Development of dome epithelium in gut-associated lymphoid tissues: Association of IgA with M cells (original) (raw)
Summary
The dome epithelium (DE), which covers gutassociated lymphoid tissues (GALT) and provides both a protective barrier over lymphoid follicles and a route for antigen uptake from the gut, develops in rabbit appendix (caecum) during the first week of neonatal life. To determine if secretory immunoglobulins from maternal milk interact with this developing tissue, their interrelationships in neonatal rabbit appendix were examined by use of immunocytochemical techniques. The glycoprotein, secretory component, was not produced by neonatal rabbits less than 15 days old, since neither the membranous nor the free, secreted forms of maternal secretory component were associated with villi or DE of neonates. Immunoglobulin A (IgA), but neither IgG nor IgM, were noted on DE by light microscopy, even though IgG was abundant in the villus lamina propria and vascular spaces. The epithelial IgA was distributed, in a patchy pattern, across the upper dome surface of some two-day-old, and all five-and ten-day old nursing animals, but IgA was not on DE of rabbits prevented from nursing. Immunoelectron microscopy of appendix from nursed rabbits revealed IgA directly over the apical surface of M cells, where it formed a continous, thick coating without binding to adjacent immature absorptive cells; it was also within apical vacuoles of M cell cytoplasm. The distribution of IgA on the DE of rabbit appendices indicated that in differentiating GALT, maternal IgA reacted preferentially with M cells or pre-M cells, leading to speculation concerning a role for IgA in the development of GALT and in establishment of mucosal immune responses in neonates.
In conducting the research described in this report, the investigators adhered to the standards set forth in the “Guide for the Care and Use of Laboratory Animals” (NIH Publication 85-23) as promulgated by the Committee on Care and Use of Laboratory Animals of the Institute of Laboratory Animal Resources, National Research Council, USA
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Abbreviations
DE:
dome epithelium
GALT:
gutassociated lymphoid tissues
HRP:
horseradish peroxidase
IgA:
immunoglobulin A
SC:
secretory component
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Authors and Affiliations
- Department of Experimental Pathology, Walter Reed Army Institute of Research, Washington, DC, USA
Michael J. Roy & M. Varvayanis
Authors
- Michael J. Roy
- M. Varvayanis
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The views of the authors expressed here do not purport to reflect the position of the Department of the Army or the Department of Defense
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Roy, M.J., Varvayanis, M. Development of dome epithelium in gut-associated lymphoid tissues: Association of IgA with M cells.Cell Tissue Res. 248, 645–651 (1987). https://doi.org/10.1007/BF00216495
- Accepted: 08 September 1986
- Issue date: June 1987
- DOI: https://doi.org/10.1007/BF00216495