Sequence complexity of the S receptor kinase gene family in Brassica (original) (raw)

Abstract

A genomic library from an S 29/S 29 self-incompatible genotype of Brassica oleracea was screened with a probe carrying part of the catalytic domain of a _Brassica S_-receptor kinase (SRK)-like gene. Six positive phage clones with varying hybridisation intensities (K1 to K6) were purified and characterised. A 650–700 by region corresponding to the probe was excised from each clone and sequenced. DNA and predicted protein sequence comparisons based on a multiple alignment identified K5 as a pseudogene, whereas the others could encode functional proteins. K3 was found to have lost an intron from its genomic sequence. The six genes display different degrees of sequence similarity and form two distinct clusters in a dendrogram. The 98% similarity between K4 and K6, which extends across intron sequences, suggests that these might be very recently diverged alleles or daughters of a duplication. In addition, K2 showed a comparably high similarity to the probe. Clones K1, K3 and K5 cross-hybridised with an SLG 29 cDNA probe, indicating the presence of upstream receptor domains homologous to the Brassica SLG gene. This suggests that the previously reported S sequence complexity may be ascribed to a large receptor kinase gene family.

Access this article

Log in via an institution

Subscribe and save

• Starting from 10 chapters or articles per month
• Access and download chapters and articles from more than 300k books and 2,500 journals
• Cancel anytime View plans

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

• Bairoch A, Claverie J-M (1988) Sequence patterns in protein kinases. Nature 331:22
  Google Scholar
• Bateman AJ (1955) Self-incompatibility systems in angiosperms. III. Cruciferae. Heredity 9:53–68
  Google Scholar
• Boyes DC, Nasrallah JB (1993) Physical linkage of the SLG and SRK genes at the self-incompatibility locus of Brassica oleracea. Mol Gen Genet 236:369–374
  Google Scholar
• Devereux J, Haeberli P, Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387–395
  Google Scholar
• Draper J, Scott R (1988) Plant genetic transformation and gene expression. In: Scott R, Armitage P, Walden R (eds) The isolation of plant nucleic acids. Blackwell Scientific Publications, Oxford, pp 205–206
  Google Scholar
• Dretzen G, Bellard M, Sassone-Gorse P, Chambon P (1981) A reliable method for the recovery of DNA fragments from agarose and acrylamide gels. Anal Biochem 112:295–298
  Google Scholar
• Dwyer KG, Chao A, Cheng B, Chen C-H, Nasrallah JB (1989) The Brassica self-incompatibility multigene family. Genome 31:969–972
  Google Scholar
• Goring DR, Rothstein SJ (1992) The _S_-locus receptor kinase gene in a self-incompatible Brassica napus line encodes a functional serine/threonine kinase. Plant Cell 4:1273–1281
  Google Scholar
• Hanks SK, Quinn AM, Hunter T (1988) The protein kinase family: conserved features and deduced phylogeny of the catalytic domains. Science 241:42–52
  Google Scholar
• Hodgson CP, Fisk RV (1987) Hybridization probe size control: optimized ‘oligolabelling’. Nucleic Acids Res 15:6295
  Google Scholar
• Knighton DR, Zheng J, Eyck LFT, Ashford VA, Xuong N, Taylor SS, Sowadski JM (1991) Crystal structure of the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase. Science 253:407–414
  Google Scholar
• Lalonde BA, Nasrallah ME, Dwyer KG, Chen C-H, Barlow B, Nasrallah JB (1989) A highly conserved Brassica gene with homology to the _S_-locus-specific glycoprotein structural gene. Plant Cell 1:249–258
  Google Scholar
• Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York
  Google Scholar
• Murphy GJP, Kavanagh T (1988) Speeding up the sequencing of double-stranded DNA. Nucleic Acids Res 16:5198
  Google Scholar
• Nasrallah JB, Kao T-H, Goldberg ML, Nasrallah ME (1985) A cDNA clone encoding an _S_-locus-specific glycoprotein from Brassica oleracea. Nature 318:263–267
  Google Scholar
• Nasrallah JB, Kao T-H, Chen C-H, Goldberg ML, Nasrallah ME (1987) Amino-acid sequence of glycoproteins encoded by three alleles of the S locus of Brassica oleracea. Nature 326:617–619
  Google Scholar
• Nasrallah ME (1974) Genetic control of quantitative variation in self-incompatibility proteins detected by immunodiffusion. Genetics 76:45–50
  Google Scholar
• Nasrallah ME (1989) The genetics of self-incompatibility reactions in Brassica and the effects of supressor genes. In: Lord E, Bernier G (eds) Plant Reproduction: from floral induction to pollination. American Society of Plant Physiologists Symposium Series, vol 1, Rockville Md, pp 146–155
• Nasrallah ME, Kandasamy MK, Nasrallah JB (1992) A genetically defined _trans_-acting locus regulates _S_-locus function in Brassica. Plant Journal 2:497–506
  Google Scholar
• Stein JC, Howlett B, Boyes DC, Nasrallah ME, Nasrallah JB (1991) Molecular cloning of a putative receptor kinase gene encoded at the self-incompatibility locus of Brassica oleracea. Proc Natl Acad Sci USA 88:8816–8820
  Google Scholar
• Tobias CM, Howlett B, Nasrallah JB (1992) An Arabidopsis thaliana gene with sequence similarity to the _S_-locus receptor kinase of Brassica oleracea. Plant Physiol 99:284–290
  Google Scholar
• Trick M, Flavell RB (1989) A homozygous S genotype of Brassica oleracea expresses two _S_-like genes. Mol Gen Genet 218:112–117
  Google Scholar
• Walker JC, Zhang R (1990) Relationship of a putative receptor protein kinase from maize to the _S_-locus glycoproteins of Brassica. Nature 345:743–746
  Google Scholar
• Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn HT, Mullis KB, Erlich HA (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
  Google Scholar

Download references

Author information

Authors and Affiliations

1. Cambridge Laboratory, AFRO Institute of Plant Science Research, John Innes Centre, NR4 7UJ, Norwich, UK
   Vinod Kumar & Martin Trick

Authors

1. Vinod Kumar
2. Martin Trick

Additional information

Communicated by D.M. Lonsdale

Rights and permissions

About this article

Cite this article

Kumar, V., Trick, M. Sequence complexity of the S receptor kinase gene family in Brassica.Molec. Gen. Genet. 241, 440–446 (1993). https://doi.org/10.1007/BF00284698

Download citation

• Received: 19 February 1993
• Accepted: 30 April 1993
• Issue date: November 1993
• DOI: https://doi.org/10.1007/BF00284698

Key words