A Syed Sameer | King Saud Bin Abdulaziz University for Health Sciences (original) (raw)

Papers by A Syed Sameer

Journal of Genetics and Molecular Biology, 2010

Colorectal cancer (CRC) being the commonest cancer, is the major cause of mortality and morbidity... more Colorectal cancer (CRC) being the commonest cancer, is the major cause of mortality and morbidity worldwide. TGF-β pathway is one of the important pathways that play a prominent role in cell proliferation, differentiation, migration and apoptosis. Smad dependent TGF-β signaling cascade is responsible for the regulation and expression of almost 500 odd genes, which in turn play important role in the proper development of intestinal mucosa. Here in this review we have discussed the overall machinery of theTGF-β pathway and the advances in the mutational research on SMAD4 gene in cancers with special look on our own research in CRC cases of Kashmiri population. Key words: Colorectal cancer, Kashmir, SMADs, mutations, PCR-SSCP.

Asian Pacific journal of cancer prevention: APJCP

ABSTRACT

International Journal of Pharmacy and Pharmaceutical Sciences

European Journal of Cancer Prevention, 2014

Saudi Journal of Gastroenterology, 2009

Open Colorectal Cancer Journal, 2011

Indian Journal of Clinical Biochemistry, 2010

Hypertension is the most important public health problem in developing countries and one of the m... more Hypertension is the most important public health problem in developing countries and one of the major risk factors for cardiovascular diseases, and it has been reported that hypertension is in part an inflammatory disorder and several workers have reported elevated levels of CRP in hypertensive individuals. The main aim of the present study was to evaluate the association between blood pressure and serum CRP levels across the range of blood pressure categories including prehypertension. A total of 104 patients and 63 control subjects were included in the present study. The level of CRP in the serum samples was estimated by a high sensitivity immunoturbidometric assay. Standard unpaired student's 't' test was used for comparison of hs-CRP levels between hypertensive patients and normotensive control subjects and between patient groups with different grades of hypertension and different durations of hypertensive histories. The mean serum hs-CRP level in hypertensive patients was 3.26 mg/L compared with 1.36 mg/L among normotensive control subjects (P<0.001). On comparison with normotensive control subjects, the hs-CRP levels vary significantly both with grades and duration of hypertension, with most significant difference found in patients with prehypertension (P<0.001), followed by Stage-I (P=0.01) and Stage-II(P=0.02) hypertensives. Significant difference in hs-CRP levels was also found in patients with shorter duration of hypertensive history (≤ 1year) when compared with those with ≥ 5 years of hypertensive history (P<0.01). Our study reveals a graded association between blood pressure and CRP elevation in people with hypertension. Individuals with prehypertension or with shorter duration of hypertension (≤1 Year) had significantly a greater likelihood of CRP elevation in comparison to chronic stage-I or stage-II hypertensives.

International Journal of Research in Engineering and Technology

Asian Pacific Journal of Cancer Prevention Apjcp, 2010

Background: The role of caveolae and the caveolin proteins in cancer has been the subject of exte... more Background: The role of caveolae and the caveolin proteins in cancer has been the subject of extensive research. It has been suggested that caveolin-1 (Cav-1) may contribute to certain steps of carcinogenesis. In the present study we focused on its potential clinical relevance in mammary malignancies. Methods: We investigated 130 breast cancer samples along with adjacent normal tissues using allele specific PCR for the mutation status and then conventional PCR-SSCP and sequencing of mutated samples along with the normal adjacent tissues. Results: Caveolin-1 was identified in a screen for genes involved in breast cancer progression and we demonstrated 29.2% mutational status in our Kashmiri ethnic population. We were able to detect 38 mutations out of which 22 were missense, 4 were nonsense, and 12 were frame shifts amongst these 38 we were also able to detect ten novel Cav-1 mutations (missense and frameshift mutations). Conclusion: We conclude that our study suggests that the gene encoding Cav-1 plays an important role in the promotion of mammary tumorigenesis and are associated with the development and progression of breast cancer.

Asian Pacific journal of cancer prevention: APJCP

Purpose: We made a preliminary attempt to study mutations in exons 5-8 (the DNA binding domain) o... more Purpose: We made a preliminary attempt to study mutations in exons 5-8 (the DNA binding domain) of the tumor suppressor gene TP53, in urinary bladder cancer patients from Kashmir. Further the relation of clinicopathological characteristics with mutation status was asessed. Materials and Methods: The study population consisted of 60 patients diagnosed with transitional cell carcinomas who underwent transurethral resection and /or radical cystectomy. Mutations in 5-8 exons of TP53 gene were detected by means of single strand conformation polymorphism (SSCP). All samples which showed different migration bands in SSCP were confirmed by DNA sequencing. Results: 19 of 60 (31.6%) bladder cancers had mutations of the TP53 gene (11 transitions and 8 transversions), three were G→A transitions, two G→T transversions, three A→C transversions, five C→T transitions and six A→T transversions. Predominantly missense mutations (66%) were detected but no deletions or insertions were found. Statistically significant associations (<0.05) were noted with higher tumor stage (T2 or higher), recurrence and large tumor size (>3cm). No correlation was found between smoking and tumor grade and the presence of TP53 mutations. Conclusions: Mutation of the TP53 gene is one of the commonest genetic changes in human bladder cancer, also in a high risk ethnic Kashmiri population.

Breast carcinoma has been considered a female dominated disease, whereas male breast carcinoma is... more Breast carcinoma has been considered a female dominated disease, whereas male breast carcinoma is relatively rare accounting for less than 1% of all breast cancers. Cancer of the breast among men is rare, accounting for less than one percent of cancer incidence and mortality in this sex group. We present the case of a 14-year-old boy with infiltrating ductal breast carcinoma; this is the fifth report of the world and the third from India.

Journal of Genetics and Molecular Biology, 2010

Colorectal cancer (CRC) being the commonest cancer, is the major cause of mortality and morbidity... more Colorectal cancer (CRC) being the commonest cancer, is the major cause of mortality and morbidity worldwide. TGF-β pathway is one of the important pathways that play a prominent role in cell proliferation, differentiation, migration and apoptosis. Smad dependent TGF-β signaling cascade is responsible for the regulation and expression of almost 500 odd genes, which in turn play important role in the proper development of intestinal mucosa. Here in this review we have discussed the overall machinery of theTGF-β pathway and the advances in the mutational research on SMAD4 gene in cancers with special look on our own research in CRC cases of Kashmiri population. Key words: Colorectal cancer, Kashmir, SMADs, mutations, PCR-SSCP.

Asian Pacific journal of cancer prevention: APJCP

ABSTRACT

International Journal of Pharmacy and Pharmaceutical Sciences

European Journal of Cancer Prevention, 2014

Saudi Journal of Gastroenterology, 2009

Open Colorectal Cancer Journal, 2011

Indian Journal of Clinical Biochemistry, 2010

Hypertension is the most important public health problem in developing countries and one of the m... more Hypertension is the most important public health problem in developing countries and one of the major risk factors for cardiovascular diseases, and it has been reported that hypertension is in part an inflammatory disorder and several workers have reported elevated levels of CRP in hypertensive individuals. The main aim of the present study was to evaluate the association between blood pressure and serum CRP levels across the range of blood pressure categories including prehypertension. A total of 104 patients and 63 control subjects were included in the present study. The level of CRP in the serum samples was estimated by a high sensitivity immunoturbidometric assay. Standard unpaired student's 't' test was used for comparison of hs-CRP levels between hypertensive patients and normotensive control subjects and between patient groups with different grades of hypertension and different durations of hypertensive histories. The mean serum hs-CRP level in hypertensive patients was 3.26 mg/L compared with 1.36 mg/L among normotensive control subjects (P<0.001). On comparison with normotensive control subjects, the hs-CRP levels vary significantly both with grades and duration of hypertension, with most significant difference found in patients with prehypertension (P<0.001), followed by Stage-I (P=0.01) and Stage-II(P=0.02) hypertensives. Significant difference in hs-CRP levels was also found in patients with shorter duration of hypertensive history (≤ 1year) when compared with those with ≥ 5 years of hypertensive history (P<0.01). Our study reveals a graded association between blood pressure and CRP elevation in people with hypertension. Individuals with prehypertension or with shorter duration of hypertension (≤1 Year) had significantly a greater likelihood of CRP elevation in comparison to chronic stage-I or stage-II hypertensives.

International Journal of Research in Engineering and Technology

Asian Pacific Journal of Cancer Prevention Apjcp, 2010

Background: The role of caveolae and the caveolin proteins in cancer has been the subject of exte... more Background: The role of caveolae and the caveolin proteins in cancer has been the subject of extensive research. It has been suggested that caveolin-1 (Cav-1) may contribute to certain steps of carcinogenesis. In the present study we focused on its potential clinical relevance in mammary malignancies. Methods: We investigated 130 breast cancer samples along with adjacent normal tissues using allele specific PCR for the mutation status and then conventional PCR-SSCP and sequencing of mutated samples along with the normal adjacent tissues. Results: Caveolin-1 was identified in a screen for genes involved in breast cancer progression and we demonstrated 29.2% mutational status in our Kashmiri ethnic population. We were able to detect 38 mutations out of which 22 were missense, 4 were nonsense, and 12 were frame shifts amongst these 38 we were also able to detect ten novel Cav-1 mutations (missense and frameshift mutations). Conclusion: We conclude that our study suggests that the gene encoding Cav-1 plays an important role in the promotion of mammary tumorigenesis and are associated with the development and progression of breast cancer.

Asian Pacific journal of cancer prevention: APJCP

Purpose: We made a preliminary attempt to study mutations in exons 5-8 (the DNA binding domain) o... more Purpose: We made a preliminary attempt to study mutations in exons 5-8 (the DNA binding domain) of the tumor suppressor gene TP53, in urinary bladder cancer patients from Kashmir. Further the relation of clinicopathological characteristics with mutation status was asessed. Materials and Methods: The study population consisted of 60 patients diagnosed with transitional cell carcinomas who underwent transurethral resection and /or radical cystectomy. Mutations in 5-8 exons of TP53 gene were detected by means of single strand conformation polymorphism (SSCP). All samples which showed different migration bands in SSCP were confirmed by DNA sequencing. Results: 19 of 60 (31.6%) bladder cancers had mutations of the TP53 gene (11 transitions and 8 transversions), three were G→A transitions, two G→T transversions, three A→C transversions, five C→T transitions and six A→T transversions. Predominantly missense mutations (66%) were detected but no deletions or insertions were found. Statistically significant associations (<0.05) were noted with higher tumor stage (T2 or higher), recurrence and large tumor size (>3cm). No correlation was found between smoking and tumor grade and the presence of TP53 mutations. Conclusions: Mutation of the TP53 gene is one of the commonest genetic changes in human bladder cancer, also in a high risk ethnic Kashmiri population.

Breast carcinoma has been considered a female dominated disease, whereas male breast carcinoma is... more Breast carcinoma has been considered a female dominated disease, whereas male breast carcinoma is relatively rare accounting for less than 1% of all breast cancers. Cancer of the breast among men is rare, accounting for less than one percent of cancer incidence and mortality in this sex group. We present the case of a 14-year-old boy with infiltrating ductal breast carcinoma; this is the fifth report of the world and the third from India.