Characterization of the cleavage site and the recognition sequence of the I-CreI DNA endonuclease encoded by the chloroplast ribosomal intron of Chlamydomonns reinhardtii (original) (raw)
Summary
The chloroplast ribosomal intron of Chlamydomonas reinhardtii encodes a sequence-specific DNA endonuclease (I-_Cre_I), which is most probably involved in the mobility of this intron. Here we show that I-_Cre_I generates a 4 by staggered cleavage just downstream of the intron insertion site. The I-_Cre_I recognition sequence is 19–24 by in size and is located asymmetrically around the intron insertion site. Screening of natural variants of the I-_Cre_I recognition sequence indicates that the I-_Cre_I endonuclease tolerates single and even multiple base changes within its recognition sequence.
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Authors and Affiliations
- Departments of Molecular Biology and Plant Biology, University of Geneva, 30 quai Ernest-Ansermet, 1211, Genève 4, Switzerland
Franz Dürrenberger & Jean-David Rochaix
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- Franz Dürrenberger
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Communicated by R.G. Herrmann
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Dürrenberger, F., Rochaix, JD. Characterization of the cleavage site and the recognition sequence of the I-_Cre_I DNA endonuclease encoded by the chloroplast ribosomal intron of Chlamydomonns reinhardtii.Molec. Gen. Genet. 236, 409–414 (1993). https://doi.org/10.1007/BF00277141
- Received: 23 June 1992
- Accepted: 10 August 1992
- Issue Date: January 1993
- DOI: https://doi.org/10.1007/BF00277141