Industrial production of monoclonal antibodies and therapeutic proteins by dialysis fermentation (original) (raw)

Abstract

A novel and powerful fermentation method is reported for the large-scale growth of mammalian cells and their secreted products. The system described illustrates many of the advantages of conventional batch fermentation processes but in addition has been shown to yield cell densities in excess of 1×107 cells/ml with concomitant increase in product concentration.

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Abbreviations

MAb:

Monoclonal Antibody

STR:

Stirred Tank Reactor

FBS:

Foetal Bovine Serum

References

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Authors and Affiliations

1. Research Centre Penzberg, Boehringer Mannheim GmbH, Nonnenwald, 8122, Penzberg, Germany
   Michael J. Comer, Michael J. Kearns, Jürgen Wahl, Michael Munster, Thomas Lorenz, Berthold Szperalski, Stefan Koch, Ulrich Behrendt & Herwig Brunner

Authors

1. Michael J. Comer
2. Michael J. Kearns
3. Jürgen Wahl
4. Michael Munster
5. Thomas Lorenz
6. Berthold Szperalski
7. Stefan Koch
8. Ulrich Behrendt
9. Herwig Brunner

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Comer, M.J., Kearns, M.J., Wahl, J. et al. Industrial production of monoclonal antibodies and therapeutic proteins by dialysis fermentation.Cytotechnology 3, 295–299 (1990). https://doi.org/10.1007/BF00365493

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• Received: 14 August 1989
• Accepted: 09 November 1989
• Issue date: May 1990
• DOI: https://doi.org/10.1007/BF00365493

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