Isolation in vitro of naphthaleneacetic acid-tolerant mutants of Nicotiana tabacum, which are impaired in root morphogenesis (original) (raw)
Summary
Mutants resistant to the toxic effect of the auxin analogue naphthaleneacetic acid have been selected in vitro in mutagenized populations of haploid mesophyll protoplasts of Nicotiana tabacum. Among the regenerated clones obtained, two clones impaired in root morphogenesis were further characterized. The parental mutant clones isolated were found to be heterozygotes for the mutations conferring the inability to root. These mutations were transmissible to the progeny as single nuclear dominant traits and cosegregated with resistance to naphthaleneacetic acid at the cellular level. Apart from the inability to root and a slightly modified leaf morphology, no obvious modification of stem structure, apical dominance, and flower fertility was detected in the development of homozygotes obtained in the progeny of one of the parental mutant clones when they were grafted onto normal plants.
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Abbreviations
NAA:
1-Naphthaleneacetic acid
PE:
plating efficiency
p-cells:
protoplast-derived dividing cells
MNNG:
N-methyl-N'-nitro-N-nitrosoguanidine
UV:
Ultraviolet light
2,4-D:
2,4-dichlorophenoxyacetic acid
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Authors and Affiliations
- Laboratoire de biologie cellulaire, I.N.R.A., F-78000, Versailles, France
Jean-François Muller, Jacques Goujaud & Michel Caboche
Authors
- Jean-François Muller
- Jacques Goujaud
- Michel Caboche
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Dedicated to Professor Georg Melchers to celebrate his 50-year association with the journal
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Muller, JF., Goujaud, J. & Caboche, M. Isolation in vitro of naphthaleneacetic acid-tolerant mutants of Nicotiana tabacum, which are impaired in root morphogenesis.Molec Gen Genet 199, 194–200 (1985). https://doi.org/10.1007/BF00330259
- Received: 10 September 1984
- Issue date: May 1985
- DOI: https://doi.org/10.1007/BF00330259