Astrocytes as Antigen Presenting Cells for Primary and Secondary T Cell Responses: Effect of Astrocyte Infection by Murine Hepatitis Virus (original) (raw)

Abstract

CD4+ T cell lines specific for murine hepatitis virus (MHV)-JHM or myelin basic protein (MBP) proliferated when cultured together with MHC class I and II positive syngeneic rat astrocytes and either inactivated virus or MBP as antigen. The magnitude of the T cell proliferative response was comparable to that seen when thymocytes were used as a source of antigen presenting cells (APC).

In contrast, MHC class I and II positive astrocytes were unable to significantly stimulate the proliferation of highly purified populations of naive CD4+ and CD8+ T cells in an allogeneic mixed lymphocyte reaction (MLR). Both T cell populations proliferated when mixed with allogeneic lymph node cells. Infection of the astrocytes with a variant of MHV-JHM (PI-AS22D) did not alter this cells incapacity to stimulate the naive CD4+ and CD8+ T cells to proliferate.

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Authors and Affiliations

  1. Institute for Virology and Immunobiology, University of Würzburg, Würzburg, West Germany
    Rainald Mößner, Jonathon Sedgwick, Egbert Flory, Heiner Körner, Helmut Wege & Volker ter Meulen

Authors

  1. Rainald Mößner
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  2. Jonathon Sedgwick
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  3. Egbert Flory
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  4. Heiner Körner
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  5. Helmut Wege
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  6. Volker ter Meulen
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Editors and Affiliations

  1. AFRC Institute for Animal Health, Huntingdon, UK
    David Cavanagh Ph.D.
  2. Department of Pathology, University of Cambridge, UK
    T. David K. Brown Ph.D.

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© 1990 Plenum Press, New York

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Mößner, R., Sedgwick, J., Flory, E., Körner, H., Wege, H., ter Meulen, V. (1990). Astrocytes as Antigen Presenting Cells for Primary and Secondary T Cell Responses: Effect of Astrocyte Infection by Murine Hepatitis Virus. In: Cavanagh, D., Brown, T.D.K. (eds) Coronaviruses and their Diseases. Advances in Experimental Medicine and Biology, vol 276. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5823-7\_88

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