Phage P22-mutants with increased or decreased transduction abilities (original) (raw)

Summary

The properties of mutants of the Salmonella phage P22 are described which show decreased or increased frequencies for generalized transduction. It is shown that not only the markers used for detection of the mutants are affected by the mutation but also other markers tested. Evidence is presented that the altered T/P-ratios reflect changes in the actual numbers of transducing particles and that other factors like integration in the recipient cells cannot account for these alterations. Quantitative estimations of the amount of bacterial DNA converted to transducing fragments are shown for several phage mutants. Difficulties in the isolation procedure are discussed.

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Authors and Affiliations

1. Abt. Molekulare Genetik, Institut für Mikrobiologie der Gesellschaft für Strahlen- und Umweltforschung, Göttingen, West-Germany
   Horst Schmieger

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1. Horst Schmieger
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Communicated by P. Starlinger

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Schmieger, H. Phage _P22_-mutants with increased or decreased transduction abilities.Molec. Gen. Genet. 119, 75–88 (1972). https://doi.org/10.1007/BF00270447

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• Received: 21 June 1972
• Issue Date: March 1972
• DOI: https://doi.org/10.1007/BF00270447

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