Thin-layer chromatography of radioactively labelled cholesterol and precursors from biological material (original) (raw)

Summary

The investigation methods of the action of xenobiotics on sterol biosynthesis from 14C-acetate in rat hepatocyte cultures can be developed, with regard to extraction using Extrelut and the separation of the sterol pattern by thin-layer chromatography, in such a way that they are suitable for wider application, e.g., screening. Good visualisation and recognition of changes in the sterol pattern are possible using autoradiography of the thin-layer chromatogram.

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Authors and Affiliations

1. Departments of Medical and Chemical Research, Boehringer Mannheim GmbH, Sandhofer Strasse 116, D-6800, Mannheim, Federal Republic of Germany
   J. Pill, J. Aufenanger, K. Stegmeier, F. H. Schmidt & D. Müller

Authors

1. J. Pill
2. J. Aufenanger
3. K. Stegmeier
4. F. H. Schmidt
5. D. Müller

Additional information

Dedicated to Dr. E. Roesch on occasion of his 65th birthday

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Pill, J., Aufenanger, J., Stegmeier, K. et al. Thin-layer chromatography of radioactively labelled cholesterol and precursors from biological material.Z. Anal. Chem. 327, 558–560 (1987). https://doi.org/10.1007/BF00487248

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• Received: 19 September 1986
• Issue date: January 1987
• DOI: https://doi.org/10.1007/BF00487248

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