Preliminary formulation of a chemically defined medium for insect cell cultures (original) (raw)

Abstract

A chemically defined medium for insect cell cultures was devised. The medium is protein-free. It consisted of 105 chemicals. In this medium, a continuous cell line from flesh fly, Sarcophaga peregrina (NIH-SaPe-4) could be passed more than 200 times. The medium is useful for studies on nutritional requirements by insect cells as well as for biochemical studies on insect cell cultures.

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Authors and Affiliations

1. Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan
   Jun Mitsuhashi

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Mitsuhashi, J. Preliminary formulation of a chemically defined medium for insect cell cultures.Methods Cell Sci 18, 293–298 (1996). https://doi.org/10.1007/BF00127906

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• Accepted: 25 September 1996
• Issue date: December 1996
• DOI: https://doi.org/10.1007/BF00127906

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