New plant binary vectors with selectable markers located proximal to the left T-DNA border (original) (raw)

Abstract

Five new binary vectors have been constructed which have the following features: (1) different plant selectable markers including neomycin phosphotransferase (nptII), hygromycin phosphotransferase (hpt), dihydrofolate reductase (dhfr), phosphinothricin acetyl transferase (bar), and bleomycin resistance (ble); (2) selectable markers are located near the T-DNA left border and; (3) selectable marker and β-glucuronidase (uidA) reporter genes are divergently organized for efficient expression, and can easily be removed or replaced as needed.

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References

  1. Becker D: Binary vectors which allow the exchange of plant selectable markers and reporter genes. Nucl Acids Res 18: 203 (1990).
    PubMed Google Scholar
  2. Bevan M: Binary Agrobacterium vectors for plant transformation. Nucl Acids Res 12: 8711–8721 (1984).
    PubMed Google Scholar
  3. DeBlock M, Botterman J, Vandewiele M, Dockx J, Thoen C, Gossele V, Movva NR, Thompson C, VanMontagu M, Leemans J: Engineering herbicide resistance in plants by expression of a detoxifying enzyme. EMBO J 6: 2513–2518 (1987).
    Google Scholar
  4. Hille J, Verheggen F, Roelvink P, Franssen H, Kammen AV, Zabel P: Bleomycin resistance: a new dominant selectable marker for plant cell transformation. Plant Mol Biol 7: 171–176 (1986).
    Google Scholar
  5. Jefferson RA, Kavanagh TA, Bevan MW: GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901–3907 (1987).
    PubMed Google Scholar
  6. Kemper E, Grevelding C, Schell J, Masterson R: Improved method for the transformation of Arabidopsis thaliana with chimeric dihydrofolate reductase constructs which confer methotrexate resistance. Plant Cell Rep 11: 118–121 (1992).
    Article Google Scholar
  7. Töpfer R, Gronenborn B, Schell J, Steinbiss H-H: Uptake and transient expression of chimeric genes in seedderived embryos. Plant Cell 1: 133–139 (1980).
    Google Scholar
  8. White J, Chang S-YP, Bibb MJ, Bibb MJ: A cassette containing the bar gene of S. hygroscopicus: a selectable marker for plant transformation. Nucl Acids Res 18: 1062 (1990).
    PubMed Google Scholar
  9. Yenofsky RL, Fine M, Pellows JW: A mutant neomycin phosphotransferase II gene reduces the resistance of transformants to antibiotic selection pressure. Proc Natl Acad Sci USA 87: 3435–3439 (1990).
    PubMed Google Scholar
  10. Zambryski P: Basic processes underlying _Agrobacterium_-mediated DNA transfer to plant cells. Annu Rev Genet 22: 1–30 (1988).
    Article PubMed Google Scholar

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Authors and Affiliations

  1. Max-Planck-Institut für Züchtungsforschung, Carl von Linné Weg 10, D-5000, Köln 30, Germany
    Detlef Becker, Elke Kemper, Jeff Schell & Robert Masterson

Authors

  1. Detlef Becker
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  2. Elke Kemper
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  3. Jeff Schell
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  4. Robert Masterson
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Becker, D., Kemper, E., Schell, J. et al. New plant binary vectors with selectable markers located proximal to the left T-DNA border.Plant Mol Biol 20, 1195–1197 (1992). https://doi.org/10.1007/BF00028908

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