Satpal Bisht | Kumaun University Nainital, Uttarakhand, India (original) (raw)

Papers by Satpal Bisht

Metabolites

The ability of microorganisms to detoxify xenobiotic compounds allows them to thrive in a toxic e... more The ability of microorganisms to detoxify xenobiotic compounds allows them to thrive in a toxic environment using carbon, phosphorus, sulfur, and nitrogen from the available sources. Biotransformation is the most effective and useful metabolic process to degrade xenobiotic compounds. Microorganisms have an exceptional ability due to particular genes, enzymes, and degradative mechanisms. Microorganisms such as bacteria and fungi have unique properties that enable them to partially or completely metabolize the xenobiotic substances in various ecosystems.There are many cutting-edge approaches available to understand the molecular mechanism of degradative processes and pathways to decontaminate or change the core structure of xenobiotics in nature. These methods examine microorganisms, their metabolic machinery, novel proteins, and catabolic genes. This article addresses recent advances and current trends to characterize the catabolic genes, enzymes and the techniques involved in combat...

ABSTRACT Twelve microbes were isolated from the metal scrap land fill site of Rourkela steel plan... more ABSTRACT Twelve microbes were isolated from the metal scrap land fill site of Rourkela steel plant (B1, B2, B3, B4, R1, R2, R3, R4, M1, M3, N2 and N3). Majority were positive for dextrose fermentation and catalase test. R3 had partial resistance, and M3 and N2 had high resistance against iron metal. Remaining were sensitive.

Additional file 1: Table S1. Top fifteen OTUâ s based on total read count number among the hot sp... more Additional file 1: Table S1. Top fifteen OTUâ s based on total read count number among the hot spring samples. Table S2. The distribution of genera in two samples. Table S3. Correlation matrix showing r values for Pearsonâ s correlation. Figure S1. Relative abundance of genes in the two hot spring samples (orange color: Yumthang hot spring, blue color: Jakrem hot spring) for selected functional KEGG pathways inferred from 16S rRNA gene data using PICRUSt.

Sustainable development aims to facilitate and support strategic changes in resource exploitation... more Sustainable development aims to facilitate and support strategic changes in resource exploitation for aligning food products with current and future consumers. Industrial sustainability achieved due to the array of emerging genomics, proteomics and metabolomics technologies. Genetic engineering techniques have been used to produce many improved enzymes and biocatalysts with high stability and specificity from their natural counterparts. The demand of novel industrial enzymes can be achieved by fishing out better enzymes by molecular screening of microbes or by improving existing enzymes by protein engineering. The use of thermophilic microorganisms as source of enzyme trends towards sustainable industrial development because of new application fields and new enzymes. Now a days the limitations of isolation techniques of thermophiles overcome by metagenomics, a new technology for discovery of genes by in silico approach and their commercial exploitation.

Proceedings of the Indian National Science Academy, 2022

Bulletin of Pure & Applied Sciences- Zoology, 2021

Comparative study on population dynamics of earthworm Amynthas alexandri carried out in two diffe... more Comparative study on population dynamics of earthworm Amynthas alexandri carried out in two different land-use systems, i.e., grassland soil at Governor's Golf field Nainital and croplands at Khurpatal and Chanfi, Nainital. Earthworms from different soil systems were collected by hand sorting and preserved in formalin for further investigations. Soil analysis revealed that the C: N ratio decreased with increasing depth in land-use systems (croplands and Grassland). The highest worm density was recorded during the rainy season in the Grassland (67.3m-2), followed by croplands (28.1 m-2). Biomass during the Rainy season was recorded higher in grassland soil (11.5 g m-2) than in the croplands (8.7 g m-2).

Matricaria chamomilla is in use for last many years as herbal remedy in various countries it is a... more Matricaria chamomilla is in use for last many years as herbal remedy in various countries it is a well known herbal plant in ancient Egypt, Greece, and Rome. The chamomile drug is included in the Pharmacopoeia of 26 countries. The present study was focused on the characterization of the main constituents of Chamomile essential oil, its antioxidant property and antimicrobial activity. The essential oil and plant extracts was extracted by Clevenger apparatus and by Soxhlet apparatus respectively with various solvents. The compound analysis of Chamomile essential oil was analysed by Gas chromatographic technique. After component analysis the antioxidant activity of methanolic extract was performed using DPPH assay. The antibacterial assays were carried out by the disc-diffusion in order to determine the antibacterial activity of oil against the strains Bacillus subtilis (ATCC 10707), Escherichia coli (ATCC 0157:H7), Proteus vulgaris (MTCC 426), and Pseudomonas aeruginosa (ATCC 27853). ...

Aim The etiology behind abnormal development of the neural tube during embryonic life is not well... more Aim The etiology behind abnormal development of the neural tube during embryonic life is not well understood. The evidence for associations of environmental cause and genes remains controversial. However recent analysis suggests excessive corresponding risks at environmental exposure levels and involvement of genes. Methods We analyzed the research on developmental defects of neural tube and developed priorities and strategies to understand its etiology and risks associated with environmental factors and genes involved. We conducted searches for peer-reviewed papers published since 1990, using the term “NTD’s”, AND “Pesticide exposure” AND “Congenital malformations”. Results Research on the investigation of neural tube defects may offer an excellent opportunity to understand and characterize high-risk population and to understand association between environmental factors and gene involved in the etiology of NTDs. Such study may lead us to evaluate etiological hypothesis i.e. the pos...

Recent Advancement in Microbial Biotechnology, 2021

Biofertilizers are the substances containing variety of microbes having the capacity to enhance p... more Biofertilizers are the substances containing variety of microbes having the capacity to enhance plant nutrient uptake by colonizing the rhizosphere and make the nutrients easily accessible to plant root hairs. Biofertilizers are well known for their cost effectiveness, environment-friendly nature, and composition. These are effective alternatives to the hazardous synthetic fertilizers. This chapter covers various types of microbial biofertilizers pronouncing symbiotic and free-living nitrogen-fixers, phosphorus-solubilizer and mobilizers, their formulations, applications of few commercially available biofertilizers toward sustainable agriculture, and recent approaches to develop next-generation biofertilizers.

Urological Science, 2019

Background: Pesticide exposure has the potential for causing acute or chronic health effects at c... more Background: Pesticide exposure has the potential for causing acute or chronic health effects at certain developmental stages of life. It can result in irreversible damage to structure and function of an organ. The exposure of females to pesticides before or after pregnancy is a matter of concern because of the possibility of having a child born with congenital disorders and developmental defects. Prenatal exposure of any toxicants can program the developing foetus to develop diseases that manifest in later life. Very few reports are available related to the pesticide exposure induced morphological changes in kidney which has prompted us to undertake this research study. Aim: The aim of this study is to evaluate the effect of pesticide, chlorpyrifos on the kidney development of rat neonates after being exposed prenatally to pesticides throughout their gestational period. Materials and Methods: Virgin female wistar rats weighing 200-250 gms were mated with adult males. It consisted of twelve, divided into two sub groups six rats per each were treated with selected pesticides, chlorpyrifos (insecticide) readily available commercially for various agricultural and non-agricultural settings. Insecticide chlorpyrifos was given at a dose of 10mg/kg per orally to the female rats throughout their gestation period. After gestation period rat neonates were sacrificed and their kidney section was processed for histopathological analysis. The SEM study of chlorpyrifos exposed kidney section of rat neonates was performed, investigating changes in morphometric and ultra-structural features of kidney in rat neonates. Results: The histopathological analysis shows morphological changes in the kidneys of prenatally pesticides exposed rat neonates markedly different from control group. Chlorpyrifos exposed neonates kidney shows damaged kidney corpuscles and glomeruli. Conclusion: This study concluded that pesticide exposure during gestation period to pregnant rats causes significant renal toxicity in their neonates.

Birth disorders defined as any abnormality in the newborn during fetal development. These disorde... more Birth disorders defined as any abnormality in the newborn during fetal development. These disorders are burden to the family and associated with substantial morbidity and mortality. In developing countries the data on the incidences of congenital disorders is very limited. The main objective of this study is to determine the incidence and profile of the congenital disorders present at birth in the newborn. This is a prospective, observational study conducted in government and private nursing home of Pithoragarh District, Uttarakhand. The information objectives of the survey were reflected in the content of the survey questionnaire included child age, gender, disorders at the time of birth. The data shows 200 cases of child birth disorders and intrauterine foetal death observed during five month period. A total of 144 cases of by birth disorders were reported and 56 cases of child death due to disorders and intrauterine foetal death were observed. 144 cases of child birth disorder in...

Microbial Diversity in the Genomic Era, 2019

Abstract Thermophilic microbes subsist in various natural and man-made niches from hot springs, u... more Abstract Thermophilic microbes subsist in various natural and man-made niches from hot springs, underwater hydrothermal vents to heated compost piles. The sequencing of 16S rRNA genes happens to the most imperative approach for microbial diversity studies. The new sequencing technologies allow sequencing of thousands of reads in a single run and the blending of high-throughput sequencing with metagenomics. Metaproteomics and single-cell genomics have enhanced the understanding of how microbial communities evolve, function, and work together. Several approaches have been proposed to mine taxonomic information/microevolution for restructuring the diversity complexity. The chapter is fixated to understand evolutionary lineages and genomic-based microbial taxonomy in the context of next-generation sequencing era.

JOURNAL OF INDIAN COLLEGE OF CARDIOLOGY, 2019

Performance is a person's achievement with regard to all tasks assigned to him. Patient satisfact... more Performance is a person's achievement with regard to all tasks assigned to him. Patient satisfaction can be influenced by factors such as, not in line with expectations and reality, service and personnel behavior is unsatisfactory, the physical condition of the environment, the cost is too high, and the promotion is not in accordance with reality. The purpose of this research is to identify The Correlation between nurse's performances and patient's satisfaction in Emergency Room of RSU GMIM Pancaran Kasih Manado. This research used cross sectional approach. The respondents are consisted of 94 patients and the sampling technique used purposive sampling. Questionnaires were used as the measuring instrument. The result of this research was analyzed by statistical analysis used chi square. The result indicates value (p=0,000) which means the Ha was accepted or there is correlation between nurse's performance and patient's satisfaction in Emergency Room of RSU GMIM Pancaran Kasih Manado. In conclusion this study shows that there are relations between the nurse performance and the patient's satisfaction in Emergency Room of GMIM Pancaran Kasih Public Hospital.

Journal of Next Generation Sequencing & Applications, 2015

Genomics Data, 2015

Jakrem hot water spring is located in the West Khasi Hill District of the state of Meghalaya, and... more Jakrem hot water spring is located in the West Khasi Hill District of the state of Meghalaya, and is one of the most popular hot springs of the state. There is a populist belief among the inhabitants and people that the hot spring water has got curative properties against various skin ailments. This is the first report on V3 hyper-variable region of 16S rDNA metagenome sequence employing Illumina platform to profile the microbial community of this less known hot spring from Meghalaya, India. Metagenome comprised of 10, 74,120 raw sequences with a sequence length of 151 bp and 56.35% G + C content. Metagenome sequence information is now available at NCBI, SRA database accession no. SRP056897. A total of 8, 77, 364 pre-processed reads were clustered into 694 OTUs (operational taxonomical units) comprising of 14 bacterial phyla including unknown phylum demonstrating 49 families. Hot spring bacterial community is dominated by Firmicutes (61.60%), Chloroflexi (21.37%), Cyanobacteria (12.96%) and unclassified bacteria (1.2%) respectively.

Journal of Mosquito Research, 2014

Mosquito faunatic studies were carried out in selected coastal villages of Odisha. Two thousand m... more Mosquito faunatic studies were carried out in selected coastal villages of Odisha. Two thousand mosquitoes belonging to 22 species under 6 genera were collected from twelve villages of Puri and Khurda Districts of Orissa. The present investigation deciphered the dominance of Culicine mosquito species (65.59%) in the coastal districts of Orissa. The study proved the dominance of Anopheles subpictus comprising 22.73% of all mosquito population studied and about 65.6% of the overall Anophelines fauna follwed by Anopheles vagus which is dominated by 10.03%. Culex. quinquefasciatus Say and the C. vishnui Theobald group are the common Culicines comprising 17.55% and 10.03% respectively. The species of Armegeres group are also equally prevalent as the Genus Culex. Shanon diversity index of the mosquito species of the two districts has been studied. Khurda District was significantly higher than the diversity in the mosquito community of Puri District of Odisha, India. The changes in mosquito fauna observed from the Orissa coastal area and Chilika Lake are due to the major ecological changes, extensive use of insecticides and development in agricultural practices, industrial development, natural calamities like severe cyclones, after effects of tsunami.