and dynamic light scattering (DLS). CaONP was stable in fish medium with a zeta potential of −23 ± 11 mV. The biocompatibility of the CaONP was investigated with adult zebrafish bearing an LC50 of 86.32 µg/mL. Cellular and molecular investigation revealed the mechanism of biocompatibility as a consequence of elicited reactive oxygen species leading to apoptosis, due to accumulation and internalization of CaONP in exposed zebrafish. The study provided detailed information ...

Artificial cells, nanomedicine, and biotechnology, Jan 12, 2018

Implication of gold nanoparticles in industrial and day-to-day life products at extensive scale h... more Implication of gold nanoparticles in industrial and day-to-day life products at extensive scale has raised concern about their toxicity to environment and human health. Moreover, quest of new technologies for production of biocompatible nanoparticles increased. This study explores the molecular toxicology of AuNP with enlightenment of their green synthesis using medicinal plant extract as reducing and stabilizing agent. Synthesized CAuNP were characterized for their physiochemical properties by standard techniques like FESEM, TEM, DLS, UV-Vis spectroscopy and FTIR. GCMS analysis revealed the involvement of -OH compounds for CAuNP synthesis. Determined size and zeta potential of CAuNP was found to be 21 ± 08 nm and -24 ± 11 mV with SPR peak at 554 nm. LC50 of CAuNP with zebrafish embryos was 69 ± 12 µg/ml compared to 52 ± 06 µg/ml of AuNP. Gold nanoparticles were found to exhibit concentration dependent morphological abnormalities with acute effect at cellular and molecular level. Ex...

Scientific reports, Jan 24, 2017

With the rapid development of nanotechnology, much has been anticipated with copper oxide nanopar... more With the rapid development of nanotechnology, much has been anticipated with copper oxide nanoparticles (CuO NP) due to their extensive industrial and commercial application. However, it has raised concern over the environmental safety and human health effects. In this study, CuO nanoparticles were synthesized using the green method with floral extract of Calotropis gigantea and characterized by standard physiochemical techniques like DLS, Zeta potential determination, UV- Visible Spectroscopy, XRD, FTIR, FESEM, and TEM. Mechanistic cytotoxicity studies were performed using experimental and computational assays including morphological analysis, hatching, and viability rate analysis along with ROS and apoptosis analysis. Physiochemical characterization of CuO NP determined the size and zeta potential of synthesized nanoparticles to be 30 ± 09 nm to 40 ± 2 nm and -38 mV ± 12 mV respectively. Cytotoxicity evaluation with Zebrafish revealed malfunctioned organ development with different...

Precision Nanomedicine, 2021

With the advancement of technologies, the evolution of nanoscience is occurring at a rapid pace. ... more With the advancement of technologies, the evolution of nanoscience is occurring at a rapid pace. The demand for nanomaterials in various industries has increased. However, they may pose an increased risk upon exposure to living organisms, demanding toxicological studies. Alt-hough risk assessment of nanomaterials is a challenge due to their varieties in sizes and variabil-ity in properties, different methodologies of toxicity screenings have been used successfully to evaluate the potential risks. Although nanotechnology has contributed to technology develop-ment and well-being, information in these fields is still incomplete. Fortunately, those areas are under continuous research, and there are increasing experimental efforts in determining the envi-ronmental effects on humans exposed to nanoparticles. Since the field of nanotechnology is continuously expanding, we need medium- to high throughput nanotoxicity screenings to deter-mine any potential risks caused by nanomaterials. Zebr...

The present study was conducted to evaluate the effect of dietary inclusion of silver nanoparticl... more The present study was conducted to evaluate the effect of dietary inclusion of silver nanoparticle on general performance and digestive proteases in zebra fish. Four experimental diets were designed with a concentration of 5, 20 and 40 parts per billion (ppb) nanoparticles in the diet (named S5, S20, and S40, respectively) and control group without any nanoparticles (S0). There was no significant difference was observed in FI and FCR between the dietary treatments. At the end of the experiment, S20 exhibited highest WG and SGR followed by S40 compared with other treatments while, there was no significant difference observed between S0 and S5. Similar trends were also observed in total protease enzyme activity. To evaluate the protease enzyme patterns on gut extract, substrate SDS-PAGE was performed and the inhibition of zymogram was studied. The results showed that there was no difference in banding patterns between S0 and S5 with EDTA treated samples whereas two extra bands of mole...

Journal of Functional Biomaterials

Nanoscience has emerged as a fascinating field of science, with its implementation in multiple ap... more Nanoscience has emerged as a fascinating field of science, with its implementation in multiple applications in the form of nanotechnology. Nanotechnology has recently been more impactful in diverse sectors such as the pharmaceutical industry, agriculture sector, and food market. The peculiar properties which make nanoparticles as an asset are their large surface area and their size, which ranges between 1 and 100 nanometers (nm). Various technologies, such as chemical and biological processes, are being used to synthesize nanoparticles. The green chemistry route has become extremely popular due to its use in the synthesis of nanoparticles. Nanomaterials are versatile and impactful in different day to day applications, resulting in their increased utilization and distribution in human cells, tissues, and organs. Owing to the deployment of nanoparticles at a high demand, the need to produce nanoparticles has raised concerns regarding environmentally friendly processes. These processes...

Journal of Nanobiotechnology

High-quality point-of-care is critical for timely decision of disease diagnosis and healthcare ma... more High-quality point-of-care is critical for timely decision of disease diagnosis and healthcare management. In this regard, biosensors have revolutionized the field of rapid testing and screening, however, are confounded by several technical challenges including material cost, half-life, stability, site-specific targeting, analytes specificity, and detection sensitivity that affect the overall diagnostic potential and therapeutic profile. Despite their advances in point-of-care testing, very few classical biosensors have proven effective and commercially viable in situations of healthcare emergency including the recent COVID-19 pandemic. To overcome these challenges functionalized magnetic nanoparticles (MNPs) have emerged as key players in advancing the biomedical and healthcare sector with promising applications during the ongoing healthcare crises. This critical review focus on understanding recent developments in theranostic applications of functionalized magnetic nanoparticles (...

Green Chemistry, 2021

Molecular biocompatibility of AgNP is mediated through channelized intrinsic AgNP-proteins intera... more Molecular biocompatibility of AgNP is mediated through channelized intrinsic AgNP-proteins interaction in steatosis and apoptosis.

Journal of Nanotheranostics, 2021

The utility of calcium oxide nanoparticles in the biomedical and physical fields has instigated t... more The utility of calcium oxide nanoparticles in the biomedical and physical fields has instigated their biocompatible synthesis and production. Moreover, it is important to investigate their biocompatibility at the molecular level for biomedical and ecotoxicological concern. This study explores the green synthesis of calcium oxide nanoparticles (CaONP) using Crescentia cujete leaf extract. The synthesized CaONP were found to have a size of 62 ± 06 nm and a hydrodynamic diameter of 246 ± 12 nm, as determined by FE-SEM and dynamic light scattering (DLS). CaONP was stable in fish medium with a zeta potential of −23 ± 11 mV. The biocompatibility of the CaONP was investigated with adult zebrafish bearing an LC50 of 86.32 µg/mL. Cellular and molecular investigation revealed the mechanism of biocompatibility as a consequence of elicited reactive oxygen species leading to apoptosis, due to accumulation and internalization of CaONP in exposed zebrafish. The study provided detailed information ...

Artificial cells, nanomedicine, and biotechnology, Jan 12, 2018

Implication of gold nanoparticles in industrial and day-to-day life products at extensive scale h... more Implication of gold nanoparticles in industrial and day-to-day life products at extensive scale has raised concern about their toxicity to environment and human health. Moreover, quest of new technologies for production of biocompatible nanoparticles increased. This study explores the molecular toxicology of AuNP with enlightenment of their green synthesis using medicinal plant extract as reducing and stabilizing agent. Synthesized CAuNP were characterized for their physiochemical properties by standard techniques like FESEM, TEM, DLS, UV-Vis spectroscopy and FTIR. GCMS analysis revealed the involvement of -OH compounds for CAuNP synthesis. Determined size and zeta potential of CAuNP was found to be 21 ± 08 nm and -24 ± 11 mV with SPR peak at 554 nm. LC50 of CAuNP with zebrafish embryos was 69 ± 12 µg/ml compared to 52 ± 06 µg/ml of AuNP. Gold nanoparticles were found to exhibit concentration dependent morphological abnormalities with acute effect at cellular and molecular level. Ex...

Scientific reports, Jan 24, 2017

With the rapid development of nanotechnology, much has been anticipated with copper oxide nanopar... more With the rapid development of nanotechnology, much has been anticipated with copper oxide nanoparticles (CuO NP) due to their extensive industrial and commercial application. However, it has raised concern over the environmental safety and human health effects. In this study, CuO nanoparticles were synthesized using the green method with floral extract of Calotropis gigantea and characterized by standard physiochemical techniques like DLS, Zeta potential determination, UV- Visible Spectroscopy, XRD, FTIR, FESEM, and TEM. Mechanistic cytotoxicity studies were performed using experimental and computational assays including morphological analysis, hatching, and viability rate analysis along with ROS and apoptosis analysis. Physiochemical characterization of CuO NP determined the size and zeta potential of synthesized nanoparticles to be 30 ± 09 nm to 40 ± 2 nm and -38 mV ± 12 mV respectively. Cytotoxicity evaluation with Zebrafish revealed malfunctioned organ development with different...

Precision Nanomedicine, 2021

With the advancement of technologies, the evolution of nanoscience is occurring at a rapid pace. ... more With the advancement of technologies, the evolution of nanoscience is occurring at a rapid pace. The demand for nanomaterials in various industries has increased. However, they may pose an increased risk upon exposure to living organisms, demanding toxicological studies. Alt-hough risk assessment of nanomaterials is a challenge due to their varieties in sizes and variabil-ity in properties, different methodologies of toxicity screenings have been used successfully to evaluate the potential risks. Although nanotechnology has contributed to technology develop-ment and well-being, information in these fields is still incomplete. Fortunately, those areas are under continuous research, and there are increasing experimental efforts in determining the envi-ronmental effects on humans exposed to nanoparticles. Since the field of nanotechnology is continuously expanding, we need medium- to high throughput nanotoxicity screenings to deter-mine any potential risks caused by nanomaterials. Zebr...

The present study was conducted to evaluate the effect of dietary inclusion of silver nanoparticl... more The present study was conducted to evaluate the effect of dietary inclusion of silver nanoparticle on general performance and digestive proteases in zebra fish. Four experimental diets were designed with a concentration of 5, 20 and 40 parts per billion (ppb) nanoparticles in the diet (named S5, S20, and S40, respectively) and control group without any nanoparticles (S0). There was no significant difference was observed in FI and FCR between the dietary treatments. At the end of the experiment, S20 exhibited highest WG and SGR followed by S40 compared with other treatments while, there was no significant difference observed between S0 and S5. Similar trends were also observed in total protease enzyme activity. To evaluate the protease enzyme patterns on gut extract, substrate SDS-PAGE was performed and the inhibition of zymogram was studied. The results showed that there was no difference in banding patterns between S0 and S5 with EDTA treated samples whereas two extra bands of mole...