One-step transformation of yeast in stationary phase (original) (raw)

Summary

A fast yeast-transformation technique has been developed by adding thio compounds to alkali-ion based protocols and incubating at 45°C. This procedure is especially recommended for cells from stationary phase at a density up to 2.5×108 cells/ml. It involves only one step for the preparation and transformation of competent cells within 30 min. The yield was more than 104 transformants/μg plasmid DNA. This protocol is easy to scale up for many DNA samples and is also applicable for yeast cells from different types of storages.

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References

• Brzobohatý B, Kováč L (1986) J Gen Microbiol 132:3089–3093
  Google Scholar
• Hinnen A, Hicks JB, Fink GR (1978) Proc Natl Acad Sci USA 75:1929–1933
  Google Scholar
• Ito H, Fukuda Y, Murata K, Kimura A (1983a) J Bacteriol 153:163–168
  Google Scholar
• Ito H, Murata K, Kimura A (1983b) Agric Biol Chem 47:1691–1692
  Google Scholar
• Ito H, Murata K, Kimura A (1984) Agric Biol Chem 48:341–347
  Google Scholar
• Jones E (1976) Genetics 85:23–30
  Google Scholar
• Kadohda D, Abe N, Matsui M, Hayashibe M (1976) Plant Cell Physiol 17:909–919
  Google Scholar
• Kitamura K (1982) Agric Biol Chem 46:2093–2099
  Google Scholar
• Klebe RJ, Harriss JV, Sharp JD, Douglas MG (1983) Gene 25:223–241
  Google Scholar
• Meilhoc E, Masson J-M, Teissié J (1990) Bio/Technology 8:233–227
  Google Scholar
• Pastor FIJ, Herrero E, Sentandreu R (1982) Arch Microbiol 22:249–258
  Google Scholar
• Sikorski RS, Hieter P (1989) Genetics 122:19–27
  Google Scholar
• Sterns T, Hong M, Bostein D (1990) Methods Enzymol 185:280–297
  Google Scholar
• Zlotnik H, Fernadez MP, Bower B, Cabib E (1984) J Bacteriol 159:1018–1026
  Google Scholar

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Authors and Affiliations

1. Graduate Institute of Botany, National Taiwan University, Taipei, Republic of China
   Dz-Chi Chen & Tsong-Teh Kuo
2. Institute of Botany, Academia Sinica, Taipei, Republic of China
   Tsong-Teh Kuo
3. Institute of Molecular Biology, Academia Sinica, Taipei, Republic of China
   Dz-Chi Chen, Bei-Chang Yang & Tsong-Teh Kuo

Authors

1. Dz-Chi Chen
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2. Bei-Chang Yang
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3. Tsong-Teh Kuo
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Communicated by A. Hinnen

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Chen, DC., Yang, BC. & Kuo, TT. One-step transformation of yeast in stationary phase.Curr Genet 21, 83–84 (1992). https://doi.org/10.1007/BF00318659

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• Received: 26 August 1991
• Issue Date: January 1992
• DOI: https://doi.org/10.1007/BF00318659

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