The identification of 18 nuclear genes required for the expression of the yeast mitochondrial gene encoding cytochrome c oxidase subunit 1 (original) (raw)
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The complex regulation of mitochondrial gene expression in yeast is crucial for understanding cellular respiration and energy production. This study successfully identified 18 nuclear genes that are essential for the processing of the mitochondrial COX1 mRNA, revealing that most mutations lead to defects in splicing rather than transcription or stability of the mRNA.
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Eighteen nuclear mutants of the yeast Saccharomyces cerevisiae, each disturbed in the biosynthesis of the mitochondrially encoded cytochrome c oxidase subunit 1 (cox 1) and each representing a distinct complementation group, have been examined to identify the level at which COX1 expression is affected. RNA blotting revealed that most have a defect in the processing of COX1 precursor-mRNA; only a few are defective in COX1 transcription and/or pre-mRNA stability. In most RNA-processing mutants, the absence of the COX1 messenger results from a defect in the splicing of one or more COX1 introns. In turn, this defect can be ascribed to a mutation in a nuclear gene which is either directly involved in splicing or else acts indirectly by impairing COX1 translation.
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Authors and Affiliations
- Section for Molecular Biology, Department of Molecular Cell Biology, University of Amsterdam, Kruislaan 318, NL-1098 SM, Amsterdam, The Netherlands
H. J. Pel & L. A. Grivell - Department of Biological Sciences, Columbia University, 10027, New York, NY, USA
A. Tzagoloff
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- H. J. Pel
- A. Tzagoloff
- L. A. Grivell
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Communicated by R. J. Schweyen
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Pel, H.J., Tzagoloff, A. & Grivell, L.A. The identification of 18 nuclear genes required for the expression of the yeast mitochondrial gene encoding cytochrome c oxidase subunit 1.Curr Genet 21, 139–146 (1992). https://doi.org/10.1007/BF00318473
- Received: 29 August 1991
- Issue date: February 1992
- DOI: https://doi.org/10.1007/BF00318473