Polymorphism for PCR-analyzed microsatellites between the inbred mouse strains LG and SM (original) (raw)

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Abstract

Using agarose gel electrophoresis, we surveyed four strains of inbred mice (AKR/J, C57BL/J, LG/J, and SM/J) for 472 microsatellite loci. Agarose electrophoresis proved to be extremely efficient in separating alleles differing by six or more base pairs and detected a majority of allelic differences of between two and six base pairs. Overall, 64.4% of loci showed polymorphism among the four strains, and pairwise comparisons ranged from 42.1% to 48.1%. Microsatellite polymorphism for strains LG/J and SM/J has not been previously described and was sufficiently high (47.1%) to make these size-divergent strains excellent candidates for quantitative trait loci (QTL) analysis of normal growth.

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Author notes

1. E. J. Routman
   Present address: Department of Biology, San Francisco State University, 1600 Holloway Avenue, 94132, San Francisco, CA, USA

Authors and Affiliations

1. Department of Anatomy and Neurobiology, Washington University School of Medicine, 63110, St. Louis, Missouri, USA
   E. J. Routman & J. M. Cheverud

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1. E. J. Routman
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2. J. M. Cheverud
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Routman, E.J., Cheverud, J.M. Polymorphism for PCR-analyzed microsatellites between the inbred mouse strains LG and SM.Mammalian Genome 6, 401–404 (1995). https://doi.org/10.1007/BF00355640

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• Received: 10 October 1994
• Accepted: 24 February 1995
• Issue Date: June 1995
• DOI: https://doi.org/10.1007/BF00355640

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