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Burapha Science Journal (วารสารวิทยาศาสตร์บูรพา ), Jun 1, 2021
The essential oil from guava leaves has low physicochemical stability and low solubility in water... more The essential oil from guava leaves has low physicochemical stability and low solubility in water, what limits its application in food formulations. This study aimed to characterize the physicochemical properties and the antioxidant and antimicrobial activities of encapsulated guava leaf oil in hydroxypropyl-β-cyclodextrin (HPβCD). Inclusion complex formation of guava leaf oil and HPβCD was determined by several techniques. Antioxidant activity of encapsulated guava leaf oil was more stable to sunlight exposure than free guava leaf oil by 26-38%. The antibacterial activity of guava leaf oil against Staphylococcus aureus and Escherichia coli was improved by 4 and 2 times after encapsulation in HPβCD, respectively.
The Thai Journal of Veterinary Medicine
Veterinary World
Background and Aim: Mastitis is an essential issue in dairy cows. Post-milking teat dips can help... more Background and Aim: Mastitis is an essential issue in dairy cows. Post-milking teat dips can help reduce this problem, but they employ harsh disinfectants, and many bacteria are becoming increasingly tolerant. This study aimed to investigate the antibacterial activity of clove, citronella, and sweet basil essential oils against the common bovine mastitis causative agents Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli and to develop an antiseptic post-milking teat spray for use in dairy cows. Materials and Methods: The in vitro antimicrobial activity of the essential oils was determined by broth microdilution and time-kill assays. Essential oil-based post-milking teat sprays were developed. The bacterial eradication efficacy of the formulations was determined by time-kill assays and their stability was tested by repeated freeze-thaw cycles. The most effective formulation was tested in dairy cows. Results: The minimum inhibitory concentrations and minimum bacter...
Veterinary World
Background and Aim: Microsporum gallinae is the major dermatophyte species that causes avian derm... more Background and Aim: Microsporum gallinae is the major dermatophyte species that causes avian dermatophytosis. Disinfection plays an important role in controlling and preventing dermatophytosis; however, information about the effect of common disinfection processes on M. gallinae is limited. This study aimed to investigate the disinfection efficacy of ultraviolet (UV) irradiation, heat treatment, detergents, and germicides against infective spores (arthroconidia) and vegetative mycelia of M. gallinae. Materials and Methods: The minimum inhibitory and minimum fungicidal concentrations of benzalkonium chloride, chlorhexidine, ethanol, formaldehyde, glutaraldehyde, hydrogen peroxide, phenol, povidone-iodine, and sodium hypochlorite germicides against arthroconidia and mycelia of M. gallinae American type culture collection (ATCC) 90749 were determined by broth microdilution. Time-kill assays were used to determine the fungicidal efficacy of moist heat treatment, UV irradiation, commerci...
ScienceAsia, 2020
The aim of this study was to investigate the efficacy of 11 essential oils (EOs) against Staphylo... more The aim of this study was to investigate the efficacy of 11 essential oils (EOs) against Staphylococcus pseudintermedius planktonic and biofilm forms. Twenty three bacterial samples were collected from dogs with superficial pyoderma and 9 samples were identified as S. pseudintermedius by polymerase chain reaction-restriction fragment length polymorphism method. The minimum planktonic inhibitory concentration (MPIC), minimum planktonic bactericidal concentration (MPBC) and minimum biofilm eradication concentration (MBEC) of EOs were determined by broth microdilution using a transferable solid phase. Among all, lemongrass EO was the most effective against both planktonic and biofilm forms of S. pseudintermedius with the MPIC, MPBC and MBEC of 0.02, 0.039 and 0.078% v/v, respectively. EOs of betel vine and citronella had the second most inhibitory effect (MPIC and MPBC: 0.039% v/v and MPBC: 0.156% v/v) followed by clove (MPIC and MPBC: 0.078% v/v) and sweet basil (MPIC and MPBC: 0.625% v/v). The EOs of ginger, plai, kaffir lime, turmeric, holy basil and galanga had the lowest inhibitory effect with MPICs and MPBCs ranging from 1.25% v/v to greater than 2.5% v/v. The results of this study indicated that the anti-planktonic and anti-biofilm effects of the tested EOs were concentration dependent, and higher concentrations were required for anti-biofilm activity. Lemongrass EO showed the most potential as a therapeutic antimicrobial agent for both planktonic and biofilm forms of S. pseudintermedius.
Chiang Mai University Journal of Natural Sciences, 2020
The bacteria causing mastitis in goats reduce the quality of milk products resulting in economic ... more The bacteria causing mastitis in goats reduce the quality of milk products resulting in economic losses and the use of antiseptic agents leave residues in the milk. Therefore, this study investigated the antibacterial activity of lemongrass, kaffir lime and holy basil essential oils against subclinical mastitis bacterial isolates from dairy goats. A total of 47 bacteria were isolated, consisting of 40 staphylococci (34 coagulase-negative staphylococci (CNS), 5 S. aureus and 1 S. epidermidis), 4 α-Streptococcus spp., 2 Bacillus spp. and 1 Alcaligenes faecalis. The main components of essential oil of lemongrass were geranial, neral, myrcene and geraniol (45.32-4.12%), kaffir lime consisted of β-pinene, DLlimonene, terpinene-4-ol and α-terpineol (25.58-12.24%) and holy basil consisted of methyl eugenol, caryophyllene and eugenol (52.43-8.68%). Lemongrass essential oil showed the highest antibacterial activity against CNS and S. aureus with average inhibition zone diameters of 25.00±1.7...
The bacteria causing mastitis in goats reduce the quality of milk products resulting in economic ... more The bacteria causing mastitis in goats reduce the quality of milk products resulting in economic losses and the use of antiseptic agents leave residues in the milk. Therefore, this study investigated the antibacterial activity of lemongrass, kaffir lime and holy basil essential oils against subclinical mastitis bacterial isolates from dairy goats. A total of 47 bacteria were isolated, consisting of 40 staphylococci (34 coagulase-negative staphylococci (CNS), 5 S. aureus and 1 S. epidermidis), 4 α-Streptococcus spp., 2 Bacillus spp. and 1 Alcaligenes faecalis. The main components of essential oil of lemongrass were geranial, neral, myrcene and geraniol (45.32-4.12%), kaffir lime consisted of β-pinene, DLlimonene, terpinene-4-ol and α-terpineol (25.58-12.24%) and holy basil consisted of methyl eugenol, caryophyllene and eugenol (52.43-8.68%). Lemongrass essential oil showed the highest antibacterial activity against CNS and S. aureus with average inhibition zone diameters of 25.00±1.7...
Burapha Science Journal (วารสารวิทยาศาสตร์บูรพา ), Jun 1, 2021
The essential oil from guava leaves has low physicochemical stability and low solubility in water... more The essential oil from guava leaves has low physicochemical stability and low solubility in water, what limits its application in food formulations. This study aimed to characterize the physicochemical properties and the antioxidant and antimicrobial activities of encapsulated guava leaf oil in hydroxypropyl-β-cyclodextrin (HPβCD). Inclusion complex formation of guava leaf oil and HPβCD was determined by several techniques. Antioxidant activity of encapsulated guava leaf oil was more stable to sunlight exposure than free guava leaf oil by 26-38%. The antibacterial activity of guava leaf oil against Staphylococcus aureus and Escherichia coli was improved by 4 and 2 times after encapsulation in HPβCD, respectively.
The Thai Journal of Veterinary Medicine
Veterinary World
Background and Aim: Mastitis is an essential issue in dairy cows. Post-milking teat dips can help... more Background and Aim: Mastitis is an essential issue in dairy cows. Post-milking teat dips can help reduce this problem, but they employ harsh disinfectants, and many bacteria are becoming increasingly tolerant. This study aimed to investigate the antibacterial activity of clove, citronella, and sweet basil essential oils against the common bovine mastitis causative agents Staphylococcus aureus, Streptococcus agalactiae, and Escherichia coli and to develop an antiseptic post-milking teat spray for use in dairy cows. Materials and Methods: The in vitro antimicrobial activity of the essential oils was determined by broth microdilution and time-kill assays. Essential oil-based post-milking teat sprays were developed. The bacterial eradication efficacy of the formulations was determined by time-kill assays and their stability was tested by repeated freeze-thaw cycles. The most effective formulation was tested in dairy cows. Results: The minimum inhibitory concentrations and minimum bacter...
Veterinary World
Background and Aim: Microsporum gallinae is the major dermatophyte species that causes avian derm... more Background and Aim: Microsporum gallinae is the major dermatophyte species that causes avian dermatophytosis. Disinfection plays an important role in controlling and preventing dermatophytosis; however, information about the effect of common disinfection processes on M. gallinae is limited. This study aimed to investigate the disinfection efficacy of ultraviolet (UV) irradiation, heat treatment, detergents, and germicides against infective spores (arthroconidia) and vegetative mycelia of M. gallinae. Materials and Methods: The minimum inhibitory and minimum fungicidal concentrations of benzalkonium chloride, chlorhexidine, ethanol, formaldehyde, glutaraldehyde, hydrogen peroxide, phenol, povidone-iodine, and sodium hypochlorite germicides against arthroconidia and mycelia of M. gallinae American type culture collection (ATCC) 90749 were determined by broth microdilution. Time-kill assays were used to determine the fungicidal efficacy of moist heat treatment, UV irradiation, commerci...
ScienceAsia, 2020
The aim of this study was to investigate the efficacy of 11 essential oils (EOs) against Staphylo... more The aim of this study was to investigate the efficacy of 11 essential oils (EOs) against Staphylococcus pseudintermedius planktonic and biofilm forms. Twenty three bacterial samples were collected from dogs with superficial pyoderma and 9 samples were identified as S. pseudintermedius by polymerase chain reaction-restriction fragment length polymorphism method. The minimum planktonic inhibitory concentration (MPIC), minimum planktonic bactericidal concentration (MPBC) and minimum biofilm eradication concentration (MBEC) of EOs were determined by broth microdilution using a transferable solid phase. Among all, lemongrass EO was the most effective against both planktonic and biofilm forms of S. pseudintermedius with the MPIC, MPBC and MBEC of 0.02, 0.039 and 0.078% v/v, respectively. EOs of betel vine and citronella had the second most inhibitory effect (MPIC and MPBC: 0.039% v/v and MPBC: 0.156% v/v) followed by clove (MPIC and MPBC: 0.078% v/v) and sweet basil (MPIC and MPBC: 0.625% v/v). The EOs of ginger, plai, kaffir lime, turmeric, holy basil and galanga had the lowest inhibitory effect with MPICs and MPBCs ranging from 1.25% v/v to greater than 2.5% v/v. The results of this study indicated that the anti-planktonic and anti-biofilm effects of the tested EOs were concentration dependent, and higher concentrations were required for anti-biofilm activity. Lemongrass EO showed the most potential as a therapeutic antimicrobial agent for both planktonic and biofilm forms of S. pseudintermedius.
Chiang Mai University Journal of Natural Sciences, 2020
The bacteria causing mastitis in goats reduce the quality of milk products resulting in economic ... more The bacteria causing mastitis in goats reduce the quality of milk products resulting in economic losses and the use of antiseptic agents leave residues in the milk. Therefore, this study investigated the antibacterial activity of lemongrass, kaffir lime and holy basil essential oils against subclinical mastitis bacterial isolates from dairy goats. A total of 47 bacteria were isolated, consisting of 40 staphylococci (34 coagulase-negative staphylococci (CNS), 5 S. aureus and 1 S. epidermidis), 4 α-Streptococcus spp., 2 Bacillus spp. and 1 Alcaligenes faecalis. The main components of essential oil of lemongrass were geranial, neral, myrcene and geraniol (45.32-4.12%), kaffir lime consisted of β-pinene, DLlimonene, terpinene-4-ol and α-terpineol (25.58-12.24%) and holy basil consisted of methyl eugenol, caryophyllene and eugenol (52.43-8.68%). Lemongrass essential oil showed the highest antibacterial activity against CNS and S. aureus with average inhibition zone diameters of 25.00±1.7...
The bacteria causing mastitis in goats reduce the quality of milk products resulting in economic ... more The bacteria causing mastitis in goats reduce the quality of milk products resulting in economic losses and the use of antiseptic agents leave residues in the milk. Therefore, this study investigated the antibacterial activity of lemongrass, kaffir lime and holy basil essential oils against subclinical mastitis bacterial isolates from dairy goats. A total of 47 bacteria were isolated, consisting of 40 staphylococci (34 coagulase-negative staphylococci (CNS), 5 S. aureus and 1 S. epidermidis), 4 α-Streptococcus spp., 2 Bacillus spp. and 1 Alcaligenes faecalis. The main components of essential oil of lemongrass were geranial, neral, myrcene and geraniol (45.32-4.12%), kaffir lime consisted of β-pinene, DLlimonene, terpinene-4-ol and α-terpineol (25.58-12.24%) and holy basil consisted of methyl eugenol, caryophyllene and eugenol (52.43-8.68%). Lemongrass essential oil showed the highest antibacterial activity against CNS and S. aureus with average inhibition zone diameters of 25.00±1.7...