Olusola Ojo-omoniyi | Lagos State University, Lagos, Nigeria (original) (raw)

Papers by Olusola Ojo-omoniyi

World Journal of Microbiology and Biotechnology

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International Journal of Current Microbiology and Applied Sciences, Jun 10, 2022

InTech eBooks, Jun 14, 2013

Tropical soil samples were collected from three different locations Southwest, Nigeria. The first... more Tropical soil samples were collected from three different locations Southwest, Nigeria. The first location was Lagos State University: Faculty of Science, Faculty of Management Science and Lagos State University second gate. The second location was Covenant University: soil was collected from the College of Science and Technology, College of Developmental Studies, Lecture theatre and the Covenant University farm. The third location: soil samples were collected from Shagamu. All soil samples were collected at a depth of 0 cm to 30 cm using a hand trowel and were transported to the laboratory in sterile polythene bags. Control soil samples were obtained from Badagry beach in Lagos. The purpose of this study is to isolate native Rhizobium sp. from tropical soils southwest Nigeria with the ability to nodulate the legume Glycine max (Soya beans). Serial dilution of the soil samples following aseptic processes generated dilution factors 10-4 and 10-5 used as inocula for the pour plate method using Nutrient Agar (NA) and Yeast Extract Mannitol Agar (YEMA) as growth media. The plates were incubated at 28 ± 2°C for 48 h which made visible growth to be observed. Visible differences between the numbers of colonies of dilution factors 10-4 and 10-5 were observed on both NA and YEMA after the colonies were counted. Physical examination of the culture plates showed that all of the colonies appeared round, mucoid and 58.3% of the colonies were creamy white on YEMA. Microscopic examination revealed motile Gram-negative rods on YEMA while 50% of the isolates on NA were non-motile rods. The mean Rhizobium sp. population detected in the soil samples was 0.33 × 10-5 CFU/g. Conclusively, the presence of indigenous rhizobia population specific for Soya bean in the soils evident by nodulation of the legume 8 Week after Planting (WAP) confirms both the symbiotic relationship and its relevance to sustainable agricultural practice.

Background Microorganisms perform their metabolic processes rapidly with remarkable specificity u... more Background Microorganisms perform their metabolic processes rapidly with remarkable specificity under ambient conditions catalyzed by their diverse enzymesmediated reactions. The introduction of microbial enzymes as an alternative to harsh chemical technologies has led to intensive exploration of natural microbial biodiversity to discover microbial enzymes with possible application in waste recycling under appropriate conditions (Gautam et al., 2010). Cellulase is the key enzyme for the conversion of cellulosic materials into simple sugars which can serve as feed-stock for the production of different chemicals and fuels via anaerobic fermentation (Ryu and Mandels, 1980). Cellulolytic enzymes play an important role in natural biodegradation processes in which plant ligno-cellulosic materials are effectively degraded by cellulolytic fungi, bacteria, Actinomycetes and protozoa. Many fungi capable of degrading cellulose synthesize large quantities of extracellular cellulases that are more efficient in depolymerising the cellulose substrate. Objectives This research was to carry out an environmental surveillance for cellulose-degrading microorganisms present in wastes dumpsites in Lagos, southwest Nigeria. Methods Soil and leachate samples were collected from three dumpsites in Lagos following aseptic procedures and the microbial species were isolated using Nutrient agar and Potato-dextrose agar media respectively. The cellulase-producing microbial species were characterized following conventional and standard methods. They were then screened for cellulase activity using the Cellulose Congo-Red plate technique. The diameters of clear zone of inhibition were measured in millimeters (mm) (Lu et al., 2005; Guatam et al., 2012). Result All isolates were cellulase producers, with Bacillus licheniformis having the highest cellulase activity and hydrolytic value (34mm and 8.5 respectively) among the bacterial species while Aspergillus sp. had the highest cellulase activity (63mm) and hydrolytic value (15.8) among the fungal species. The colonial and microscopic characterization of the fungal isolates showed that they belong to the genera; Penicillium, Aspergillus and Trichoderma. Conclusion This result suggested that these microorganisms utilized the available sources of cellulose present in wastes both for growth and biodegradative processes. Consequently, this will enhance the sustainable solid waste management practice for megacities as well as the public health in developing economies.

IOP conference series, Dec 6, 2018

Wastewater samples, industrial effluents from food industry and cow dung were obtained asepticall... more Wastewater samples, industrial effluents from food industry and cow dung were obtained aseptically and the physico-chemical properties were determined following standard procedures. Serial dilution technique was used in isolating native pyrazine-degraders from composite wastewater and cow dung samples obtained from Agbara and Apapa wastewater treatment plant (WTP) as well as Agege and Agbara Abattoirs respectively. Nutrient agar and Mineral salt medium supplemented with (3%) Pyrazine-2-carboxamide were used for enrichment for the isolates. Standard and conventional methods were used for the characterization and identification of pyrazine degraders. Gas chromatography (GC) flame ionization detector (FID) was used to determine the degree of mineralization of pyrazine. The mean pyrazine-degrader count from wastewater samples was 1.1×10 6 cfu/ml while that of cow dung was 9.7×10 5 cfu/ml. The GC peaks revealed that the initial quantity of pyrazine (15mg/l) was mineralized leaving behind (0.175mg/l) residues after 21 days of its introduction. The bacterial genera found utilizing pyrazine as source of C and N energy includes; Bacillus sp.,Flavobacterium sp.,Corynebacterium sp., Pseudomonas sp.,Streptococcus sp. and Staphylococcus sp. The biodegradability of pyrazine by tropical indigenous bacterial consortium was established and the convincing evidence of its mineralization was with gas chromatography. The objectives of the current study was to isolate and identify indigenous pyrazine-degraders from tropical wastewater and cow dung.

Food and Environment Safety, Jul 30, 2022

International Journal of Current Microbiology and Applied Sciences

Digital image processing involves the usage of a functional algorithm to process images with spec... more Digital image processing involves the usage of a functional algorithm to process images with special regions of interest. In most case scenarios, this is termed as an active aspect of digital signal processing; image processing comes with several rewards over analog image processing. Its relevance and application spans Autonomous Vehicles, Biometric fingerprint technologies as well as Face recognition applications. Reliable statistics through feature engineering from the image can be extracted and in turn serve as focus points of deep learning insights. Besides, its application in monitoring Climatic changes, Agricultural crop yields, security measures, industrial manufacturing as well as medical fields exponentially advances each day. Meanwhile, deep learning being a feature of Artificial Intelligence has brought forward several useful models that is being used as transfer base for further model accuracies and baselines. In this study, we make use of a certain Microscopy datasets, ...

IOP Conference Series: Earth and Environmental Science, 2018

International Journal of Research, Jun 30, 2021

Ice cream is a nutritionally enriched dairy product consumed by all age groups. This study evalua... more Ice cream is a nutritionally enriched dairy product consumed by all age groups. This study evaluated the microbial quality of some brands of retail ice cream products randomly purchased from vendors in Lagos metropolis by isolation, identification and characterization of the microbial isolates.A total of six different brands of ice cream products were collected and brought into the laboratory and kept in the refrigerator at 4oC prior to analysis. The microbiological quality of some brands of ice cream products obtained from vendors in Lagos metropolis was determined following Standard and conventional methods for the identification and characterization of microbial isolates. Five bacterial species were prominent: Staphylococcus aureus, Klebsiella species, Bacillus species, Escherichia coli and Enterobacter species. Bacterial growth were observed for all samples ranging from 1.14 x 103 to 6.0 x 104 (cfu/ml). Staphylococcus aureus recorded highest percentage occurrence of 100%, follow...

African Journal of Infectious Diseases

Biodegradation of atrazine, a xenobiotic-substance recently introduced into agricultural practice... more Biodegradation of atrazine, a xenobiotic-substance recently introduced into agricultural practice in Nigeria was assessed using native soil microorganisms. Field experiment and laboratory studies confirmed that microbial consortium utilize the hydrocarbon component of atrazine and thereby mineralize the somewhat recalcitrant organic chemical. The atrazine-degraders were isolated on Minimal salt medium and Sabouraud dextrose agar supplemented with atrazine at 0.05g/L and incubated at 28 ±2 o C for 72 hrs and 5 days respectively. The sampled agricultural soil was of alkaline pH while the optimum pH at which the microbial consortium metabolizes atrazine was acidic (mean pH 5.63). Total viable count of bacterial atrazine-degraders was 602.5 x 10 5 cfu/ml. Bacterial atrazine-degraders were: Micrococcus sp., Bacillus sp. Enterobacter Sp and Pseudomonas sp. while fungal atrazine-degraders were: Aspergillus fumigatus, A. niger, Penincillium sp and Fusarium sp.