Core-Strukturen in den meiotischen und post-meiotischen Kernen der Spermatogenese von Gryllus domesticus (original) (raw)

Summary

electron microscope study of spermatogenesis and spermiogenesis in Gryllus domesticus has revealed the existence of peculiar lamellate bodies which occur both in spermatocyte and spermatid nuclei. These bodies must be considered as multiple complexes of the axial core structures which are regularly found in paired pachytene chromosomes. Their shape is irregular, and their constituent structural elements, although having dimensions and a fine structure identical to those of regular axial complexes, may assemble in sheets rather than ribbons, often in a concentric rather than planparallel multiple layer system.

Spcrmatocyte nuclei may either contain just one or two large bodies of this type, often but not always in close association with the nucleolus and/or the X chromosome, or they may show several such structures of smaller dimensions which have some connection to chromosome fibrils. None of the two types of nuclei simultaneously contains regular axial core complexes. In spermatid nuclei one or two such multiple structures are usually found, again often in association to the X and/or (in O-spermatids) to what appears to be a nucleolus.

It is considered likely that the multiple core complexes are due to the self-assembly of those protein molecules which typically assemble only under the control of and in close association with the pachytene chromosomes to form ordered axial complexes. Their occurrence in spermatids shows that the constituent molecular material may not be decomposed during the meiotic divisions after it is dissociated from the chromosomes.

Similar content being viewed by others

Literatur

• Coleman, J. R., and M. J. Moses: DNA and the fine structure of synaptic chromosomes in the domestic rooster (Gallus domesticus). J. Cell Biol. 23, 63–78 (1964).
  Google Scholar
• Fawcett, D. W.: The fine structure of chromosomes in the meiotic prophase of vertebrate spermatocytes. J. biophys. biochem. Cytol. 2, 403–406 (1956).
  Google Scholar
• Franchi, L. L., and A. M. Mandl: The ultrastructure of oogonia and oocytes in the foetal and neonatal rat. Proc. roy. Soc. B 157, 99–114 (1962).
  Google Scholar
• Meyer, G. F.: The fine structure of spermatocyte nuclei of Drosophila melanogaster. Proc. Eur. Reg. Conf. on Electron Microscopy (Delft) 1960, p. 951–954; - A possible correlation between the submicroscopic structure of meiotic chromosomes and crossing over. Proc. Eur. Reg. Conf. on Electron Microscopy (Prague) 1964, p. 461–462.
• Moses, M. J.: Studies on nuclei using correlated cytochemical, light, and electron microscope techniques. J. biophys. biochem. Cytol. 2 (Suppl.), 397–406 (1956a); - Chromosomal structures in crayfish spermatocytes. J. biophys. biochem. Cytol. 2, 215–218 (1956b); - The relation between the axial complex of meiotic prophase chromosomes and chromosome pairing in a salamander (Plethoden cinereus). J. biophys. biochem. Cytol. 4, 633–638 (1958a); - Patterns of organization in the fine structure of chromosomes. IV. Int. Kongr. Elektronenmikroskopie (Berlin) 2, 199–211 (1958b).
  Google Scholar
• Nebel, B. R., and E. M. Coulon: The fine structure of chromosomes in pigeon spermatocytes. Chromosoma (Berl.) 13, 272–291 (1962).
  Google Scholar
• Schin, K. S.: Meiotische Prophase und Spermatidenreifung bei Gryllus domesticus, mit besonderer Berücksichtigung der Chromosomenstruktur. Z. Zellforsch. 65, 481–513 (1965).
  Google Scholar
• Sotelo, J. R., and O. Trujillo-Cenóz: Submicroscopic structure of meiotic chromosomes during prophase. Exp. Cell Res. 14, 1–8 (1958); - Electron microscope study on spermatogenesis, chromosome morphogenesis at the onset of meiosis (cyte I) and nuclear structure of early and late spermatids. Z. Zellforsch. 51, 243–277 (1960).
  Google Scholar
• —, and R. Wettstein: Electronmicroscope study on meiosis. The sex chromosome in spermatocytes, spermatids, and oocytes of Gryllus argentinus. Chromosoma (Berl.) 15, 389–415 (1964).
  Google Scholar

Download references

Author information

Authors and Affiliations

1. Abt. Beermann, I. Zoologischen Institut der Universität Göttingen, Tübingen
   Ki Ssu Schin
2. Abt. Beermann, Max-Planck-Institut für Biologie, Tübingen
   Ki Ssu Schin
3. Department of Biologial Sciences, Purdue University Lafayette, Indiana, USA
   Ki Ssu Schin

Additional information

Mit Unterstützung durch die Göttinger Akademie der Wissenschaften.

Herrn Prof. Dr. H. Bauer zu seinem 60. Geburtstag gewidmet.

Rights and permissions

About this article

Cite this article

Schin, K.S. Core-Strukturen in den meiotischen und post-meiotischen Kernen der Spermatogenese von Gryllus domesticus.Chromosoma 16, 436–452 (1965). https://doi.org/10.1007/BF00343172

Download citation

• Received: 27 September 1964
• Issue date: July 1965
• DOI: https://doi.org/10.1007/BF00343172