Phagocytosis of Candida albicans by RNAi-Treated Drosophila S2 Cells (original) (raw)

Abstract

Phagocytosis is a highly conserved aspect of innate immunity. Drosophila melanogaster has an innate immune system with many similarities to that of mammals and has been used to successfully model many aspects of innate immunity. The recent availability of Ribo Nucleic Acid interference (RNAi) libraries for Drosophila has made it possible to efficiently screen for genes important in aspects of innate immunity. We have screened an RNAi library representing 7216 fly genes conserved among metazoans to identify proteins required for the phagocytosis of the human fungal pathogen Candida albicans.

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Author information

Authors and Affiliations

  1. Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, California, USA
    Shannon L. Stroschein-Stevenson
  2. Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, Canada
    Edan Foley
  3. Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, USA
    Patrick H. O’Farrell
  4. Department of Microbiology and Immunology, and Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, USA
    Alexander D. Johnson

Authors

  1. Shannon L. Stroschein-Stevenson
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  2. Edan Foley
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  3. Patrick H. O’Farrell
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  4. Alexander D. Johnson
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Editor information

Editors and Affiliations

  1. Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstrasse 12, 70569 Stuttgart, Germany
    Steffen Rupp & Kai Sohn &

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Stroschein-Stevenson, S.L., Foley, E., O’Farrell, P.H., Johnson, A.D. (2009). Phagocytosis of Candida albicans by RNAi-Treated Drosophila S2 Cells. In: Rupp, S., Sohn, K. (eds) Host-Pathogen Interactions. Methods in Molecular Biology, vol 470. Humana Press. https://doi.org/10.1007/978-1-59745-204-5\_24

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