Homologies in human and Macasa fuscata chromosomes revealed by in situ suppression hybridization with human chromosome specific DNA libraries (original) (raw)

Abstract

We established chromosomal homologies between all chromosomes of the human karyotype and that of an old world monkey (Macaca fuscata) by chromosomal in situ suppression (CISS) hybridization with human chromosome specific DNA libraries. Except for the human chromosome 2 library and limited cross-hybridization of X and Y chromosome libraries all human DNA libraries hybridized to single GTG-banded macaque chromosomes. Only three macaque chromosomes (2, 7, 13) were each hybridized by two separate human libraries (7 and 21, 14 and 15, 20 and 22 respectively). Thus, an unequivocally high degree of synteny between human and macaque chromosomes has been maintained for more than 20 million years. As previously suggested, both Papionini (macaques, baboons, mandrills and cercocebus monkeys, all of which have nearly identical karyotypes) and humans are chromosomally conservative. The results suggest, that CISS hybridization can be expected to become an indispensable tool in comparative chromosome and gene mapping and will help clarify chromosomal phylogenies with speed and accuracy.

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1. J. Wienberg
   Present address: Institut für Anthropologie und Humangenetik, Richard-Wagner-Strasse 10, W-8000, München, Federal Republic of Germany

Authors and Affiliations

1. Institut für Humangenetik und Anthropologie, Im Neuenheimer Feld 328, W-6900, Heidelberg, Federal Republic of Germany
   J. Wienberg, A. Jauch & T. Cremer
2. Istituto di Antropologia, Universita Genoa, Via Balbi 4, 1, Genoa, Italy
   R. Stanyon

Authors

1. J. Wienberg
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2. R. Stanyon
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3. A. Jauch
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4. T. Cremer
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by E.R. Schmidt

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Wienberg, J., Stanyon, R., Jauch, A. et al. Homologies in human and Macasa fuscata chromosomes revealed by in situ suppression hybridization with human chromosome specific DNA libraries.Chromosoma 101, 265–270 (1992). https://doi.org/10.1007/BF00346004

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• Received: 14 August 1991
• Accepted: 12 September 1991
• Issue Date: March 1992
• DOI: https://doi.org/10.1007/BF00346004

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