Genetics of an esterase in Aedes aegypti (original) (raw)
Abstract
Zymograms of single individuals of Aedes aegypti were obtained by means of starch gel electrophoresis, using alpha-naphthyl acetate as substrate. Inbred lines gave consistently homogeneous patterns; earlier results from random-breeding laboratory strains had shown considerable variability. Six distinct bands were observed. The furthest moving band, designated Esterase 6, showed differential migration in two inbred lines. Reciprocal crosses between these lines gave F1 progeny showing both bands. Backcrosses of F1 to either parental line gave a 1:1 segregation. These results are consistent with the hypothesis that the two forms of Esterase 6 are controlled by a single pair of codominant alleles at a single gene locus (Est 6 a and Est 6 b). Linkage tests with marker genes have demonstrated that Est 6 is on linkage group 2, with the following alignment: spot-abdomen (9.0±1.0) yellow-larva (17.4±1.3) Est 6. Crosses with another inbred line demonstrated a third band with intermediate mobility, designated Est 6 c. An additional electrophoretic variant which seems to have a simple Mendelian basis was found in esterase band 1.
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Author notes
- Alice M. Trebatoski
Present address: Department of Biology, Pace College, New York, New York
Authors and Affiliations
- Department of Biology, University of Notre Dame, Notre Dame, Indiana
Alice M. Trebatoski & George B. Craig Jr.
Authors
- Alice M. Trebatoski
- George B. Craig Jr.
Additional information
This work was supported by NIH Research Grant No. A1-02753.
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Trebatoski, A.M., Craig, G.B. Genetics of an esterase in Aedes aegypti.Biochem Genet 3, 383–392 (1969). https://doi.org/10.1007/BF00485722
- Received: 16 December 1968
- Revised: 16 March 1969
- Issue date: August 1969
- DOI: https://doi.org/10.1007/BF00485722