A genomic library of Lycopersicon pennellii in L. esculentum: A tool for fine mapping of genes (original) (raw)
Abstract
The cultivated tomato contains only a small fraction of the genetic variation present in its wild relatives. In order to use the wild germplasm in tomato breeding and genetic studies we developed a new kind of genetic resource which is composed of 50 L. esculentum lines each containing a single introgression from the green fruited species L. pennellii (LA 716). Each of the introgression lines is nearly isogenic to the cultivated tomato; these lines provide complete coverage of the wild species genome. The lines contain on the average an introgression of 33 cM from a total genome size of 1200 cM. The size and identity of the introgressed segments was determined based on RFLP analysis of 350 markers. This resource can be viewed as a genomic library of the wild species in the cultivated background. It covers the entire genome with single independent ‘inserts’ per line and therefore every phenotypic difference between the introgression lines can be associated with the unique introgressed segment. The development and potential application of this resource are discussed.
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Authors and Affiliations
- Faculty of Agriculture, Department of Field and Vegetable Crops and the Otto Warburg Center for Biotechnology, The Hebrew University of Jerusalem, P.O. Box 12, 76100, Rehovot, Israel
Yuval Eshed & Dani Zamir
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- Yuval Eshed
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Eshed, Y., Zamir, D. A genomic library of Lycopersicon pennellii in L. esculentum: A tool for fine mapping of genes.Euphytica 79, 175–179 (1994). https://doi.org/10.1007/BF00022516
- Issue Date: January 1994
- DOI: https://doi.org/10.1007/BF00022516