Cell surface antigens on osteoclasts and related cells in the quail studied with monoclonal antibodies (original) (raw)
Summary
The properties of five monoclonal antibodies raised against isolated osteoclasts are described.
Osteoclasts were isolated from medullary bone of egglaying female quails. Mice were immunized with cell preparations consisting for about 10% of multinucleated osteoclasts. A large number of monoclonal antibodies against cell surface antigens were obtained, five of which were extensively characterized by their interactions with different tissues of the quail and their cross-reactivity with other species. Two monoclonals (OC 5.3 and OC 6.8), recognize surface antigens present on osteoclasts, monocytes, granulocytes and endothelial cells, but not on osteoblasts, osteocytes, fibroblasts, lymphocytes, erythrocytes and others. The three other monoclonal antibodies are specific for multinucleated osteoclasts in bone tissue but recognize some cell surface structures in other tissues. Antibody OC 6.9, which in bone tissue stains primarily the surface area of the osteoclast that is adjacent to the resorbing bone surface, also interacts with bile capillaries in the liver and with specific, but not yet identified parts of the nephron. The antibodies OC 6.1 and OC 6.3 interact with Kupffer cells in the liver and tissue macrophages of small intestine. In view of the possible fallacies inherent to the use of cell surface markers for the demonstration of cell relationship and origin, definite conclusions can not yet be made. The fact that the osteoclast, the Kupffer cell and the intestine macrophage are the only cells in bone, bone marrow, liver, kidney and intestine, that share the same surface antigen recognized by monoclonals OC 6.1 and OC 6.3, suggests, however, a common origin for osteoclasts and a number of well described tissue macrophages.
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References
- Astaldi GCB, Janssen MC, Landsdorp PM, Willems C, Zeylemaker WP, Oosterhof F (1980) Human endothelial culture supernatant (HECS): a growth factor for hybridomas. J Immunol 125:1411–1414
PubMed CAS Google Scholar - Barka T, Anderson PJ (1962) Histochemical method for acid phosphatase using hexazonium pararosalinin as coupler. J Histochem Cytochem 10:741–753
CAS Google Scholar - Beeken W, Mieremet-Ooms M, Ginsel LA, Leyh PC, Verspaget H (1984) Enrichment of macrophages in cell suspensions of human intestinal mucosa by elutriation centrifugation. J Immunol Methods 73:189–201
Article PubMed CAS Google Scholar - Burger EH, van der Meer JWM, van de Gevel JS, Gribnau JC, Thesingh CW, van Furth R (1982) In vitro formation of osteoclasts from long-term cultures of bone marrow mononuclear phagocytes. J Exp Med 156:1604–1614
Article PubMed CAS Google Scholar - Burger EH, Thesingh CW, van der Meer JWM, Nijweide PJ (1983) Development of osteoclasts in the mouse-localization of osteoclast precursors and role of bone forming cells. Proc 8th Int Conf Calcium Regulating Hormones, Japan. Excerpta Medica, Amsterdam New York Oxford, pp 125–130
Google Scholar - Bursuker I, Goldman R (1982) Distinct bone marrow precursors for mononuclear phagocytes expressing high and low 5′-nucleotidase activity. J Cell Physiol 112:237–242
Article PubMed CAS Google Scholar - Bursuker I, Goldman R (1983) On the origin of macrophage heterogeneity: a hypothesis. Reticuloendothel Soc 33:207–220
CAS Google Scholar - Chambers TJ (1980) The cellular basis of bone resorption. Clin Orthop 151:283–293
PubMed Google Scholar - De Bakker JM, De Wit AW, Koerten HK, Ginsel LA, Daems WTh (1985) On the origin of peritoneal resident macrophages. III. EM-Immunocytochemical studies on the origin of mouse peritoneal resident macrophages. J Submicrosc Cytol 17:153–159
PubMed Google Scholar - Fazekas de St Groth S, Scheidegger D (1980) Production of monoclonal antibodies: strategy and tactics. J Immunol Methods 35:1–21
Article Google Scholar - Göthlin G, Ericsson JLE (1973) On the histogensesis of the cells in fracture callus. Virchows Arch [Cell Pathol] 12:318–329
Google Scholar - Holtrop ME, King CJ (1977) The ultrastructure of the osteoclast and its functional implications. Clin Orthop 123:177–196
PubMed Google Scholar - Horton MA, Rimmer EF, Lewis D, Pringle JAS, Fuller K, Chambers TJ (1984) Cell surface characterization of the human osteoclast: phenotypic relationship to other bone marrow-derived cell types. J Pathol 144:281–294
Article PubMed CAS Google Scholar - Horton MA, Rimmer EF, Moore A, Chambers TJ (1985) On the origin of the osteoclast: the cell surface phenotype of rodent osteoclasts. Calcif Tissue Int 37:46–50
Article PubMed CAS Google Scholar - Köhler G, Milstein G (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256:495–497
Article PubMed Google Scholar - Marks SC (1983) The origin of osteoclasts. J Pathol 12:226–256
Google Scholar - Miller SC (1977) Osteoclast cell-surface changes during the egglaying cycle in japanese quail. J Cell Biol 75:104–118
Article PubMed CAS Google Scholar - Nijweide PJ, Van der Plas A (1983) Isolation, culture and characterization procedures for osteoblasts, periosteal fibroblasts and ostcoclasts. Calcif Tissue Int 35:A19
Google Scholar - Nijweide PJ, Blok J, VrijheidLammers T, Scherft JP (1984) The use of monoclonal antibodies against membrane antigens for the characterization of osteoclasts. Calcif Tissue Int 36 (Suppl 2):S7
Google Scholar - Ogawa T, Koerten HK, Daems WTh (1978) Peroxidatic activity in monocytes and tissue macrophages of mice. Cell Tissue Res 188:361–373
Article PubMed CAS Google Scholar - Oursler MJ, Bell LV, Clevinger B, Osdoby PH (1985) Identification of osteoclast-specific monoclonal antibodies. J Cell Biol 100:1592–1600
Article PubMed CAS Google Scholar - Page DT, Garvey JS (1979) Isolation and characterization of hepatocytes and Kuppfer cells. J Immunol Methods 27:159–173
Article PubMed CAS Google Scholar - Rifkin BR, Brand JS, Cushing JE, Coleman SJ, Sanavi F (1980) Fine structure of fetal rat calvarium; provisional identification of preosteoclasts. Calcif Tissue Int 31:21–28
Article PubMed CAS Google Scholar - Roth J, Bendayan M, Carlemalm E, Villiger W, Garavito M (1981) Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue. J Histochem Cytochem 29:663–671
PubMed CAS Google Scholar - Scheven BAA, Burger EH, Kawilarang-de Haas EWM, Wassernaar AM, Nijweide PJ (1985) Effects of ionizing irradiation on formation and resorbing activity of osteoclasts in vitro. Lab Invest (in press)
- Scott BL (1967) The occurrence of specific cytoplasmic granules in the osteoclast. J Ultrastruct Res 19:417–431
Article PubMed CAS Google Scholar - Slot JW, Geuze HJ (1981) Sizing of protein A-colloidal gold probes for immuno-electron microscopy. J Cell Biol 90:533–536
Article PubMed CAS Google Scholar - Teitelbaum SL, Kahn AJ (1980) Mononuclear phagocytes, osteoclasts and bone resorption. Miner Electrolyte Metab 3:2–9
Google Scholar - Teitelbaum SL, Stewart CC, Kahn AJ (1979) Rodent peritoneal macrophages as bone resorbing cells. Calcif Tissue Int 27:255–261
Article PubMed CAS Google Scholar - Thesingh CW, Burger EH (1983) The role of mesenchyme in embryonic long bones as carly deposition site for osteoclast progenitor cells. Dev Biol 95:429–438
Article PubMed CAS Google Scholar - Zambonin Zallone A, Teti A, Primavera MV (1982) Isolated osteoclasts in primary culture: First observations on structure and survival in culture media. Anat Embryol 165:405–413
Article PubMed CAS Google Scholar
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- Laboratory for Cell Biology and Histology, University of Leiden, Rijnsburgerweg 10, NL-2333 AA, Leiden, The Netherlands
P. J. Nijweide, T. Vrijheid-Lammers, R. J. P. Mulder & J. Blok
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- P. J. Nijweide
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Nijweide, P.J., Vrijheid-Lammers, T., Mulder, R.J.P. et al. Cell surface antigens on osteoclasts and related cells in the quail studied with monoclonal antibodies.Histochemistry 83, 315–324 (1985). https://doi.org/10.1007/BF00684377
- Received: 15 June 1985
- Issue Date: July 1985
- DOI: https://doi.org/10.1007/BF00684377