Advanced backcross QTL analysis in a cross between an elite processing line of tomato and its wild relative L. pimpinellifolium (original) (raw)
Abstract
Approximately 170 BC2 plants from a cross between an elite processing inbred (recurrent parent) and the wild species Lycopersicon pimpinellifolium LA1589 (donor parent) were analyzed with segregating molecular markers covering the entire tomato genome. Marker data were used to identify QTLs controlling a battery of horticultural traits measured on BC2F1 and BC3 families derived from the BC2 individuals. Despite its overall inferior appearance, L. pimpinellifolium was shown to possess QTL alleles capable of enhancing most traits important in processing tomato production. QTL-NIL lines, containing specific QTLs modifying fruit size and shape, were subsequently constructed and shown to display the transgressive phenotypes predicted from the original BC2 QTL analysis. The potential of exploiting unadapted and wild germplasm via advanced backcross QTL analysis for the enhancement of elite crop varieties is discussed.
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Authors and Affiliations
- Department of Plant Breeding and Biometry, Cornell University, 252 Emerson Hall, 14853, Ithaca, NY, USA
S. D. Tanksley, S. Grandillo & T. M. Fulton - The Faculty of Agriculture; Department of Field Crops, Hebrew University, Kennedy Lee Building; Rm 222, Box 12, 76-100, Rehovot, Israel
D. Zamir & Y. Eshed - Francereco, 101 Avenue Gustave Eiffel, 37390, Notre Dame D'OE, Tours, France
V. Petiard - Hispareco S.A.; Centro de Investigacion y Desarrollo en Alimentacion, Apartado 435, E-06080, Badajoz, Spain
J. Lopez - Peto Seeds, 37437 State Highway 16, 95695, Woodland, CA, USA
T. Beck-Bunn
Authors
- S. D. Tanksley
- S. Grandillo
- T. M. Fulton
- D. Zamir
- Y. Eshed
- V. Petiard
- J. Lopez
- T. Beck-Bunn
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Communicated by G. Wenzel
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Tanksley, S.D., Grandillo, S., Fulton, T.M. et al. Advanced backcross QTL analysis in a cross between an elite processing line of tomato and its wild relative L. pimpinellifolium.Theoret. Appl. Genetics 92, 213–224 (1996). https://doi.org/10.1007/BF00223378
- Received: 05 July 1995
- Accepted: 04 August 1995
- Issue date: February 1996
- DOI: https://doi.org/10.1007/BF00223378