Inheritance of chloroplast and mitochondrial DNA in Picea and composition of hybrids from introgression zones (original) (raw)
Summary
The cloning of white spruce (Picea glauca) mitochondrial DNA homologous to the cytochrome oxidase II and ATPaseα genes of maize is described. These probes were used to define restriction fragment length polymorphisms which distinguish the white, Engelmann (P. engelmannii) and Sitka spruce (P. sitchensis) populations that occur in British Columbia. Analysis of progeny from crosses between the species revealed that mitochondrial DNA was maternally inherited in all cases (32 progeny from five independent crosses). The inheritance of chloroplast DNA was determined using a probe described previously; in this case, all progeny exhibited paternal inheritance (27 progeny from four crosses). Mitochondrial and chloroplast probes were used to test trees from zones of introgression between coastal (Sitka) and interior spruces (white and Engelmann). In most cases mitochondria and chloroplasts within individuals were contributed by different species. The data shows that there is a significant Sitka spruce component in trees east of the coastal watershed in British Columbia.
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References
- Brix H (1972) Growth response of Sitka spruce and white spruce seedlings to temperature and light intensity. Can For Serv, Pac For Res Cent, Inf Rep BC-X-74
- Braun CJ, Levings III CS (1985) Nucleotide sequence of the F1-ATPaseα subunit gene from maize mitochondria. Plant Physiol 79:571–577
Google Scholar - Daubenmire R (1968) Taxonomic and ecological relationships between Picea glauca and Picea sitchensis and their ecological interpretation. Can J Bot 46:787–798
Google Scholar - Feinberg AP, Vogelstein B (1983) A technique for radiolabelling of restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
CAS PubMed Google Scholar - Feinberg AP, Vogelstein B (1984) Addendum to “A technique for radiolabelling of restriction endonuclease fragments to high specific activity”. Anal Biochem 137:266–267
CAS PubMed Google Scholar - Fox TD, Leaver CJ (1981) The Zea mays mitochondrial gene coding cytochrome oxidase subunit II has an intervening sequence and does not contain TGA codons. Cell 26:315–323
Google Scholar - Krajina VT, Klinka K, Worrall J (1982) Distribution and ecological characteristics of trees and shrubs of British Columbia. UBC Fac For Publ
- Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
Google Scholar - Neale DB, Sederoff RR (1989) Paternal inheritance of chloroplast DNA and maternal inheritance of mitochondrial DNA in loblolly pine. Theor Appl Genet 77:212–216
Google Scholar - Neale DB, Wheeler NC, Allard RW (1986) Paternal inheritance of chloroplast DNA in Douglas-fir. Can J For Res 6:1152–1154
Google Scholar - Neale DB, Marshall KA, Sederoff RR (1989) Chloroplast and mitochondrial DNA are paternally inherited in Sequoia sempervirens. DDon. Endl. Proc Natl Acad Sci USA 86:9347–9349
Google Scholar - Palmer JD (1987) Chloroplast DNA evolution and biosystematic uses of chloroplast DNA variation. Am Nat 130:S6-S29
Article CAS Google Scholar - Pay A, Smith MA (1988) A rapid method for the purification of organelles for DNA isolation: self-generated percoll gradients. Plant Cell Rep 7:96–99
Google Scholar - Roche L (1969) A genecological study of the genus Picea in British Columbia. New Phytol 68:505–554
Google Scholar - Sederoff RR (1987) Molecular mechanisms of mitochondrial genome evolution. Am Nat 130:S830-S45
Google Scholar - Sutton BCS, Flanagan DJ, El-Kassaby YA (1991) A simple and rapid method for estimating representation of species in spruce seedlots using chloroplast DNA restriction fragment length polymorphism. Silvae Genet (in press)
- Szmidt AE, Alden T, Hallgren JE (1987) Paternal inheritance of chloroplast DNA in Larix. Plant Mol Biol 9:59–64
Google Scholar - Szmidt AE, El-Kassaby YA, Sigurgeirsson A, Alden T, Lindgren D, Hallgren JE (1988) Classifyig seedlots of Picea sitchensis and P. glauca in zones of introgression, using restriction analysis of chloroplast DNA. Theor Appl Genet 76:841–845
Google Scholar - Wagner DB, Furnier GR, Saghai-Maroof MA, Williams SM, Dancik BP, Allard RW (1987) Chloroplast DNA polymorphism in lodgepole and jack pines and their hybrids. Proc Natl Acad Sci USA 84:2097–2100
CAS PubMed Google Scholar - Webb DT, Webster F, Flinn BS, Roberts DR, Ellis DE (1989) Factors influencing the induction of embryogenic and caulogenic callus from embryos of Picea glauca and P. engelmannii. Can J For Res 19:1303–1308
Google Scholar - White EE (1990) Chloroplast DNA in Pinus monticola: 2. Survey of within species variability and detection of heteroplasmic individuals. Theor Appl Genet 79:251–255
Google Scholar
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Authors and Affiliations
- Forest Biotechnology Centre, British Columbia Research Corporation, 3650 Wesbrook Mall, V6S 2L2, Vancouver, B.C., Canada
B. C. S. Sutton, D. J. Flanagan, J. R. Gawley & C. H. Newton - Forest Science Department, University of British Columbia, 2357 Main Mall, V6T 1W5, Vancouver, B.C., Canada
B. C. S. Sutton, D. T. Lester & Y. A. El-Kassaby - Saanich Forestry Centre, Canadian Pacific Forest Products Ltd, 8067 East Saanich Rd, V0S 1M0, RR1, Saanichton, B.C., Canada
Y. A. El-Kassaby
Authors
- B. C. S. Sutton
- D. J. Flanagan
- J. R. Gawley
- C. H. Newton
- D. T. Lester
- Y. A. El-Kassaby
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Communicated by P.M.A. Tigerstedt
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Sutton, B.C.S., Flanagan, D.J., Gawley, J.R. et al. Inheritance of chloroplast and mitochondrial DNA in Picea and composition of hybrids from introgression zones.Theoret. Appl. Genetics 82, 242–248 (1991). https://doi.org/10.1007/BF00226220
- Received: 19 December 1990
- Accepted: 08 January 1991
- Issue date: August 1991
- DOI: https://doi.org/10.1007/BF00226220