Mapping of rRNA genes by integration of hybrid plasmids in Schizosaccharomyces pombe (original) (raw)
Summary
The major rRNA genes of the fission yeast Schizosaccharomyces pombe were mapped on chromosome III by plasmid integration. The integration vector YIp33 containing S. cerevisiae LEU2 gene was combined with the S. pombe rDNA. Since LEU2 complements S. pombe leu1 deficiency, it could be used as the genetic marker for integration. The 10.4 kb rDNA repeat contained ARS sequence, and therefore 2.4 kb and 0.7 kb subfragments not containing ARS were subcloned into YIp33 and transformed leu1 S. pombe cells to Leu+. Genetic analyses of the transformants indicated that the integrated rDNA resides in the long arm of the shortest chromosome III, tightly linked to ade5 (1.4 cM). This result is consistent with our previous finding that the DAPI-stained smallest chromosomes were associated with the nucleolus (Umesono et al. 1983).
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Abbreviations
ARS:
autonomously replicating sequence
DAPI:
4′,6-diamidino-2-phenylindole
kb:
kilo base pairs
rDNA:
DNA segment containing ribosomal RNA genes
rRNA:
ribosomal RNA
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Authors and Affiliations
- Department of Biophysics, Faculty of Science, Kyoto University, 606, Sakyo-ku, Kyoto, Japan
Takashi Toda, Yukinobu Nakaseko, Osami Niwa & Mitsuhiro Yanagida
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- Takashi Toda
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Toda, T., Nakaseko, Y., Niwa, O. et al. Mapping of rRNA genes by integration of hybrid plasmids in Schizosaccharomyces pombe.Curr Genet 8, 93–97 (1984). https://doi.org/10.1007/BF00420224
- Received: 09 November 1983
- Published: 01 February 1984
- Issue Date: February 1984
- DOI: https://doi.org/10.1007/BF00420224