The biochemical genetics of permethrin resistance in the Learn-PyR strain of house fly (original) (raw)
Abstract
Permethrin resistance in the Learn-PyR strain of house fly was examined in four genetically derived substrains, each being homozygous for a different “resistant” autosome of the Learn-PyR strain. The resistance of these derivative strains was characterized toxicologically and biochemically. The relative levels of resistance to permethrin conferred by each autosome were 5>3>1>2. Three factors were associated with resistance: (1) increased mixed-function oxidase (MFO) activity associated with elevated levels of cytochrome P-450, cytochrome b5, and NADPH-cytochrome c reductase (P-450 reductase) activity; (2) target-site insensitivity (kdr); and (3) decreased cuticular penetration. Permethrin resistance factors on chromosome 1 consisted of a piperonyl butoxide (PB)-suppressible mechanism correlated with increased levels of cytochromes P-450 and b5; on chromosome 2, a PB-suppressible mechanism associated with elevated amounts of cytochrome P-450; on chromosome 3, decreased cuticular penetration, kdr, and increased amounts of P-450 reductase activity; and on chromosome 5, a largely PB-suppressible mechanism correlated with elevated levels of cytochrome P-450 and P-450 reductase activity.
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References
- Adams, M. E., and Miller, T. A. (1979). Site of action of pyrethroids: Repetitive “backfiring” in flight motor units of house fly. Pestic. Biochem. Physiol. 11218.
Google Scholar - Anonymous (1984). Pyrethroid sales. Calif. Ariz. Press **23:**2.
- Farnham, A. W. (1973). Genetics of resistance of pyrethroid-selected houseflies, Musca domestica L. Pest. Sci. 4513.
Google Scholar - Fine, B. C., Goodin, P. J., and Thain, E. M. (1963). Penetration of pyrethrin I labelled with carbon-14 into susceptible and pyrethroid resistant houseflies. Nature 199927.
Google Scholar - Finney, D. J. (1952). Probit Analysis 2nd ed., Cambridge University Press, Cambridge.
Google Scholar - Georghiou, G. P. (1969). Genetics of resistance to insecticides in houseflies and mosquitoes. Exp. Parisitol. 26224.
Google Scholar - Hodgson, E. (1983). The significance of cytochrome P-450 in insects. Insect Biochem. 13237.
Google Scholar - MacDonald, R. S., Solomon, K. R., Surgeoner, G. A., and Harris, C. R. (1985). Laboratory studies on the mechanisms of resistance to permethrin in a field-selected strain of house flies. Pest. Sci. 1610.
Google Scholar - Motoyama, N. (1984). Pyrethroid resistance in a Japanese colony of the housefly. J. Pest. Sci. 9523.
Google Scholar - Murray, D., and Cull, P. (1984). Resistance strategy works at Emerald. Austral. Cottongrower Feb.–Apr.:22.
- Omura, T., and Sato, R. (1964). The carbon monoxide-binding pigment of liver microsomes. J. Biol. Chem. 2392370.
Google Scholar - Plapp, F. W., Jr. (1984). The genetic basis of insecticide resistance in the house fly: Evidence that a single locus plays a major role in metabolic resistance to insecticides. Pest. Biochem. Physiol. 22194.
Google Scholar - Plapp, F. W., Jr., and Casida, J. E. (1969). Genetic control of house fly NADPH-dependent oxidases: Relation to insecticide chemical metabolism and resistance. J. Econ. Entomol. 621174.
Google Scholar - Plapp, F. W., Jr., and Hoyer, R. F. (1968). Possible pleiotropism of a gene conferring resistance to DDT, DDT analogs, and pyrethrins in the house fly and Culex tarsalis. J. Econ. Entomol. 61761.
Google Scholar - Sawicki, R. M., and Farnham, A. W. (1967). The use of visible mutant markers in the study of resistance of house flies to insecticides. Proc. Fourth Brit. Insect. Fung. Conf. 1355.
Google Scholar - Sawicki, R. M., Devonshire, A. L., Farnham, A. W., O'Dell, K., and Morres, G. D. (1984). Factors affecting resistance to insecticides in house-flies, Musca domestica L. II. Close linkage on autosome 2 between an esterase and resistance to trichlorphon and pyrethroids. Bull. Entomol. Res. 74197.
Google Scholar - Scott, J. G., and Georghiou, G. P. (1984). Influence of temperature on knockdown, toxicity, and resistance to pyrethroids in the house fly, Musca domestica. Pest. Biochem. Physiol. 2153.
Google Scholar - Scott, J. G., and Georghiou, G. P. (1985a). Rapid development of high-level permethrin resistance in a field collected strain of house fly under laboratory selection. J. Econ. Entomol. 78 316.
Google Scholar - Scott, J. G., and Georghiou, G. P. (1985b). Mechanisms responsible for high levels of permethrin resistance in the house fly. Pest. Sci. (in press).
- Scott, J. G., Shono, T., and Georghiou, G. P. (1984). Genetic analysis of permethrin resistance in the house fly, Musca domestica L. Experientia 40416.
Google Scholar - Tsukamoto, M. (1983). Methods of genetic analysis of insecticide resistance. In Georghiou, G. P., and Saito, T. (eds.), Pest Resistance to Pesticides Plenum Press, New York, p. 71.
Google Scholar - Yu, S. J., and Terriere, L. C. (1979). Cytochrome P-450 in insects. 1. Differences in the forms present in insecticide resistant and susceptible house flies. Pest. Biochem. Physiol. 12239.
Google Scholar
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Author notes
- J. G. Scott
Present address: Pesticide Chemistry and Toxicology Laboratory, Department of Entomological Sciences, University of California, 94720, Berkeley, California
Authors and Affiliations
- Division of Toxicology and Physiology, Department of Entomology, University of California, 92521, Riverside, California
J. G. Scott & G. P. Georghiou
Authors
- J. G. Scott
- G. P. Georghiou
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Scott, J.G., Georghiou, G.P. The biochemical genetics of permethrin resistance in the Learn-PyR strain of house fly.Biochem Genet 24, 25–37 (1986). https://doi.org/10.1007/BF00502976
- Received: 28 June 1985
- Revised: 25 September 1985
- Issue date: February 1986
- DOI: https://doi.org/10.1007/BF00502976