Dynamics of the cytoskeleton of epidermal cells in situ and in culture (original) (raw)
- 56 Accesses
- 21 Citations
- Explore all metrics
Summary
The cytoskeleton of primary tissue-culture cells from the epidermis of Xenopus laevis tadpoles was investigated by phase-contrast, immunofluorescence, and electron microscopy. The connection between the arrangement of different types of filaments and the mechanical properties of the epidermis is discussed. The bilayered epidermis attains stability from thick bundles of tonofilaments interconnecting the basal desmosomes. Twisting of tonofilaments around each other can explain the occurrence of elastic filamentous curls forming a meshwork braced between rows of “small desmosomes” in the apical region of the epidermis. Actin is arranged as a diffuse meshwork and sometimes forms bundles intermingling with tonofilament bundles. Surface membranes and rows of “small desmosomes” are delineated by actin and contain α-actinin. Actin raises the tension for rounding and spreading of cells. Microtubules stabilize already well-developed lamellae.
Access this article
Subscribe and save
- Get 10 units per month
- Download Article/Chapter or eBook
- 1 Unit = 1 Article or 1 Chapter
- Cancel anytime Subscribe now
Buy Now
Price excludes VAT (USA)
Tax calculation will be finalised during checkout.
Instant access to the full article PDF.
Similar content being viewed by others
References
- Bereiter-Hahn J (1967) Dissoziation und Reaggregation von Epidermiszellen der Larven von Xenopus laevis (Daudin) in vitro. Z Zellforsch 79:118–156
Google Scholar - Bereiter-Hahn J, Osborn M, Weber K, Vöth M (1979) Filament organization and formation of microridges at the surface of fish epidermis. J Ultrastruct Res 69:316–330
Google Scholar - Goldman RJ (1971) The role of three cytoplasmic fibres in BHK-21 cell motility. 1. Microtubules and the effect of colchicine. J Cell Biol 51:752–762
Google Scholar - Gordon WE, Bushnell A, Burridge K (1978) Characterization of the intermediate (10 nm) filaments of cultured cells using an autoimmune rabbit antiserum. Cell 13:249–261
Google Scholar - Heidenhain M (1899) Über die Struktur der Darmepithelzellen. Arch Mikr Anat Entwgsch 54:184–224
Google Scholar - Henderson D, Weber K (1981) Immuno-electron microscopical identification of the two types of intermediate filaments in established epithelial cells. Exp Cell Res 132:297–311
Google Scholar - Jarosch R (1966) On the behavior of rotating helices. In: Warren KB (ed) Intracellular transport. Academic Press Inc, New York, pp 275–300
Google Scholar - Nieuwkoop PD, Faber J (1956) Normal table of Xenopus laevis (Daudin). North-Holland, Amsterdam
Google Scholar - Radice GP (1980a) The spreading of epithelial cells during wound closure in Xenopus larvae. Dev Biol 76:26–46
Google Scholar - Radice GP (1980b) Locomotion and cell-substratum contacts of Xenopus epidermal cells in vitro and in situ. J Cell Sci 44:201–223
Google Scholar - Rees DA, Lloyd CW, Thom D (1977) Control of grip and stick in cell adhesion through lateral relationships of membrane glycoproteins. Nature 267:124–128
Google Scholar - Rees DA, Badley RA, Lloyd CW, Thom D, Smith CG (1978) Glycoproteins in the recognition of substratum by cultured fibroblasts. In: Curtis ASG (ed) Cell-cell recognition. Cambridge University Press, Cambridge, pp 241–260
Google Scholar - Rose GG, Pomerat CM, Shindler TO, Trunnel JB (1958) A cellophane-strip technique for culturing tissue in multipurpose culture chambers. J Biophys Biochem Cytol 4:761–764
Google Scholar - Schliwa M (1975) Cytoarchitecture of surface layer cells of the teleost epidermis. J Ultrastruct Res 52:377–386
Google Scholar - Schliwa M (1981) Structural interactions in the cytoskeleton. Biophys J 33:129a
Google Scholar - Wainwright SA, Biggs WD, Currey JD, Gosline JM (1976) Mechanical design in organisms. Arnold, London, p 133
Google Scholar - Weber K, Bibring T, Osborn M (1975) Specific visualization of tubulin containing structures in tissue culture cells by immunofluorescence. Exp Cell Res 95:111–120
Google Scholar - Weber K, Wehland J, Herzog W (1976) Griseofulvin interacts with microtubules both in vivo and in vitro. J Mol Biol 102:817–829
Google Scholar - Webster RE, Osborn M, Weber K (1978) Visualization of the same PtK2 cytoskeletons by both immunofluorescence and low power electron microscopy. Exp Cell Res 117:47–61
Google Scholar - Weiß P (1961) The biological foundations of wound repair. In: The Harvey Lectures 55 (1959–1960). Academic Press, New York, pp 13–42
Google Scholar
Author information
Authors and Affiliations
- AK Kinematische Zellforschung, J.W. Goethe-Universität, Frankfurt am Main
Inge Kunzenbacher & Jürgen Bereiter-Hahn - Max-Planck-Institut für Biophysikalische Chemie, Göttingen, Bundesrepublik Deutschland
Mary Osborn & Klaus Weber
Authors
- Inge Kunzenbacher
You can also search for this author inPubMed Google Scholar - Jürgen Bereiter-Hahn
You can also search for this author inPubMed Google Scholar - Mary Osborn
You can also search for this author inPubMed Google Scholar - Klaus Weber
You can also search for this author inPubMed Google Scholar
Rights and permissions
About this article
Cite this article
Kunzenbacher, I., Bereiter-Hahn, J., Osborn, M. et al. Dynamics of the cytoskeleton of epidermal cells in situ and in culture.Cell Tissue Res. 222, 445–457 (1982). https://doi.org/10.1007/BF00213224
- Accepted: 27 October 1981
- Issue Date: January 1982
- DOI: https://doi.org/10.1007/BF00213224