Pradeep Rp | Visveswaraya Technological University (original) (raw)
Address: Bangalore, Karnataka, India
less
Uploads
Papers by Pradeep Rp
Tetrahedron Letters, 2005
A synthesis of labeled oligonucleotides incorporating a new chemically cleavable linker (III) via... more A synthesis of labeled oligonucleotides incorporating a new chemically cleavable linker (III) via a two-step method is described. The labeled oligomers obtained after cleavage and deprotection reactions [treatment with anhydrous tert-butylamine and dry methanol, 1:1 (v/v) for 12 h at room temperature, and lyophilization followed by subsequent reaction with aq NH 4 OH and methylamine (40%), 1:1 (v/v) for 5 min at 65°C] were analyzed by RP-HPLC. A distinctive feature of this protocol is that free oligomers can be recovered from their labeled analogs under mild conditions (0.2 M NaOH containing 0.5 M NaCl over 30 min at room temperature) and are comparable to the corresponding standard oligonucleotides (HPLC).
Tetrahedron Letters, 2005
A synthesis of labeled oligonucleotides incorporating a new chemically cleavable linker (III) via... more A synthesis of labeled oligonucleotides incorporating a new chemically cleavable linker (III) via a two-step method is described. The labeled oligomers obtained after cleavage and deprotection reactions [treatment with anhydrous tert-butylamine and dry methanol, 1:1 (v/v) for 12 h at room temperature, and lyophilization followed by subsequent reaction with aq NH 4 OH and methylamine (40%), 1:1 (v/v) for 5 min at 65°C] were analyzed by RP-HPLC. A distinctive feature of this protocol is that free oligomers can be recovered from their labeled analogs under mild conditions (0.2 M NaOH containing 0.5 M NaCl over 30 min at room temperature) and are comparable to the corresponding standard oligonucleotides (HPLC).