3D-FISH on Cultured Cells Combined with Immunostaining (original) (raw)

References

  1. Bolzer, A., Kreth, G., Solovei, I., Koehler, D., Saracoglu, K., Fauth, C., Muller, S., Eils, R., Cremer, C., Speicher, M. R., and Cremer, T. (2005) Three-dimensional maps of all chromosomes in human male fibroblast nuclei and prometaphase rosettes. PLoS Biol 3, e157.
    Article PubMed Google Scholar
  2. Brown, J. M., Green, J., das Neves, R. P., Wallace, H. A., Smith, A. J., Hughes, J., Gray, N., Taylor, S., Wood, W. G., Higgs, D. R., Iborra, F. J., and Buckle, V. J. (2008) Association between active genes occurs at nuclear speckles and is modulated by chromatin environment. J Cell Biol 182, 1083–1097.
    Article PubMed CAS Google Scholar
  3. Finlan, L. E., Sproul, D., Thomson, I., Boyle, S., Kerr, E., Perry, P., Ylstra, B., Chubb, J. R., and Bickmore, W. A. (2008) Recruitment to the nuclear periphery can alter expression of genes in human cells. PLoS Genet 4, e1000039.
    Article PubMed Google Scholar
  4. Goetze, S., Mateos-Langerak, J., Gierman, H. J., de Leeuw, W., Giromus, O., Indemans, M. H., Koster, J., Ondrej, V., Versteeg, R., and van Driel, R. (2007) The three-dimensional structure of human interphase chromosomes is related to the transcriptome map. Mol Cell Biol 27, 4475–4487.
    Article PubMed CAS Google Scholar
  5. Kupper, K., Kolbl, A., Biener, D., Dittrich, S., von Hase, J., Thormeyer, T., Fiegler, H., Carter, N. P., Speicher, M. R., Cremer, T., and Cremer, M. (2007) Radial chromatin positioning is shaped by local gene density, not by gene expression. Chromosoma 116, 285–306.
    Article PubMed Google Scholar
  6. Meaburn, K. J., and Misteli, T. (2008) Locus-specific and activity-independent gene repositioning during early tumorigenesis. J Cell Biol 180, 39–50.
    Article PubMed CAS Google Scholar
  7. Meaburn, K. J., Newbold, R. F., and Bridger, J. M. (2008) Positioning of human chromosomes in murine cell hybrids according to synteny. Chromosoma 117, 579–591.
    Article PubMed Google Scholar
  8. Osborne, C. S., Chakalova, L., Mitchell, J. A., Horton, A., Wood, A. L., Bolland, D. J., Corcoran, A. E., and Fraser, P. (2007) Myc dynamically and preferentially relocates to a transcription factory occupied by Igh. PLoS Biol 5, e192.
    Article PubMed Google Scholar
  9. Takizawa, T., Gudla, P. R., Guo, L., Lockett, S., and Misteli, T. (2008) Allele-specific nuclear positioning of the monoallelically expressed astrocyte marker GFAP. Genes Dev 22, 489–498.
    Article PubMed CAS Google Scholar
  10. Teller, K., Solovei, I., Buiting, K., Horsthemke, B., and Cremer, T. (2007) Maintenance of imprinting and nuclear architecture in cycling cells. Proc Natl Acad Sci U S A 104, 14970–14975.
    Article PubMed CAS Google Scholar
  11. Bridger, J. M., and Lichter, P. (1999) Analysis of mammalian interphase chromosomes by FISH and immunofluorescence, in Chromosome structural analysis: A practical approach (Bickmore, W. A., Ed.) pp 103–121, Oxford University Press, Oxford.
    Google Scholar
  12. Ersfeld, K., and Stone, E. M. (2000) Simultaneous in situ detection of DNA and proteins, in Protein localization by fluorescence microscopy (Allan, V. J., Ed.), Oxford University Press, Oxford.
    Google Scholar
  13. Solovei, I., Walter, J., Cremer, M., Habermann, H., Schermelleh, L., and Cremer, T. (2002) FISH on three-dimensionally preserved nuclei, in FISH (Beatty B., Mai, S., Squire, J., Eds.) pp 119–157, Oxford University Press, Oxford.
    Google Scholar

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