Genetic diversity at isozyme and RFLP loci in Brassica campestris as related to crop type and geographical origin (original) (raw)
Summary
Twenty accessions of Brassica campestris, representing the major crop types and their geographical origin, were tested for gene frequency at five isozyme and four RFLP loci. The majority of alleles (67%) were found in all geographic regions. Nearly 3 times more alleles were detected at RFLP loci than at isozyme loci. Genetic diversity among crop types (with the exception of turnip) was similar to diversity estimates of geographical regions, implying that crops used for similar purposes (i.e., oilseed or leafy vegetable) are derived from geographically differentiated populations. Geographically, Central Asian and Indian types showed the highest level of heterozygosity (excluding self-fertile sarson oilseed types), followed closely by European varieties, and Asian varieties showed the greatest gene diversity. Phenetic dendrograms indicated that sarson and Chinese cabbage have diverged considerably from other types, perhaps due to consequences of their breeding habit or origin.
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References
- Arus P, Orton TJ (1983) Inheritance and linkage relationships of isozyme loci in Brassica oleracea. J Hered 74:405–412
Google Scholar - Chen BY, Heneen WK, Simonsen V (1990) Genetics of isozyme loci in Brassica campestris L. and in the progeny of a trigenomic hybrid between B. napus L. and Brassica campestris L. Genome 33:433–440
Google Scholar - De Candolle A (1886) Origin of cultivated plants. Hafner Publ Co, New York 1964 reprint
Google Scholar - Denford KE, Vaughan JG (1977) A comparative study of certain seed isoenzymes in the ten chromosome complex of Brassica campestris and its allies. Ann Bot 41:411–418
Google Scholar - Downey RK, Stringam GR, McGregor DI, Stefansson BR (1975) Breeding rapeseed and mustard crops. In: Harapiak JT (ed) Oilseed and pulse crops in Western Canada: a symposium. Western Co-operative Fertilizers, Calgary, pp 157–183
Google Scholar - Felsenstein J (1981) Evolutionary trees from gene frequencies and quantitative characters: finding maximum likelihood estimates. Evolution 35:1229–1242
Google Scholar - Li CW (1981) The origin, evolution, taxonomy and hybridization of Chinese cabbage. In: Talekar NS, Griggs TD (eds) Chinese cabbage: Proc. 1st Int Symp Asian Veg Res Dev Center. Taiwan, pp 3–9
- Li H-L (1969) The vegetables of ancient China. Econ Bot 23:253–260
Google Scholar - Li H-L (1983) The domestication of plants in China: ecogeographical considerations. In: Keightley DN (ed) The origins of Chinese civilization. University of California Press, Berkeley, California, pp 21–63
Google Scholar - McGrath JM, Quiros CF (1991a) Inter-subspecies hybrids and their progeny in Brassica campestris L. J Am Soc Hortic Sci 116:349–355
Google Scholar - McGrath JM, Quiros CF (1991b) Inheritance of isozyme and RFLP markers in Brassica campestris and comparison with B. oleracea. Theor Appl Genet 82:668–673
Google Scholar - McGrath JM, Quiros CF, Harada JJ, Landry BS (1990) Identification of Brassica oleracea monosomic alien chromosome addition lines with molecular markers reveals extensive gene duplication. Mol Gen Genet 223:198–204
Google Scholar - Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York
Google Scholar - Nishi S (1980) Differentiation of Brassica crops in Asia and the breeding of ‘Hakuran’, a newly synthesized leafy vegetable. In: Tsunoda S, Hinata K, Gomez-Campo C (eds) Brassica) crops and wild allies: biology and breeding. Jpn Sci Soc Press, Tokyo, pp 133–150
Google Scholar - Olsson G (1954) Crosses within the campestris group of the genus Brassica. Hereditas 40:398–418
Google Scholar - Palmer JD, Shields CR, Cohen DB, Orton TJ (1983) Chloroplast DNA evolution and the origin of amphidiploid Brassica species. Theor Appl Genet 65:181–189
Google Scholar - Prakash S, Hinata K (1980) Taxonomy, cytogenetics and origin of crop Brassicas, a review. Opera Bot 55:1–57
Google Scholar - Quiros CF (1987) Duplicated isozyme loci and cellular compartmentalization of their products in Brassica. Cruciferae Newsl 12:24
Google Scholar - Quiros CF, Ochoa O, Kianian S, Douches D (1987) Analysis of the Brassica oleracea genome by the generation of B. campestris-oleracea chromosome addition lines. Theor Appl Genet 74:758–766
Google Scholar - Renfrew JM (1973) Paleoethnobotany: the prehistoric food plants of the Near East and Europe. Columbia Universiy Press, New York
Google Scholar - Sinskaia EN (1927) Geno-systemical investigations of cultivated Brassica. Bull Appl Bot Plant Breed 17:1–166
Google Scholar - Sinskaia EN (1928) The oleiferous plants and root crops of the family Cruciferae. Bull Appl Bot Plant Breed 19:1–648
Google Scholar - Song KM, Osborn TC, Williams PH (1990) Brassica taxonomy based on nuclear restriction fragment polymorphisms (RFLPs). 3. Genome relationships in Brassica and related genera and the origin of B. oleracea and B. rapa (syn. campestris). Theor Appl Genet 79:497–506
Google Scholar - Truco MJ (1986) Estudie de la Variabilitat isoenzimatica en Brassica campestris. Thesis, University of Barcelona, Spain
Google Scholar - Werner K (1984) New turnip greens variety wins grower fans. Am Vegetable Grower32:16–17
Google Scholar - Whyte RO (1983) The evolution of the Chinese environment. In: Keightley DN(ed) The origins of Chinese civilization. University of California Press, Berkeley, Calif., pp 3–19
Google Scholar - Yarnell SH (1956) Cytogenetics of the vegetable crops. II. Crucifers. Bot Rev 22:81–166
Google Scholar - Zohary D, Hopf M (1988) Domestication of plants in the old world. Clarendon Press, Oxford
Google Scholar
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- J. Mitchell McGrath
Present address: Department of Biology, University of Michigan, 48109-1048, Ann Arbor, MI, USA
Authors and Affiliations
- Department of Vegetable Crops, University of California-Davis, 95616, CA, USA
J. Mitchell McGrath & Carlos F. Quiros
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- J. Mitchell McGrath
You can also search for this author inPubMed Google Scholar - Carlos F. Quiros
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Communicated by H. F. Linskens
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McGrath, J.M., Quiros, C.F. Genetic diversity at isozyme and RFLP loci in Brassica campestris as related to crop type and geographical origin.Theoret. Appl. Genetics 83, 783–790 (1992). https://doi.org/10.1007/BF00226698
- Received: 26 July 1991
- Accepted: 03 September 1991
- Issue Date: April 1992
- DOI: https://doi.org/10.1007/BF00226698