Comparison of 35S- and digoxigenin-labeled RNA and oligonucleotide probes for in situ hybridization (original) (raw)
References
Baldino F Jr, Lewis ME (1989) Nonradioactive in situ hybridization histochemistry with digoxigenin-deoxyuridine 5′-triphosphate-labeled oligonucleotides. In: Conn PM (ed) Gene probes. Methods in neuroscience. Academic Press, San Diego, pp 282–292 Google Scholar
Chang C, Kokontis J, Liao S (1988) Structural analysis of complementary DNA and amino acid sequences of human and rat androgen receptors. Proc Natl Acad Sci USA 85:7211–7215 Google Scholar
Cook AF, Voucolo E, Brakel CL (1988) Synthesis and hybridization of biotinylated oligonucleotides. Nucleic Acids Res 16:4077–4095 Google Scholar
Dodd JG, Kreis Ch, Sheppard PC, Hamel A, Matusik RJ (1986) Effect of androgens on mRNA for a secretory protein of dorsolateral prostate and seminal vesicles. Mol Cell Endocrinol 47:191–200 Google Scholar
Farquharson M, Harvie R, McNicol AM (1990) Detection of messenger RNA using a digoxigenin end labelled oligodeoxynucleotide probe. J Clin Pathol 43:424–428 Google Scholar
Forster AC, McInnes JL, Skingle JC, Symons RH (1985) Nonradioactive hybridization probes prepared by chemical labeling of DNA and RNA with a novel reagent, photobiotin. Nucleic Acids Res 13:745–761 Google Scholar
Furuta Y, Shinohara T, Sano K, Meguro M, Nagashima K (1990) In situ hybridization with digoxigenin-labelled DNA probes for detection of viral genomes. J Clin Pathol 43:806–809 Google Scholar
Gall JG, Pardue ML (1971) Nucleic acid hybridization in cytological preparations. Methods Enzymol 38:470–480 Google Scholar
Gerfen CR (1989) Quantification of in situ hybridization histochemistry for analysis of brain function. In: Conn PM (ed) Gene probes. Methods in neuroscience. Academic Press, San Diego, pp 79–97 Google Scholar
Harris SE, Harris MA, Johnson CM, Bean MF, Dodd JG, Matusik RJ, Carr SA, Crabb JW (1990) Structural characterization of the rat seminal vesicle secretion II protein and gene. J Biol Chem 265:9896–9903 Google Scholar
Heiles HBJ, Genersch E, Kessler C, Neumann R, Eggers HJ (1988) In situ hybridization with digoxigenin-labeled DNA of human papillomaviruses (HPV16/18) in HeLa and SiHa cells. BioTechniques 6:978–981 Google Scholar
Heino P, Hukkanen V, Arstila P (1989) Detection of human papilloma viruses (HPV) DNA in genital biopsy specimens by in situ hybridization with digoxigenin-labeled probes. J Virol Methods 26:331–337 Google Scholar
Höfler H, Childers H, Montminy MR, Lechan RM, Goodmann RH, Wolfe HJ (1986) In situ hybridization methods for the detection of somatostatin mRNA in tissue sections using anti-sense RNA probes. Histochem J 18:597–604 Google Scholar
Hopman AHN, Wiegant J, Van Duijn P (1986) A new hybridocyto-chemical method based on mercurated nucleic acid probes and sulfhydryl-hapten ligands. I. Stability of mercury-sulfhydryl bond and influence of the ligand structure on immunochemical detection of the hapten. Histochemistry 84:169–178 Google Scholar
Jesik CJ, Holland JM, Lee C (1982) An anatomic and histologic study of the rat prostate. The Prostate 3:81–97 Google Scholar
Kendall CH, Roberts PA, Pringle JH, Lauder I (1991) The expression of parathyroid hormone messenger RNA in normal and abnormal parathyroid tissue. J Pathol 165:111–118 Google Scholar
Komminoth P (1992) Digoxigenin as an alternative probe labeling for in situ hybridization. Diagn Mol Pathol 1:142–150 Google Scholar
Lackie PM, Zuber C, Roth J (1991) Expression of polysialylated N-CAM during rat heart development. Differentiation 47:85–98 Google Scholar
Langer PR, Waldrop AA, Ward DC (1981) Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes. Proc Natl Acad Sci USA 78:6633–6637 Google Scholar
Larsson L-I, Hougaard DM (1992) Detection of gastrin and its messenger RNA in Zollinger-Ellison tumors by non-radioactive in situ hybridization and immunocytochemistry. Histochemistry 97:105–110 Google Scholar
Long AA, Mueller J, Andre-Schwartz J, Barrett KJ, Schwartz R, Wolfe HJ (1992) High-specificity in situ hybridization: methods and application. Diagn Mol Pathol 1:45–57 Google Scholar
Matusik RJ, Kreis C, McNicol P, Sweetland R, Mullin C, Fleming WH, Dodd JG (1986) Regulation of prostatic genes: role of androgens and zinc in gene expression. Biochem Cell Biol 64:601–607 Google Scholar
Morimoto H, Monden T, Shimano T, Higashiyama M, Tamita N, Murotani M, Matsuura N, Okuda H, Mori T (1987) Use of sulfonated robes for in situ detection of amylase mRNA in formalin-fixed paraffin sections of human pancreas and submaxillary gland. Lab Invest 57:737–741 Google Scholar
Morris RG, Arends MJ, Bishop PE, Sizer K, Duvall E, Bird CC (1990) Sensitivity of digoxigenin and biotin labelled probes for detection of human papillomavirus by in situ hybridization. J Clin Pathol 43:800–805 Google Scholar
Nakane PK, Koji T, Izumi S (1989) In situ localization of mRNA with T-T dimerized DNA probes. Hum Cell 2:356–362 Google Scholar
Niedobitek G, Finn T, Herbst H, Bornhöft G, Gerdes J, Stein H (1988) Detection of viral DNA by in situ hybridization using bromodeoxyuridine-labeled DNA probes. Am J Pathol 131:1–4 Google Scholar
Price D (1963) Comparative aspects of development and structure in the prostate. In: Vollmer EP (ed) Biology of the prostate and related tissues. US Department of Health, Education and Welfare, Bethesda, Md, pp 1–27 Google Scholar
Roth J (1989) Postembedding labeling on Lowicryl K4M tissue sections: detection and modification of cellular components. Methods Cell Biol 31:513–551 Google Scholar
Roth J, Bendayan M, Orci L (1978) Ultrastructural localization of intracellular antigens by the use of protein A-gold complex. J Histochem Cytochem 26:1074–1081 Google Scholar
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY Google Scholar
Sata T, Zuber C, Roth J (1990) Lectin-digoxigenin conjugates: a new hapten system for glycoconjugate cytochemistry. Histochemistry 94:1–11 Google Scholar
Shan LX, Rodriguez MC, Janne OA (1990) Regulation of androgen receptor protein and mRNA concentration by androgens in rat ventral prostate and seminal vesicles and in human hepatoma cells. Mol Endocrinol 4:1636–1646 Google Scholar
Shroyer KP, Nakane PKJ (1983) Use of DNP-labeled cDNA for in situ hybridization. J Cell Biol 97:377a Google Scholar
Takeda H, Chodak G, Mutchnik S, Nakamoto T, Chang C (1990) Immunohistochemical localization of androgen receptors with mono and polyclonal antibodies to androgen receptor. J Endocrinol 126:17–25 Google Scholar
van den Brule AJC, Cromme FV, Snijders PJF, Smit L, Oudejans CBM, Baak JPA, Meijer CJLM, Waldboomers JMM (1991) Nonradioactive RNA in situ hybridization detection of human papillomavirus 16-E7 transcripts in squamous cell carcinomas of the uterine cervix using confocal laser scan microscopy. Am J Pathol 139:1037–1045 Google Scholar
Venole P (1990) Digoxigenin-labeled probes can detect single-copy genes in human metaphase chromosomes. BioTechniques 9:200–204 Google Scholar
Yu M, Leav BA, Leav I, Merk FB, Wolfe HJ, Ho S-M (1993) Early alterations in ras protooncogene mRNA expression in testosterone and estradiol-17β induced prostatic dysplasia of Noble rats. Lab Invest 68 (in press)