Chromosome assignment of two cloned DNA probes hybridizing predominantly to human sex chromosomes (original) (raw)

Summary

In situ hybridization experiments were carried out with two clones, YACG 35 and 2.8, which had been selected from two genomic libraries strongly enriched for the human Y chromosome. Besides the human Y chromosome, both sequences strongly hybridized to the human X chromosome, with few minor binding sites on autosomes. In particular, on the X chromosome DNA from clone YACG 35 hybridized to the centromeric region and the distal part of the short arm (Xp2.2). On the Y chromosome, the sequence was assigned to one site situated in the border region between Yq1.1 and Yq1.2. DNA from clone 2.8 also hybridized to the centromeric region of the X and the distal part of the short arm (Xq2.2). On the Y, however, two binding sites were observed (Yp1.1 and Yq1.2). The findings indicate that sex chromosomal sequences may be localized in homologous regions (as suggested from meiotic pairing) but also at ectopic sites.

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Authors and Affiliations

1. Institut für Anthropologie und Humangenetik, Universität Heidelberg, Im Neuenheimer Feld 328, D-6900, Heidelberg, Federal Republic of Germany
   Gudrun A. Rappold, T. Cremer & W. Back
2. Institut für Angewandte Physik, Universität Heidelberg, Albert-Überle-Str. 3-5, D-6900, Heidelberg, Federal Republic of Germany
   C. Cremer
3. Institut für Physiologische Chemie, Universität München, Schillerstr. 42, D-8000, München 2, Federal Republic of Germany
   J. Bogenberger
4. MRC Mammalian Genome Unit, Department of Zoology, University of Edinburgh, West Mains Road, EH 93JT, GB-Edinburgh
   H. J. Cooke

Authors

1. Gudrun A. Rappold
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2. T. Cremer
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3. C. Cremer
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4. W. Back
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5. J. Bogenberger
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6. H. J. Cooke
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Rappold, G.A., Cremer, T., Cremer, C. et al. Chromosome assignment of two cloned DNA probes hybridizing predominantly to human sex chromosomes.Hum Genet 65, 257–261 (1984). https://doi.org/10.1007/BF00286513

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• Received: 21 July 1983
• Revised: 12 September 1983
• Issue Date: January 1984
• DOI: https://doi.org/10.1007/BF00286513

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