Metabolic burden as reflected by maintenance coefficient of recombinant Escherichia coli overexpressing target gene (original) (raw)
Abstract
Metabolic burden as a consequence of overexpression of target gene in a recombinant strain of E. coli 1727 has been analyzed with respect to maintenance energy coefficient (m). The values of ‘m’ for the host, uninduced recombinant and IPTG induced recombinant were determined to be 0.12, 0.17 and 0.32 g.g-1.h-1 respectively. Transient plasmid instability and nearly 33% fall in maximum specific growth rate were observed under conditions of enhanced requirements for maintenance energy.
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Authors and Affiliations
- Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, 110016, Delhi Hauz Khas, New Delhi, India
Sanjoy K Bhattacharya & Ashok K. Dubey
Authors
- Sanjoy K Bhattacharya
- Ashok K. Dubey
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Bhattacharya, S.K., Dubey, A.K. Metabolic burden as reflected by maintenance coefficient of recombinant Escherichia coli overexpressing target gene.Biotechnol Lett 17, 1155–1160 (1995). https://doi.org/10.1007/BF00128377
- Issue date: November 1995
- DOI: https://doi.org/10.1007/BF00128377