An alphoid DNA sequence conserved in all human and great ape chromosomes: evidence for ancient centromeric sequences at human chromosomal regions 2q21 and 9q13 (original) (raw)

• Thomas Ried1,
• Viji Shridhar4,
• Keiko Ogura1,
• Leonardo D'Aiuto5,
• Mariano Rocchi6 &
• …
• David C. Ward1

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Abstract

Using vector-CENP-B box polymerase chain reaction (PCR) we isolated and cloned from a human chromosome 21-specific plasmid library, a 1 kb DNA sequence, named pαH21. In in situ hybridization experiments, pαH21 hybridized, under high stringency conditions, to the centromeric region of all the human, chimpanzee, gorilla and orangutan chromosomes. On human chromosomes pαH21 also identified non-centromeric sequences at 2q21 (locus D2F33S1) and 9q13 (locus D9F33S2). The possible derivation of these sequences from ancestral centromeres is discussed. Sequence analysis confirmed the alphoid nature of the whole pαH21 insert.

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Authors and Affiliations

1. Department of Human Genetics, Yale University School of Medicine, 333 Cedar Street, 06510, New Haven, CT, USA
   Antonio Baldini, Thomas Ried, Keiko Ogura & David C. Ward
2. CNR Institute of Molecular Genetics, Porto Conte Research and Training Laboratories, Alghero, Italy
   Antonio Baldini
3. Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, WC2A 3PX, London, UK
   Antonio Baldini
4. Department of Molecular Biology and Genetics, Wayne State University School of Medicine, Detroit, Michigan, USA
   Viji Shridhar
5. Istituto di Anatomia Umana Normale, Universita'di Modena, Modena, Italy
   Leonardo D'Aiuto
6. Istituto di Genetica, Universita'di Bari, Bari, Italy
   Mariano Rocchi

Authors

1. Antonio Baldini
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2. Thomas Ried
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3. Viji Shridhar
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4. Keiko Ogura
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5. Leonardo D'Aiuto
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6. Mariano Rocchi
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7. David C. Ward
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GenBank accession number, M64321

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Baldini, A., Ried, T., Shridhar, V. et al. An alphoid DNA sequence conserved in all human and great ape chromosomes: evidence for ancient centromeric sequences at human chromosomal regions 2q21 and 9q13.Hum Genet 90, 577–583 (1993). https://doi.org/10.1007/BF00202474

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• Received: 15 April 1992
• Revised: 03 July 1992
• Issue Date: February 1993
• DOI: https://doi.org/10.1007/BF00202474

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