Intronic Elements Appear Essential for the Differentiation-Specific Expression of Acetylcholinesterase in C2C12 Myotubes (original) (raw)

Abstract

Muscle development involves a complex series of events. Mesodermal progenitor cells commit to become myoblasts, myoblasts fuse and, accompanied by cell cycle arrest, differentiate to myotubes. As a cell culture paradigm to a portion of the differentiation process we have chosen to study murine C2C12 cells. C2C12 myoblasts remain undifferentiated and proliferative when grown in the presence of serum due to cell cycle regulated gene products (1). Myoblasts produce virtually no acetylcholinesterase (AChE). Withdrawal of serum stops cell division and induces fusion of myoblasts into multinucleate myotubes which produce both cell associated and exported AChE.

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Authors and Affiliations

  1. Department of Pharmacology 0636, University of California at San Diego, La Jolla, California, 92093, USA
    Shelley Camp & Palmer Taylor

Authors

  1. Shelley Camp
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  2. Palmer Taylor
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Editor information

Editors and Affiliations

  1. Walter Reed Army Institute of Research, Washington, D.C., USA
    Bhupendra P. Doctor & Mary K. Gentry &
  2. University of California, San Diego La Jolla, California, USA
    Palmer Taylor
  3. University of Iowa, Iowa City, Iowa, USA
    Daniel M. Quinn
  4. University of Miami School of Medicine, Miami, Florida, USA
    Richard L. Rotundo

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© 1998 Springer Science+Business Media New York

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Camp, S., Taylor, P. (1998). Intronic Elements Appear Essential for the Differentiation-Specific Expression of Acetylcholinesterase in C2C12 Myotubes. In: Doctor, B.P., Taylor, P., Quinn, D.M., Rotundo, R.L., Gentry, M.K. (eds) Structure and Function of Cholinesterases and Related Proteins. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1540-5\_7

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