Immunofluorescent localization of insulin-like material in the median neurosecretory cells of the blowfly, Calliphora vomitoria (Diptera) (original) (raw)
Summary
Immunocytochemical staining has shown that the median neurosecretory cells (MNC) of the brain of the blowfly, Calliphora vomitoria, contain an insulin-like material which cross reacts with antibodies to bovine insulin. There are 24–26 paraldehyde fuchsin-positive MNC of which only 6–8 show the specific insulin-like immunoreactivity.
Access this article
Subscribe and save
- Starting from 10 chapters or articles per month
- Access and download chapters and articles from more than 300k books and 2,500 journals
- Cancel anytime View plans
Buy Now
Price excludes VAT (USA)
Tax calculation will be finalised during checkout.
Instant access to the full article PDF.
Similar content being viewed by others
References
- Barrington, E.J.W.: Evolutionary aspects of hormonal structure and function. In: Comparative Endocrinology (Gaillard, P.J., Boer, H.H., eds.) 381–396. Amsterdam: Elsevier/North Holland Biomedical Press 1978
Google Scholar - Bloch, B., Thomsen, E., Thomsen, M.: The neurosecretory system of the adult Calliphora erythrocephala. III. Electron microscopy of the medial neurosecretory cells of the brain and some adjacent cells. Z. Zellforsch. 70, 185–208 (1966)
Google Scholar - Chen, A.C., Friedman, S.: Hormonal regulation of trehalose metabolism in the blowfly, Phormia regina: Interaction between hypertrehalosemic and hypotrehalosemic hormones. J. Insect Physiol. 23, 1223–1232 (1977)
Google Scholar - Coons, A.H., Leduc, E.H., Connolly, J.M.: Studies on antibody production. I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit. J. Exp. Med. 102, 49–59 (1955)
Google Scholar - Duve, H.: The presence of a hypoglucemic and hypotrehalocemic hormone in the neurosecretory system of the blowfly, Calliphora erythrocephala. Gen. Comp. Endocrinol. 36, 102–110 (1978)
Google Scholar - Duve, H., Thorpe, A., Lazarus, N.: The isolation of material displaying insulin-like immunological and biological activity from the brain of the blowfly, Calliphora vomitoria. Biochem. J. (In press)
- Guillemin, R.: The expanding significance of hypothalamic peptides, or, is endocrinology a branch of neuroendocrinology? Recent Prog. Horm. Res. 33, 1–28 (1977)
Google Scholar - Panov, A.A.: The composition of the medial neurosecretory cells of the pars intercerebralis in Calliphora and Lucilia adults. Zool. Anz. Jena 196, 23–27 (1976)
Google Scholar - Pearse, A.G.E.: The cytochemistry and ultrastructure of polypeptide hormone producing cells of the APUD series and the embryologic, physiologic and pathologic implication of the concept. J. Histochem. Cytochem. 17, 303–313 (1969)
Google Scholar - Pearse, A.G.E., Takor Takor, T.: Neuroendocrine embryology and the APUD concept. Clinical Endocrinology 5, Suppl. 229–244 (1976)
Google Scholar - Tager, H.S., Markese, J., Kramer, K.J., Speirs, R.D., Childs, C.N.: Glucagon-like and insulin-like hormones of the insect neurosecretory system. Biochem. J. 156, 515–520 (1976)
Google Scholar - Thomsen, M.: I. The neurosecretory system of the adult Calliphora erythrocephala. II. Histology of the neurosecretory cells of the brain and some related structures. Z. Zellforsch. 67, 693–717 (1965)
Google Scholar - Thomsen, E., Lea, A.O.: Control of the medial neurosecretory cells by the corpus allatum in Calliphora erythrocephala. Gen. Comp. Endocrinol. 12, 51–57 (1969)
Google Scholar - Weyer, F.: Über drüsenartige Nervenzellen im Gehirn der Honigbiene, Apis mellifica L. Zool. Anz. 112, 137–141 (1935)
Google Scholar
Author information
Authors and Affiliations
- Institute of General Zoology, University of Copenhagen, Universitetsparken 15, DK-2100, Copenhagen Ø, Denmark
Hanne Duve & Alan Thorpe - Department of Zoology and Comparative Physiology, Queen Mary College, London University, London, England
Alan Thorpe
Authors
- Hanne Duve
- Alan Thorpe
Additional information
Dr. M.C. Thorndyke, Department of Zoology, Bedford College, London University, kindly provided us with fluorescence facilities, and we are also grateful to him for helpful discussions on the immunofluorescence procedure
Rights and permissions
About this article
Cite this article
Duve, H., Thorpe, A. Immunofluorescent localization of insulin-like material in the median neurosecretory cells of the blowfly, Calliphora vomitoria (Diptera).Cell Tissue Res. 200, 187–191 (1979). https://doi.org/10.1007/BF00236411
- Accepted: 14 March 1979
- Issue date: August 1979
- DOI: https://doi.org/10.1007/BF00236411