Complementation experiments between conditional lethal mutants of bacteriophage ϕX174 (original) (raw)
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Summary
Complementation experiments with temperature sensitive (ts) and suppressor sensitive (sus) mutants of bacteriophage ϕX174 unambiguously revealed five cistrons on the basis of a clear bipartition of burst sizes.
A new group of sus mutants (emeralds) was found, defective in a function essential for growth in Shigella sonnei V64.
The complementation between ts and sus mutants was in general asymmetric in that the yield of ts particles was lower than that of the sus particles. The mutants of one cistron (defective in RF-replication) showed a completely asymmetric complementation behaviour both of ts and sus mutants. The ts mutants of this group, which show to be early, appear to be defective in two functions.
The possibility is discussed that in each cell only one phage genome is replicated. This would explain both kinds of asymmetric complementation and the low burst sizes that were obtained when mutants of particular genes were complemented.
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References
- Adams, M. H.: Methods of study of bacterial viruses. In: Bacteriophages. New York: Interscience Publishers 1959.
Google Scholar - Baker, R., and I. Tessman: Heat stability of mutants in genes II, IIIa and VI of phage S13. Virology 35, 179–181 (1968).
Google Scholar - Dowell, C. E., and R. L. Sinsheimer: The process of infection with bacteriophage ϕX174. IX. Studies on the physiology of three temperature-sensitive mutants. J. molec. Biol. 16, 374–386 (1966).
Google Scholar - Fraser, D., and E. A. Jerrel: The amino acid composition of the bacteriophage T3. J. biol. Chem. 205, 291 (1953).
Google Scholar - Hutchison, C. A., and R. L. Sinsheimer: The process of infection with bacteriophage ϕX174. X. Mutations in the ϕX-lysis gene. J. molec. Biol. 18, 429–447 (1966).
Google Scholar - Jansz, H. S., C. van Rotterdam, and J. A. Cohen: Genetic recombination of bacteriophage ϕX174 in spheroplasts. Biochim. biophys. Acta (Amst.) 119, 276–288 (1966).
Google Scholar - Knippers, R., and R. L. Sinsheimer: The process of infection with bacteriophage ϕX174. XXIII. DNA synthesis in cells infected by a coat protein mutant. J. molec. Biol. 35, 591–605 (1968).
Google Scholar - Lindqvist, B. H., and R. L. Sinsheimer: The process of infection with bacteriophage ϕX174. XIV. Studies on macromolecular synthesis during infection with a lysis-defective mutant. J. molec. Biol. 28, 87–94 (1967a).
Google Scholar - ——: The process of infection with bacteriophage ϕX174. XV. Bacteriophage DNA synthesis in abortive infections with a set of conditional lethal mutants. J. molec. Biol. 30, 69–80 (1967b).
Google Scholar - Markert, A., and W. Zillig: Studies on the lysis of Escherichia coli C by bacteriophage ϕX174. Virology 25, 88–97 (1965).
Google Scholar - Poljak, R. J.: A study of the coat proteins of bacteriophage ϕX174. Virology 35, 185–193 (1968).
Google Scholar - Salivar, W. O., and R. L. Sinsheimer: Intracellular location and number of replicating parental DNA molecules of bacteriophages lambda and ϕX174. J. molec. Biol. 41, 39–65 (1969).
Google Scholar - Siegel, J. E. D., M. N. Hayashi, and M. Hayashi: ϕX174 coat protein mutants affecting DNA synthesis. Biochem. biophys. Res. Commun. 31, 774–778 (1968).
Google Scholar - Sinsheimer, R. L.: Purification and properties of bacteriophage ϕX174. J. molec. Biol. 1, 37–42 (1959).
Google Scholar - —: Bacteriophage ϕX174 and related viruses. Progr. Nucl. Acid Res. and molec. Biol. 8, 115–169 (1968).
Google Scholar - —, C. A. Hutchison, and B. H. Lindqvist: Bacteriophage ϕX174: Viral functions. In: The molecular biology of viruses (edit. J. S. Colter and W. Paranchych), p. 175–192. New York: Academic Press 1967.
Google Scholar - Tessman, E. S.: Complementation groups in phage S13. Virology 25, 303–321 (1965).
Google Scholar - —: Mutants of bacteriophage S13 blocked in infectious DNA synthesis. J. molec. Biol. 17, 218–236 (1966).
Google Scholar - —: Gene function in phage S13. In: The molecular biology of viruses (edit. J. S. Colter and W. Paranchych), p. 193–209. New York: Academic Press 1967.
Google Scholar - Tessman, I., H. Ishiwa, S. Kumar, and R. Baker: Bacteriophage S13: A seventh gene. Science 156, 824–825 (1967).
Google Scholar - Thomas, R., C. Leurs, C. Dambly, D. Parmentier, L. Lambert, P. Brachet, N. Lefebvre, S. Mousset, J. Porcheret, J. Spzirer, and D. Wauters: Isolation and characterization of new sus (amber) mutants of bacteriophage λ. Mutation Res. 4, 735–741 (1967).
Google Scholar - Van de Pol, J. H.: Mechanism of mutation induction and inactivation by hydroxylamine. [Dutch.] Thesis Univ. of Utrecht (1966).
- Van de Vate, C., E. H. Ten Bensel-Burger, and K. W. van de Poll: Suppression in bacteriophage ϕX174. Genetica 38, 404–405 (1968).
Google Scholar - Zahler, S. A.: Some biological properties of bacteriophages S13 and ϕX174. J. Bact. 75, 310–315 (1958).
Google Scholar
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Authors and Affiliations
- Institute of Genetics, State University, Utrecht, The Netherlands
Wilhelmina E. Borrias, J. H. van de Pol, C. van de Vate & G. A. van Arkel
Authors
- Wilhelmina E. Borrias
- J. H. van de Pol
- C. van de Vate
- G. A. van Arkel
Additional information
Communicated by W. Arber
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Borrias, W.E., van de Pol, J.H., van de Vate, C. et al. Complementation experiments between conditional lethal mutants of bacteriophage ϕX174.Molec. Gen. Genet. 105, 152–163 (1969). https://doi.org/10.1007/BF00445685
- Received: 10 January 1969
- Issue date: June 1969
- DOI: https://doi.org/10.1007/BF00445685