Islet amyloid polypeptide amide causes peripheral insulin resistance in vivo in dogs (original) (raw)

Summary

Islet amyloid polypeptide is a 37 amino acid hormone-like peptide which is the major protein component of islet amyloid deposits commonly found in patients with Type 2 (non-insulin-dependent) diabetes mellitus. Recent studies indicate that a physiologically active form of this peptide appears to be carboxyamidated and secreted from the insulin-producing beta cell. In order to clarify the possible in vivo actions of islet amyloid polypeptide, we have studied the effects of synthesized islet amyloid polypeptide-amide on peripheral glucose utilization by performing hyperinsulinaemic euglycaemic glucose clamp studies on dogs. Exogenously administered islet amyloid polypeptide-amide (an infusion from 1.0 to 100 μg·kg−1·h−1, over 2 h) inhibited the insulin-stimulated glucose disposal rate in a dose dependent manner. Twenty-five μg·kg−1·h−1 of islet amyloid polypeptide-amide infused via a peripheral vein significantly lowered the glucose disposal rate by 20% (from 17.4±1.7 to 14.4±1.7 mg·kg−1·min−1, n = 5, p<0.01). These findings suggest that islet amyloid polypeptide-amide causes peripheral insulin resistance in vivo.

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Authors and Affiliations

  1. First Department of Medicine, Wakayama University of Medical Science, Wakayama, Japan
    R. Sowa, T. Sanke, J. Hirayama, H. Tabata, H. Furuta, S. Nishimura & K. Nanjo

Authors

  1. R. Sowa
  2. T. Sanke
  3. J. Hirayama
  4. H. Tabata
  5. H. Furuta
  6. S. Nishimura
  7. K. Nanjo

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Sowa, R., Sanke, T., Hirayama, J. et al. Islet amyloid polypeptide amide causes peripheral insulin resistance in vivo in dogs.Diabetologia 33, 118–120 (1990). https://doi.org/10.1007/BF00401051

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