Operon structure of flagellar genes in Salmonella typhimurium (original) (raw)

Summary

In Salmonella typhimurium, more than 40 genes have been shown to be involved in flagellar formation and function and almost all of them have been assigned to three regions of the chromosome, termed region I, region II, and region III. In the present study, a large number of transposon-insertion mutants in these flagellar genes were isolated using Tn_10_ and Mu_d1_. The flaV gene was found to be a strong hot spot for Tn_10_ insertion. Complementation analysis of the polarity effects exerted by the transposon-insertion mutants defined 13 different flagellar operons; 3 in region I, 4 in region II, and 6 in region III. These results are compared with the reported arrangement of the corresponding genes in Escherichia coli.

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References

• Blazey DL, Burns RO (1982) Transcriptional activity of the transposable element Tn_10_ in the Salmonella typhimurium ilvGEDA operon. Proc Natl Acad Sci USA 79:5011–5015
  Google Scholar
• Casadaban MJ, Cohen SN (1979) Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: In vivo probe for transcriptional control sequences. Proc Natl Acad Sci USA 76:4530–4533
  Google Scholar
• Ciampi MS, Schmid MB, Roth JR (1982) Transposon Tn_10_ provides a promoter for transcription of adjacent sequences. Proc Natl Acad Sci USA 79:5016–5020
  Google Scholar
• Csonka LN, Howe MM, Ingraham JL, Pierson III LS, Turnbough Jr CL (1981) Infection of Salmonella typhimurium with coliphage Mu_d1_ (Apr lac): Construction of pyr::lac gene fusions. J Bacteriol 145:299–305
  Google Scholar
• Davis RW, Botstein D, Roth JR (1980) Quick method for the preparation of P22 transducing phage. In: Davis RW, Botstein D, Roth JR (eds) Advanced bacterial genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp 78–79
  Google Scholar
• DeFranco AL, Koshland Jr DE (1981) Molecular cloning of chemotaxis genes and overproduction of gene products in the bacterial sensing system. J Bacteriol 147:390–400
  Google Scholar
• DeFranco AL, Parkinson JS, Koshland Jr DE (1979) Functional homology of chemotaxis genes in Escherichia coli and Salmonella typhimurium. J Bacteriol 139:107–114
  Google Scholar
• Fujita H, Yamaguchi S, Iino T (1973) Studies on H-O variants in Salmonella in relation to phase variation. J Gen Microbiol 76:127–134
  Google Scholar
• Halling SM, Kleckner N (1982) A symmetrical six-base-pair target site sequence determines Tn_10_ insertion specificity. Cell 28:155–163
  Google Scholar
• Homma M, Iino T, Macnab RM (1988) Identification and characterization of the products of six region III flagellar genes (flaAII.3 through flaQII) of Salmonella typhimurium. J Bacteriol 170:2221–2228
  Google Scholar
• Kleckner N, Roth J, Botstein D (1977) Genetic engineering in vivo using transposable drug-resistance elements. J Mol Biol 116:125–159
  Google Scholar
• Komeda Y (1982) Fusions of flagellar operons to lactose genes on a Mu lac bacteriophage. J Bacteriol 150:16–26
  Google Scholar
• Komeda Y, Kutsukake K, Iino T (1980) Definition of additional flagellar genes in Escherichia coli K12. Genetics 94:277–290
  Google Scholar
• Konno R, Fujita H, Horiguchi T, Yamaguchi S (1976) Precise position of the nml locus on the genetic map of Salmonella. J Gen Microbiol 93:182–184
  Google Scholar
• Kutsukake K, Iino T (1985) Refined genetic analysis of the region II che mutants in Salmonella typhimurium. Mol Gen Genet 199:406–409
  Google Scholar
• Kutsukake K, Suzuki T, Yamaguchi S, Iino T (1979) Role of gene flaFV on flagellar hook formation in Salmonella typhimurium. J Bacteriol 140:267–275
  Google Scholar
• Kutsukake K, Iino T, Komeda Y, Yamaguchi S (1980) Functional homology of fla genes between Salmonella typhimurium and Escherichia coli. Mol Gen Genet 178:59–67
  Google Scholar
• Lee SY, Butler D, Fleckner N (1987) Efficient Tn_10_ transposition into a DNA insertion hot spot in vivo requires the 5-methyl groups of symmetrically disposed thymines within the hot-spot consensus sequence. Proc Natl Acad Sci USA 84:7876–7880
  Google Scholar
• Macnab RM (1987) Flagella. In: Neidhardt FC (ed) Escherichia coli and Salmonella typhimurium. American Society for Microbiology, Washington, DC, pp 70–83
  Google Scholar
• Ornellas EP, Stocker BAD (1974) Relation of lipopolysaccharide character to P1 sensitivity in Salmonella typhimurium. Virology 60:491–502
  Google Scholar
• Russo AF, Koshland Jr DE (1986) Identification of the _tip_-encoded receptor in bacterial sensing. J Bacteriol 165:276–282
  Google Scholar
• Schmieger H (1972) Phage P22 mutants with increased or decreased transduction abilities. Mol Gen Genet 119:75–88
  Google Scholar
• Silverman M, Simon M (1973) Genetic analysis of bacteriophage Mu-induced flagellar mutants in Escherichia coli. J Bacteriol 116:114–122
  Google Scholar
• Simons RW, Hoopes BC, McClure WR, Kleckner N (1983) Three promoters near the termini of IS_10_: pIN, pOUT, and pIII. Cell 34:673–682
  Google Scholar
• Slocum MK, Parkinson JS (1983) Genetics of methyl-accepting chemotaxis proteins in Escherichia coli: Organization of the tar region. J Bacteriol 155:565–577
  Google Scholar
• Suzuki T, Komeda Y (1981) Incomplete flagellar structures in Escherichia coli mutants. J Bacteriol 145:1036–1041
  Google Scholar
• Yamaguchi S, Fujita H, Taira T, Kutsukake K, Homma M, Iino T (1984) Genetic analysis of three additional fla genes in Salmonella typhimurium. J Gen Microbiol 130:3339–3342
  Google Scholar
• Yamaguchi S, Fujita H, Ishihara A, Aizawa S, Macnab RM (1986a) Subdivision of flagellar genes of Salmonella typhimurium into regions responsible for assembly, rotation, and switching. J Bacteriol 166:187–193
  Google Scholar
• Yamaguchi S, Aizawa S, Kihara M, Isomura M, Jones CJ, Macnab RM (1986b) Genetic evidence for a switching and energy-transducing complex in the flagellar motor of Salmonella typhimurium. J Bacteriol 168:1172–1179
  Google Scholar

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Authors and Affiliations

1. Laboratory of Genetics, Department of Biology, Faculty of Science, University of Tokyo, Hongo, 113, Tokyo, Japan
   Kazuhiro Kutsukake, Yoshikazu Ohya & Tetsuo Iino
2. Department of Biology, School of Education, Waseda University, Nishiwaseda, Shinjuku-ku, 160, Tokyo, Japan
   Shigeru Yamaguchi

Authors

1. Kazuhiro Kutsukake
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2. Yoshikazu Ohya
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3. Shigeru Yamaguchi
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4. Tetsuo Iino
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Communicated by K. Isono

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Kutsukake, K., Ohya, Y., Yamaguchi, S. et al. Operon structure of flagellar genes in Salmonella typhimurium.Mol Gen Genet 214, 11–15 (1988). https://doi.org/10.1007/BF00340172

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• Received: 20 February 1988
• Issue Date: September 1988
• DOI: https://doi.org/10.1007/BF00340172

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