Hydrolysis of bile acid conjugates by Clostridium bifermentans (original) (raw)

Summary

Two strains of Clostridium bifermentans have been investigated for their ability to hydrolyse bile acid conjugates under conditions suited to further transformation of the free acids liberated. In batch fermentation at 0.5 g/l substrate concentration, growing cells effected the near-quantitative hydrolysis of glycodeoxycholate, taurodeoxycholate and taurocholate within 48 h; glycocholate was 88% hydrolysed. At substrate concentration greater than 1.0 g/l however, taurine conjugates were less well hydrolysed. Further transformation of the liberated cholic acid to deoxycholic acid and/or 7-ketodeoxycholic acid was achieved, but quantitative conversion was not observed.

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References

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Authors and Affiliations

1. Biotechnology Department, Massey University, Palmerston North, New Zealand
   R. H. Archer, R. Chong & I. S. Maddox

Authors

1. R. H. Archer
2. R. Chong
3. I. S. Maddox

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Archer, R.H., Chong, R. & Maddox, I.S. Hydrolysis of bile acid conjugates by Clostridium bifermentans.European J. Appl. Microbiol. Biotechnol. 14, 41–45 (1982). https://doi.org/10.1007/BF00508002

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• Received: 04 August 1981
• Issue date: March 1982
• DOI: https://doi.org/10.1007/BF00508002

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