Fashola Omotola | Lagos State University, Lagos, Nigeria (original) (raw)

Papers by Fashola Omotola

The use of general descriptive names, registered names, trademarks, service marks, etc. in this p... more The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use.

Pan African Journal of Life Sciences, Mar 1, 2024

Aquatic ecology, Apr 22, 2024

Earth and environmental sciences library, 2024

Journal of Review and Research in Sciences, Jun 20, 2023

Introduction: hydrocarbon contamination of surface waters as a result of anthropogenic activity p... more Introduction: hydrocarbon contamination of surface waters as a result of anthropogenic activity poses threat to ecosystems and counter their beneficial uses. Some indigenous microbial communities have the potential to purify such waters unaided. Aims: To show the biodegradative potential of microbial communities in Lagos and Ologe Lagoons during minimal pollution with crude oil. Materials and Methods: The total heterotrophic bacteria and hydrocarbon utilising bacterial and fungal populations were estimated from Lagos and Ologe lagoon water samples contaminated with 1% crude oil over 42-day incubation period by plate count and vapour-phase transfer techniques. Residual hydrocarbons were determined by Gas chromatography. Results: The predominant bacterial genera identified from the lagoons include Enterobacter, Klebsiella and Proteus, while Escherichia, and Morganella. Aspergillus and Mucor were the predominant fungal genera in both waters. The hydrocarbon degradation rate in the Lagos Island microcosm was 65.391±0.370 mg/l/d, degradation rate constant 0.05±0.01 /d, half-life 9.559±0.093 /d and percentage degradation of 95.315 ± 0.134. Corresponding values in the Ologe water were 61.190± 8.542 mg/l/d, 8.725 ±0.389 /d, 0.055±0.003/d and 96.345±0.488 respectively. There was almost complete disappearance of the various fractions of the oil in the two samples. The microbial communities from both lagoons effectively utilised majority of the hydrocarbon fractions after 42 days where 66.890±1.075 and 100±000 were recorded for benzene, toluene had 100±000 percent degradation, anthracene 96.755±0.119 and 99.726±0.026, and pristane had 91.674±0.222 and 99.943±0.015 while phytane had 96.44±0.058 and 99.670±0.104 respectively. Conclusion: Efficient biodegradation of moderate contamination crude oil could be achieved by indigenous microbial flora present in Lagos and Ologe lagoon waters.

Funding information The authors received no funding. Abstract: Introduction: Indiscriminate dumpi... more Funding information The authors received no funding. Abstract: Introduction: Indiscriminate dumping of spent oils enriched with heavy metals has led to increase in heavy metals load in the soil. Heavy metals exert toxic effects on biodegradation of organic pollutant in cocontaminated soil and there is need to find suitable strategies for their removal. Aim: The aim of this study was to assess the heavy metals resistance capability of indigenous Bacillus species in hydrocarbon polluted soil to nickel (Ni), Cadmium (Cd), Lead (Pb) and Chromium (Cr). Materials and Methods: Heavy metal tolerant bacteria were isolated from hydrocarbon polluted soil using Luria-Berthani agar supplemented with the respective metals and spread plate techniques. The isolates were putatively identified on the basis of their colonial morphology and biochemical characteristics and their antibiotics susceptibility pattern were evaluated using disc diffusion method. Results: The maximum tolerable concentration (M...

Authorea (Authorea), Jun 16, 2023

Increased urbanization and technological advancement in recent years has resulted in increased di... more Increased urbanization and technological advancement in recent years has resulted in increased discharge of heavy metals in the environment which has become a major menace all over the world. Due to their inert nature, metals can build up in the environment and have a negative impact on the living biota present. Bacteria play a crucial role in determining the environmental fate of metals by altering their structure and dispersal. Their contribution to the transformation and elimination of toxic metals is crucial. Despite the various roles played by bacteria in processing and detoxifying heavy metals in the environment, they are also vulnerable to metal toxicity. Heavy metals can cause adverse effects on bacterial cells depending on their bio-availability and the absorbed dose. This review discussed some of the various ways that heavy metals exert their toxic effects on bacteria cells and the likely factors that can aid their toxic effects to bacteria cells.

Natural and Engineering Sciences, 2018

Bacteriocins are proteinaceous toxins produced by bacteria to inhibit the growth of similar or cl... more Bacteriocins are proteinaceous toxins produced by bacteria to inhibit the growth of similar or closely related bacterial strains. Fermented Parkia biglobosa seeds (African locust bean) were screened for bacteriocin-producing lactic acid bacteria (LAB) with the characterization of putative bacteriocins. Bacteriocin-producing lactic acid bacteria (LAB) were identified by 16s rDNA sequencing. Molecular sizes of the bacteriocins were determined using the tricine-sodium dodecyl sulphate-polyacrylamide gel electrophoresis (tricine-SDS–PAGE) and effects of enzymes, pH, detergents and temperature on bacteriocin activity investigated, using standard procedures. Bacteriocins production and activities were measured by spectrophotometric analysis. Statistical analysis was carried out using student t-test and Analyses of Variance. Bacteriocigenic LAB isolated were Lactobacillus plantarum Z1116, Enterococcus faecium AU02 and Leuconostoc lactis PKT0003. They inhibited the growth of both Gram-posit...

Food Security and Safety, 2021

Bacteriocins This study aimed to investigate the antibacterial activity of lactic acid bacteria (... more Bacteriocins This study aimed to investigate the antibacterial activity of lactic acid bacteria (LAB) isolated from fresh pepper and tomatoes against common food borne pathogens. Man Rogosa Sharpe (MRS) broth and agar were used for the isolation of LAB from the food products and whose antagonistic properties were tested against Bacillus cereus, Staphylococcus aureus, Streptococcus sp., E. coli, Klebsiella pneumoniae and Pseudomonas aeruginosa using well diffusion assay method. Four LAB species were isolated and these included Streptococcus pyogenes, Enterococcus faecalis, Lactococcus casei and Lactococcus fermentii. The percentage occurrence of LAB species ranged from 11.77% to 35.29%. E. faecalis exerted the strongest antibacterial activity against all selected pathogenic bacteria while L. casei showed the weakest activity. It was concluded that the isolated LAB showed remarkable inhibitory effect against tested pathogenic strains. It is therefore suggested that these potent isolat...

Introduction: Indiscriminate dumping of spent oils enriched with heavy metals has led to increase... more Introduction: Indiscriminate dumping of spent oils enriched with heavy metals has led to increase in heavy metals load in the soil. Heavy metals exert toxic effects on biodegradation of organic pollutant in cocontaminated soil and there is need to find suitable strategies for their removal. Aim: The aim of this study was to assess the heavy metals resistance capability of indigenous Bacillus species in hydrocarbon polluted soil to nickel (Ni), Cadmium (Cd), Lead (Pb) and Chromium (Cr). Materials and Methods: Heavy metal tolerant bacteria were isolated from hydrocarbon polluted soil using Luria-Berthani agar supplemented with the respective metals and spread plate techniques. The isolates were putatively identified on the basis of their colonial morphology and biochemical characteristics and their antibiotics susceptibility pattern were evaluated using disc diffusion method. Results: The maximum tolerable concentration (MTC) of the four heavy metals to the selected isolates was 2 mM....

Malaysian Journal of Microbiology, 2020

Aims: Water is described as safe and wholesome when it is free from pathogenic microorganisms and... more Aims: Water is described as safe and wholesome when it is free from pathogenic microorganisms and chemical substances that are hazardous to human health. This study aimed to investigate the microbial quality of water used for drinking, cooking, bathing and other purposes at universities in Nigeria. Methodology and results: Water samples were collected from forty-four storage tanks across four selected universities. Total viable bacteria in the water samples were cultivated using the plate count agar. The isolation of total coliform and Escherichia coli were carried out on HarlequinTM E. coli/coliform agar (HA) medium, while media-faecal coliform was used for faecal coliform employing the membrane filtration technique. Physicochemical parameters such as alkalinity, pH, total alkalinity, total dissolved solid, total suspended solid, electrical conductivity, total hardness, fluoride and chloride ion concentrations, were evaluated in accordance with standard procedures. Data were compar...

Journal of Human Ecology, 2016

This study was carried out to identify the common bacterial pathogens associated with urinary tra... more This study was carried out to identify the common bacterial pathogens associated with urinary tract infections and their susceptibility patterns to common antimicrobial agents. One hundred (100) clean catch midstream urine samples from patients attending three different hospitals in Lagos, Nigeria were analyzed. A standard microbiologic procedure was used to culture the urine and the identification was done using appropriate biochemical tests. Antibiotic sensitivity of the isolates ones was determined using the disk diffusion technique on the Mueller-Hinton agar. Out of the one hundred (100) samples analyzed, 74 yielded significant bacteriuria and out of these, 83.79 percent were Gram negative bacteria. The most prevalent isolate identified were Klebsiella pneumonia (40.54%), followed by Escherichia coli (35.14%), Staphylococcus aureus (16.22%), Enterobacter spp (5.40%) and Proteus (2.70%). The bacterial isolates were more sensitive to Nitrofurantoin with percentage sensitivity of 7...

Canadian Journal of Soil Science, 2020

Mine tailings are a potential source of heavy metals (HM) that can be toxic to microbes, plants, ... more Mine tailings are a potential source of heavy metals (HM) that can be toxic to microbes, plants, and animals in aquatic and terrestrial ecosystems. Bacteria have evolved several mechanisms to tolerate the uptake of HM ions. This study aimed to assess the physicochemical properties, concentrations of selected HM and metalloids [arsenic (As), nickel (Ni), lead (Pb), zinc (Zn), cadmium (Cd), and cobalt (Co)], and isolate potential metal-tolerant bacteria present at three abandoned gold mining sites with a view of understanding how tailings characteristics vary and the implications on microbial activities in tailings dumps. Heavy-metal-tolerant bacteria were isolated from the samples using minimum inhibitory and maximum tolerable concentrations of the Ni, Pb, Zn, Cd, and Co. The substrates of the studied sites were acidic and deficient in nutrients. High metals and metalloid concentrations in the order Zn > Ni > Co > As > Pb > Cd were recorded in some of the studied sites...

International Journal of Environmental Research, 2019

Chemical precipitation, oxidation/reduction, filtration, ion-exchange, reverse osmosis, membrane ... more Chemical precipitation, oxidation/reduction, filtration, ion-exchange, reverse osmosis, membrane technology, evaporation and electrochemical treatment as remediation technologies have various shortcomings which have fueled the search for more environmentally friendly and cost-effective methods of remediating heavy metal contaminated environments. Indigenous bacteria in heavy metal contaminated sites present a possible solution to this concern. This study assessed the potential of indigenous heavy metal resistant bacteria as immobilization agents of Pb, Ni and Zn in Au mine tailings. Tailings from three abandoned Au mining environments; mine tailings A (MA), mine tailings B (MB), and Tudor shaft (TS) were collected and indigenous heavy metal resistant bacteria present in the tailings isolated. The isolated bacteria OMF 532 (E. asburiae) and OMF 003 (B. cereus) were used in bioaugmenting Ni-, Pb-and Zn-spiked tailings to determine the potential of the isolates to immobilize these metals. The immobilization potential of the isolates as determined by the difference in metal mobility in the tailings samples before and after bioaugmentation was used to assess the immobilization potential of the bacterial isolates. Mobility factor (MF) of Ni in the samples was reduced from 16.4 to 6.2, and 17.6 to 7.4 in MB and MA, respectively, reflecting a 35% decrease in Ni mobility. Lead and Zn mobility in the samples also showed a decrease of 90% and 60%, respectively, after bioaugmentation. Though MF values for Ni, Pb and Zn in the TS samples indicated low level of mobility of these elements at the site, bioaugmentation further reduced their mobility by 25-35% for Ni, 95% for Pb, and 10-30% for Zn. The results of this study show that indigenous bacteria in the tailings have the potential to reduce the bioavailable fractions of the three metals studied in the mine tailing and could be further exploited in heavy metal remediation of the sites. Article Highlights The study investigated the potential of indigenous bacteria to immobilize selected heavy metals in tailings samples. The highlights of the manuscript include the following: • The study identified Bacillus cereus OMF 003 and Enterobacter asburiae OMF 532 as heavy metal resistant bacteria in Au mine tailings. • Bioaugmenting tailings with the bacterial isolates reduced the mobility Factor of Ni in the samples by up to 35% for Ni, 90% for Pb and 60% for Zn. • Indigenous Bacillus cereus OMF 003 and Enterobacter asburiae OMF 532 presents significant opportunities for heavy metal immobilization in tailings contaminated environments.

Journal of Life Sciences, 2015

Constituting the most important group of microorganisms that offered to human consumption through... more Constituting the most important group of microorganisms that offered to human consumption through food, yeasts are a substantial part of microflora in dairy products. Due to sophisticated enzyme production especially high proteolytic and lipolytic activities, some of the yeast species play an essential role in many processes such as fermentation, ripening, production of aroma precursors and deterioration in dairy product indicating these products as a source of extracellular enzyme producer yeast strains. In this study, butter samples were collected from delicatessens and grocery stores in different locations in İzmir province in order to isolate enzyme producers. A total of 40 yeast strains were isolated and investigated for their lipase, protease and amylase activities on solid medium consisting of tributyrin, skim milk and starch, respectively. Sixteen yeast isolates were found to be positive for enzyme production. Lipase and protease activities were observed in 11 yeast strains (68,75%), while only one yeast strain (6,25%) was found to be positive for protease and amylase production. 4 of yeast strains (25%) were able to produce only lipase enzyme. Molecular characterization of the yeast strains based on 26S rRNA region D1/D2 domains revealed that the isolates belonged to Candida zeylanoides (62,5%) and Pichia fermentas (37,5%) species.

International Journal of Environmental Research and Public Health, 2016

Mining activities can lead to the generation of large quantities of heavy metal laden wastes whic... more Mining activities can lead to the generation of large quantities of heavy metal laden wastes which are released in an uncontrolled manner, causing widespread contamination of the ecosystem. Though some heavy metals classified as essential are important for normal life physiological processes, higher concentrations above stipulated levels have deleterious effects on human health and biota. Bacteria able to withstand high concentrations of these heavy metals are found in the environment as a result of various inherent biochemical, physiological, and/or genetic mechanisms. These mechanisms can serve as potential tools for bioremediation of heavy metal polluted sites. This review focuses on the effects of heavy metal wastes generated from gold mining activities on the environment and the various mechanisms used by bacteria to counteract the effect of these heavy metals in their immediate environment.